Proficient in PCR, qPCR, and Next Generation sequencing
Illumina Hiseq 2500 and 2000
Illumina Genome Analyzer II
Hamilton Robotic MICROLAB STAR liquid handlers
Beckman Coulter Biomek
Qiagen QIAgility and QIAcube
Agilent Bioanalyzer 2100
Covaris E220 and LE220 ultrasonicators
PerkinElmer Sciclone G3 and Zephyr liquid handlers
PerkinElmer Caliper LabChip GX
Applied Biosystems 3730/3730XL
Next Generation Sequencing Supervisor06/2014 － CurrentGeneDxGaithersburg, MD
Specializes in Next Generation DNA sequencing on the Illumina platform (Hiseq 2000, Hiseq 2500, Hiseq4000, Miseq) to detect disease-causing mutations in a CAP and CLIA certified clinical laboratory Uses manual and automated methods to prepare DNA libraries for cluster generation on the Illumina cBot Plans and organizes an average of 40 sequencing runs weekly Troubleshoots technical issues on the Hiseqs and failed sequencing runs Validates new equipment and methods Trains new lab technicians on Next Generation sequencing Writes and edits Standard Operation Procedures (SOPs) Keeps up with maintenance of Hiseqs and cBots.
Exome Technical Specialist06/2013 － 05/2014
Performed capture-based whole human exome libraries using the Agilent SureSelect XT2 kit.
Used the Illumina Hiseq 2000 and 2500 to sequence whole human exomes.
Cleared a backlog of over 800 samples within 3 months and decreased turn-around time.
Wrote and updated QA/QC documents including Standard Operating Procedures (SOPs), training and reagent logs, and validation reports.
Sequenced clinical samples on the Illumina Genome Analyzer II, Hiseq 2000 and Hiseq 2500 Prepared DNA libraries of clinical samples by multiplex PCR with manual and automated techniques Quantified DNA using qPCR on the LightCycler and Stratagene systems Amplified DNA using manual and automated methods of Polymerase Chain Reaction (PCR) Performed gel electrophoresis with agarose gels and the Caliper LabChipGX Conducted Sanger sequencing on the Applied BioSystems(ABI) 3730/3730XL Prepared restriction enzyme digests Trained new lab technicians on PCR, DNA library preparation and Next Generation Sequencing Assisted with writing and editing SOPs Kept up with daily and monthly maintenance of lab equipment.
Laboratory Technician I08/2010 － 03/2011SeraCare Life Sciences, IncGaithersburg, MD
Experienced using sterile technique in a cGMP/GLP environment Inventoried and aliquoted incoming samples to perform DNA extraction, PCR and SDS gel-electrophoresis Pulled and aliqouted requisitioned samples from mechanical and LN2 freezers to prepare for shipping Performed general laboratory maintenance Kept accurate inventory of cryopreserved samples and performed quality control checks on them Investigated missing specimens using Biological Specimen Inventory (BSI) and hardcopy data Assisted with data entry to generate reports Monitored freezers, room temperatures, and responded to freezer alarms.
8/2017Masters of Science:Mount St. Mary's University - Biotechnology and ManagementEmmitsburg, MDBiotechnology and Management
5/2010Bachelor of Science:University of Maryland - BiochemistryCollege Park, MDBiochemistry
AWARDS: Award for Team Excellence, GeneDx 3/2014 Awarded to the Exome team for work ethic, dedication, and positive outlook in continuing to go above and beyond to ensure quality patient testing PROFESSIONAL ASSOCIATIONS: Alpha Chi Sigma Professional Chemistry Fraternity, Indianapolis, IN Officer at University of Maryland Chapter, 5/2009-5/2010 Planned and ran major events consisting of 40-50 people Organized fundraiser for the Avon Walk for Breast Cancer Collaborated with fellow officers to manage day to day activities of Alpha Chi Sigma, hold weekly meetings and delegate tasks to other members Tutored students in introductory chemistry, biology and biochemistry courses