A highly experienced academic researcher looking for a new challenge in Science. Strong managementskills, ability to prioritize work to achieve objectives, deadlines and the ability to work effectively as part of teams. Discovered a novel function of EBV SM protein as an alternative-splicing factor, in addition to its essential role in viral lytic replication. Using RNA and DNA sequencing technology, we identified the role of several genes during viral replication. Developed a cell based high throughput assay for screening an antiviral compounds against EBV regulatory protein and find a drug that have an antiviral activity against EBV and closely related viruses.
Research experience and skills: 9 years of post-PhD experience in molecular virology especially in herpes virus gene regulation, experience with standard virology techniques including virus propagation, gene manipulation, infection and quantification. Good academic record with several papers and over 300 total citations. Excellent team player, 6 years of supervisory and mentoring experience. Molecular biology: gene and recombinant DNA cloning, qPCR, protein expression, DNA and RNA sequencing analysis, site directed mutagenesis, southern blot, northern blot, DNA and RNA isolation, Nuclear run on assay, RACE, RNA protein binding assay, and RNase protection assay. Biochemistry: recombinant protein expression and purification, separation of proteins by SDS-PAGE, immunoprecipitation and western blot analysis, Cell biology: cell culture, DNA and RNA transfection, flow cytometry, live cell imaging, reporter assay, immunofluorescence and RNAi (with siRNA, shRNA and lentivirus). Computer software: Microsoft Word, Excel, Power Point, Adobe Photoshop, Adobe Illustrator, Prism, and DNA alignment, assembly and analysis software such as Genious and vector NTI.
Research Assistant Professor November 2011 to November 2015University of Utah － Salt Lake City, USA, Utah
Dinesh Verma, Jacob Thompson, and Sankar Swaminathan.
Identification of an antiviral activity of an FDA approved drug by cell based screening assay that inhibits EBV production.
Manuscript in preparation for PNAS.
Jacob Thompson, Dinesh Verma, Da-Jiang Li, Tim Mosbruger, and Sankar Swaminathan.
Identification and Characterization of the Physiological Gene Targets of the Essential Lytic Replicative EBV SM Protein" Manuscript submitted in J.
Dinesh Verma, Da-Jiang Li, Brian Krueger, Rolf Renne, and Sankar Swaminathan.
Identification of the physiological gene targets of the essential lytic replicative KSHV ORF57 protein.
Da-Jiang Li, Dinesh Verma, Tim Mosbruger and Sankar Swaminathan "CTCF And Rad21 Act as Host Cell Restriction Factors for Kaposi's Sarcoma-Associated Herpesvirus (KSHV) Lytic Replication By Modulating Viral Gene Transcription." PLOS Pathogens.
Dinesh Verma, Euan A Kim and Sankar Swaminathan.
A cell-based screening assay for antiviral compounds targeting the ability of herpesvirus post-transcriptional regulatory proteins to stabilize viral mRNAs.
Da-Jiang Li, Dinesh Verma and Sankar Swaminathan.
Binding of cellular export factor REF/Aly by KSHV ORF57 protein is not required for efficient KSHV lytic replication.
2012 September; 86:9866-74.
Dinesh Verma, Swarna Bais, Melusine Gaillard and Sankar Swaminathan.
Epstein-Barr virus SM protein utilizes cellular splicing factor SRp20 to mediate alternative splicing.
2010 November; 84:11781-11789.
Zhao Han, Dinesh Verma, Chelsey Hilscher, Dirk P.
Dittmer, and Sankar Swaminathan.
General and target-specific RNA binding properties of Epstein Barr virus SM post-transcriptional regulatory protein.
2009 November; 83: 11635-11644.
Dinesh Verma, Chen Ling, Eric Johannsen, Tirumuru Nagaraja, and Sankar Swaminathan.
Negative autoregulation of Epstein-Barr virus (EBV) replicative gene expression by EBV SM protein.
2009 Aug; 83(16):8041-8050.
Dinesh Verma, Sankar Swaminathan.
Epstein-Barr virus SM protein functions as an alternative splicing factor.