Highly motivated and results driven Ph.D. scientist in Biochemistry with 4 years of drug discovery experience in immune oncology, liver fibrosis and metabolic diseases. Areas of expertise are primarily in biochemical and cell based assay development to support SAR screening on kinases andreceptor targets.
Senior Scientist January 2016 to CurrentArcus Biosciences － Hayward, CA
Designed mammalian expression vectors to generate highly expression stable cell lines for immuno-oncology targets.
Developed cell-based functional assays to support SAR and selectivity screening on immuno-oncology targets.
Developed in-vitro serum and mouse functional assays to support pre-clinical trials.
Senior Scientist August 2013 to July 2015RUIYI Inc － Shanghai, China
Successfully developed high expression GPCR stable cell lines from CHO and 293 cell lines for binding and GPCR functional studies.
Led drug discovery program by developing ELISA, flow cytometry and functional cell based assays for screening novel antibodies on targets with liver fibrosis, metabolic diseases, oncology and pain indication.
Successfully obtained first-in class antibodies leads from hybridoma and phage display library for target with liver fibrosis and metabolic diseases indication.
Conducted cross-reactivity studies and characterized lead antibodies on mouse and human cell lines for patent filling and transition into pre-clinical trials.
Characterized the mechanism of action on lead antibodies by performing inverse agonism, neutral antagonism and schild plot analysis.
Prepared standard operation procedures and technical reports for IND filling, partnering and due diligence purposes.
Provided supervision and scientific guidance to associate scientists to meet project goals.
Senior Research Scientist January 2012 to July 2013Chempartner Co. Ltd － Shanghai, China
Led two scientists to work on multiple scientific projects including developing biochemical assays to measure and obtain nanomolar potency compounds and studies structure-activity relationship (SAR) of lead compounds on kinases enzyme targets.
Collaborated with PK/PD group to test kinases activation on mouse blood samples at various time points after dosing kinase activators for 72 hours.
Developed biochemical assays for high throughput screening compounds and mechanism of action studies on metabolic enzymes by measuring potency, reversibility and off-rate of lead compounds to facilitate metabolic diseases drug discovery.
Successfully obtain a lead compound targeting metabolic enzyme for client projects that lead to IND filling.
Developed FRET functional assays to setup a high-throughput screening platform for characterizing protein activity.
Prepared research proposals, scientific and financial cost reports for client's projects.
Graduate Research Assistant January 2006 to January 2011University of Michigan － Ann Arbor, MI
Drafted two NIH R01 grant applications, both ranked 92 percentile with 4 years funding of total one million USD.
Optimized the expression, purification and characterization of the recombinant expressed human mitochondrial metallonuclease protein complex and that involve in mitochondrial myopathy diseases using enzymatic techniques.
Investigated the molecular recognition of the RNA holoenzyme (RNase P) by performing bioinformatics analysis and enzyme kinetics studies.
Education and Training
Ph.D. : Chemical Biology, 2011University of Michigan － Ann Arbor, MI
Bachelor of Science : Biochemistry, 2006Eastern Michigan University － Ypsilanti, MIGPA: Graduated Magna Cum Laude GPA: 3.8/4.0
Howard MH, Lim WH, Fierke CA, and Koutmos M (2012) Mitochondrial Ribonuclease P Structure Provides Insight into the Evolution of Catalytic Strategies for Precursor-tRNA 5'-Processing. PNAS
Howard MH, Liu X, Lim WH, Klemm B, Fierke CA, Koutmos M and Engelke DR (2013) RNase P Enzyme: Divergent Scaffolds for A Conserved Biological Reaction. RNA Biology 10(6)
Lim WH, Chen Y, and Fierke CA. (2013) Substrate Recognition of the 5' leader of pre-tRNA by Bacterial Ribonuclease P (summited to RNA Journal for review)
Expertise in assays development including
filter-binding, fluorescence polarization, ELISA, Flow Cytometry, trFRET,
AlphaLISA, western blot analyses, PCR, cAMP/ERK signaling, beta-arrestin
reporter assays, receptor internalization, proliferation assays.
Extensive experience in cell culture including
co-transfection and selection of CHO cells, single cell cloning, clones
analysis by FACS to obtain high expression of CHO stable cell lines, and
adaptation of adherent 293 and CHO cells to suspension cells for antibodies
in maintaining and testing diverse cell lines such as hepatic stellate cell
lines, LX2, PANC-1, T47D, HL-60, K562 and HCT-116 cell lines.
In-depth knowledge with colorimetric, fluorescence and
luminescent plate readers and liquid handling system including Caliper,
Envision, SynergyNeo, ECHO550, Multidrop Combi, BioNEX, Bravo, HPLC, AKTA system, circular
dichroism (CD), fluorescence microscopy, and liquid scintillation.
Expertise in protein chemistry
including optimizing the expression, purification and characterization of
Outstanding Performance in Assay Development, Ruiyi Inc., 2014
Rackham Travel Grant Award, 2011
Bert W. Peet Award for outstanding accomplishment in Chemistry, 2006
Dean's List, for being ranked top 10 percent in overall academic accomplishment, 2002-2005
National Scholars Program, Regents Scholarship, and International Student Award for outstanding academic achievement, 2002-2006
Outstanding Service Award for excellent science course tutor, 2004