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Co-Director of Lipidomics and Metabolomics Core Resume Example

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CO-DIRECTOR OF LIPIDOMICS AND METABOLOMICS CORE
Professional Overview

Expertise in separation science, especially for molecule structure elucidation and isolate target molecules, and method development using HPLC, GC, CE and MS/MS 

Core Qualifications
  • Analytical Chemistry
  • Drug Metabolism in vivo and in vitro
Education
2007
Peking UniversityBeijing, ChinaPh.D.: Analytical Chemistry
2001
Peking UniversityBeijing, ChinaBachelor of Science: Chemistry
Experience
08/2010 - Current
Brigham and Women's Hospital, Harvard Medical School － Boston, MACo-Director of Lipidomics and Metabolomics Core
  • Structural identification and quantification of bioactive lipids and their metabolites in biological or clinical samples by ABSciex Qtrap5500 LC-MS/MS, LC-UV, and GC-MS.
  • In vivo and in vitro metabolism of bioactive agents, especially pro-resolving lipid mediators, elucidating the structures by LC-MS/MS
  • Managed actively in different collaboration projects, planning, organizing
09/2007 - 08/2010
NIA, National Institute of Health － Bethesda, MAVisiting Fellow
  • Essential Fatty acid metabolism studies.
  • Developed a kinetic model to estimated liver synthesis-secretion rates of docosahexaenoic acid (DHA) from *-linolenic acid in rats by the stable isotope labeling GC-MS techniques.
09/2002 - 07/2007
Ph D. Candidate, College of Chemistry, Peking University － Beijing
  • Developed new LC-MS methods and protocols for the identification and quantification of molecular lipids in complex matrices
  • Applied Capillary Electrophoreis (CE) to analysis of phospholipids
  • Screened a diagnostic biomarker for clinical disorders by HPLC-MS
  • Analyzed herbal medicines by CE, GC, GC-MS, HPLC, and HPLC-MS
  • Chiral Separations by CE and HPLC Qualification and Skills
Skills
  • Experienced in lipidomics and metabolomics analysis
  • Proficient in structure elucidation and quantification for organic molecules and biomolecules with mass spectrometry
  • Experienced with a variety of analytical instruments, such as GC, GC-MS, HPLC, HPLC-MS, CE, CE-MS, UV, and IR.
  • Extensive knowledge of current development of chromatography and mass spectrometry
  • Familiar with numerous software packages
Publications

1.Fei Gao, Xinkui Tian, Dawei Wen, Jie Liao, Tao Wang and Huwei Liu, Analysis of phospholipid species in rat peritoneal surface layer by liquid chromatography/electrospray ionization ion-trap mass spectrometry, Biochim. Biophys. Acta 2006, 1761, 667–676  

 

2.Fei Gao, Juan Dong, Wei Li, Tao Wang, Jie Liao, Yiping Liao and Huwei Liu, Separation of phospholipids by capillary zone electrophoresis with indirect ultraviolet detection, J. Chromatogr. A 2006, 1130, 259-264. 

 

3.Fei Gao, Zhengxiang Zhang, Xiaofang Fu, Wei Li, Tao Wang, Huwei Liu, Analysis of phospholipids by nonaqueous capillary electrophoresis with on-line electrospray ionization mass spectrometry, Electrophoresis, 2007, 28, 1418-1428. 

 

4.Fei Gao, Dale Kiesewetter, Lisa Chang, Kaizong Ma, Jane M. Bell, Stanley I. Rapoport, Miki Igarashi, Whole body synthesis-secretion rates of long-chain n-3 polyunsaturated fatty acids from circulating unesterified -linolenic acid in unanesthetized rats, J. Lipid Res 2009, 50, 749-758.  

 

5.Fei Gao, Dale Kiesewetter, Lisa Chang, Kaizong Ma, Stanley I. Rapoport, Miki Igarashi, Whole-body synthesis-secretion of docosahexaenoic acid from circulating Eicosapentaenoic acid in anesthetized rats, J. Lipid Res 2009, 50, 2463-2470 

 

6.Fei Gao, Dale Kiesewetter, Lisa. Chang, Stanley. I. Rapoport, and Miki. Igarashi, Quantifying conversion of linoleic to arachidonic and other n-6 polyunsaturated fatty acids in unanesthetized rats. J Lipid Res 2010, 51, 2940-2946 

 

7.Xingwei Zhe, Fei Gao, Honggang Nie, Xinkui Tian, Wei Chen, Huwei Liu, Bent Lindolm, Janas Axelsson, Tao Wang, Increased dialysate levels of phospholipids containing unsaturated fatty acid are associated with increased peritoneal transport rate, Am J Nephrol. 2008, 28, 1007-1013 

 

8.Miki Igarashi, Fei Gao, Hyung-Wook Kim, Kaizong Ma, Jane M. Bell, Stanley I. Rapoport, Dietary n-6 PUFA deprivation for 15 weeks reduces arachidonic acid concentrations while increasing n-3 PUFA concentrations in organs of post-weaning male rats, Biochim. Biophys. Acta 2009, 1791, 132-139 

 

9.Ramon, S., Gao, F., Serhan, C. N., and Phipps, R. P. (2012) Specialized proresolving mediators enhance human B cell differentiation to antibody-secreting cells. J Immunol 2012, 189, 1036-1042 

 

10.Stanley I. Rapoport, Miki Igarashi, Fei Gao, Quantitative contributions of diet and liver synthesis to docosahexaenoic acid homeostasis Prostaglandins Leukot Essent Fatty Acids 2010, 82, 273-276 

 

11.Miki Igarashi, Kaizong Ma, Fei Gao, Hyung-wook Kim, Dede Greenstein, Stanley I. Rapoport, Jaqadeesh Rao, Brain lipid concentration in bipolar disorder, J Psychiatr Res. 2010, 44, 177-182. 

 

12.Xiaofang Fu, Lin Huang, Fei Gao, Wei Li, et al. Carboxymethyl chitosan-coated capillary and its application in CE of proteins, Electrophoresis 2007, 28, 1958-1963. 

 

13.Shehong Li, Chiyang He, Fei Gao, Daobing Li, Zheng Chen, Huwei Liu, Kean Li and Feng Liu Extraction and determination of morphine in compound liquorice using an aqueous two-phase system of poly(ethylene glycol)/K2HPO4 coupled with HPLC Talanta 2007, 71, 784-789. 

 

14.Zhe Meng, Dawei Wen, Dandan Sun, Fei Gao, Wei Li, Yiping Liao, Huwei Liu, Rapid determination of C12-C26 non-derivatized fatty acids in human serum by fast gas chromatography, J. Sep. Sci. 2007, 30, 1537-1543.  

 

15.Xiaofang Fu, Yong Zhang, Shenhua Shi, Fei Gao, Dawei Wen, Wei Li, Yiping Liao, Huwei Liu, Fragmentation study of hexanitrostilbene by ion trap multiple mass spectrometry and analysis by liquid chromatography/mass spectrometry, Rapid Commun. Mass Spectrom. 2006, 20, 2906-2914 

 

16.Suxuan Gong, Fujia Liu, Wei Li, Fei Gao, Chunjin Gao, Yiping Liao and Huwei Liu, Separation of hydrophobic solutes by organic-solvent-based micellar electrokinetic chromatography using cation surfactants , J. Chromatogr. A 2006, 1121, 274-279 

 

Presentations

1.Fei Gao, Miki Igarashi, Stanley I. Rapoport, Upregulated liver synthesis and secretion of docosahexaenoic and arachidonic acids in rats on DHA-free diet, 101st AOCS Annual Meeting & Expo, 2010, Phoenix, AZ, USA.  

 

2.Fei Gao, Yiping Liao, Tao Wang, Huwei Liu, Analysis of Phospholipids by Nonaqueous Capillary Electrophoresis with On-line Electrospray Ionization Mass Spectrometry, 21th International Symposium on Microscale Bioseparations, 2007, 1, Vancouver, Canada 

 

3.Fei Gao, Juan Dong, Wei Li, Tao Wang, Huwei Liu, Determination of phospholipids in human serum by capillary zone electrophoresis with indirect ultraviolet detection 20th International Symposium on Microscale Bioseparations, 2006, 1, Amsterdam, Netherlands.  

 

4.Fei Gao, Tao Wang, Yiping Liao, Huwei Liu, Analysis of Phospholipid Species in Peritoneal Surface Layer by Liquid Chromatography - Electrospray Ionization Tandem Mass Spectrometry, 11th International Beijing Conference and Exhibition on Instrumental Analysis, 2005, 10, Beijing, China 

 

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Resume Overview

Companies Worked For:

  • Brigham and Women's Hospital, Harvard Medical School
  • NIA, National Institute of Health
  • Ph D. Candidate, College of Chemistry, Peking University

School Attended

  • Peking University

Job Titles Held:

  • Co-Director of Lipidomics and Metabolomics Core
  • Visiting Fellow

Degrees

  • Ph.D. : Analytical Chemistry
    Bachelor of Science : Chemistry