Biological Laboratory TechnicianUnited States Department of Agriculture/Agricultural Research Service ARS Dale Bumpers National rice Research Center DBNRRC － Stuttgart, AR
Supervisor: Yulin Jia (870) 672-9300.
02/2014 to 10/2015
U.S. Army/ U.S. Army MEDCOMM
Medical Research Institute for Chemical Defense USAMRICD － Edgewood, MD
Supervisor: John McDonough, Ph.D.
410) 436-1942 Pay grade: GS-09-01 Contributed to MRICD mission and to Toxicology/pharmacology research in the following broad areas: Development of medical countermeasures against chemical warfare agents (Nerve Agents) Testing of pharmaceutical pre-treatments (oximes) to protect against nerve agent intoxication Development of nerve-agent pharmaceutical treatment regimens Screening of new pharmaceutical compounds for anti-seizure activity in nerve agent intoxication Oversees and conducts setup of animal experiments Prepare pharmaceutical compounds at the proper concentration for injection Oversees and conducts rodent surgeries, injections, and blood draws.
Utilizes trans-cortical electroencephalographic data recording devices Summarize and maintain data in computerized database and laboratory notebooks Non-Human Primate handling and pharmacological testing Assists in the proper disposal of Hazardous wastes generated by the laboratory Data collection and statistical analysis of data Microscopic slide image processing using image J® software Controlled drug custodian Security clearance: SECRET.
11/2011 to 02/2014
Biological Science Laboratory TechnicianMedical Research Institute for Chemical Defense USAMRICD － Edgewood, MD
United States Department of Defense/U.S.
Army medical Command (MEDCOMM) Supervisor: John McDonough, Ph.D.
410) 436-1942 Contact permission: Yes Pay grade: GS-07-04 Rodent (Rat & Guinea Pig) handling and husbandry Rodent injections, blood draws, and surgery Non-Human Primate pharmaceutical injections, blood draws, and post exposure monitoring Controlled drug custodian Drug preparation and dilutions Data collection and statistical analysis of data Scientific writing Laboratory equipment custodian Laboratory supply requisitions Security clearance: SECRET.
11/2009 to 11/2011
Biological Science Research Technician
United States Department of Agriculture (USDA)/Agricultural Research Service (ARS) Beneficial Insect Introduction Research Laboratory - Emerald Ash Borer (EAB) Project 501 South Chapel Street * Newark DE, 19713 Supervisor: Jian Duan, Ph.D.
302) 731-7330 x249 Pay Grade: GS-05-00 to GS-06-02 Certified to work in APHIS Quarantine Containment Facilities DNA extractions, PCR reactions, Gel electrophoresis, Gel extractions, PCR clean-up, sequencing reactions, dye removal reactions Behavioral bio-assays Development of a standardized rearing protocol for EAB larvae/adults Development of standardized rearing protocols for EAB bio-control agents: Documentation and archiving of experimental data & other required administrative records Collection and statistical analysis of experimental data to include: Scientific Writing Design & fabrication of experiment support equipment 4-arm Olfactometer Automatic humidification system for rearing chambers Rearing chambers and system for rearing of Emerald Ash Borer in the laboratory Greenhouse operations & greenhouse systems installation and maintenance Greenhouse production of tropical ash (Fraxinus uhdei) trees for Emerald ash borer (Agrilus planipennis) rearing purposes Purchasing agent for EAB project: Responsible for purchasing all required laboratory and field supplies for the EAB project Collateral Duty Safety Officer - Beneficial Insect Introduction Research Laboratory Graduate OSHA 6000 course Security clearance: CONFIDENTIAL.
06/2009 to 10/2009
Molex® Inc － Maumelle, AR
Supervisor: George Livingston (501) 803-1169 Chemical analysis and process control of electrolytic plating bath solutions Tin, Lead, Nickel, Copper, and Gold Inductively coupled plasma atomic emission spectroscopy Chemical analysis and process control of electro-less plating bath solutions Cooper, Nickel, and Gold EDTA, iodometric, and acid/base titration Precious metal (gold) assays.
06/2008 to 01/2009
Chemical Laboratory Technician
08/2005 to 01/2008
Graduate Teaching Assistant/Laboratory AssistantUniversity of Central Arkansas － Conway, AR
David Dussourd (501) 450-5921 Laboratory instructor for BIOL 1440, freshman biology for biology majors Tutored students in basic biological principles covered in lecture Graded writing assignments Responsible for laboratory preparation for ~ 20 lab sections of BIOL 1440 Oversee laboratory material setup Developed new laboratory exercises for BIOL 1440 (Freshman Biology) Responsible for laboratory equipment preventive maintenance and repair.
01/2001 to 01/2008
Contributed to DBNRRC mission and research in the following broad areas: Conducted experimental assays to determine pathogenicity of Magnaporthe oryzae (Rice Blast) fungus to a wide variety of rice cultivars Supported plant pathology staff scientists and and visiting scientists with daily research needs Greenhouse manager for the Plant-Pathology Research group Supported laboratory staff by maintaining glassware and lab consumables; maintaining laboratory notebooks and computer records; troubleshooting equipment and resolving general laboratory problems; ensuring good laboratory practices; complying with safety and health regulations, and assisting with special projects Obtained accurate pathogenicity data in 3 unique assays utilizing over 1300 plants Data from assays used by Primary Investigator to identify rice genes providing Blast resistance Identified genes used by rice breeders to created blast resistant rice varieties for agricultural use Planted, nurtured, and harvested rice cultivars in order to maintain seed inventory for experimental purposes Inoculated, cultured, and preserved viable populations of various Magnaporthe oryzae race-subtypes for experimental testing on selected rice cultivars Ensured greenhouse facility was maintained in a safe, pest and pathogen free condition Assisted in the proper disposal of Hazardous wastes generated by the laboratory and greenhouse Summarized and maintained data in computerized database and laboratory notebooks Assisted each visiting student and scientist to ensure program success as needed Edited manuscripts and abstracts as needed.
07/1998 to 09/2000
RankPennsylvania Army National Guard － Berwick, PA
Gunner M1-A1 Abrams Main Battle Tank.
Honorable Discharge, June 26 1996.
VA service connected disability.
10/1993 to 06/1996
Technical InstructorU.S. Navy
10/1987 to 10/1993
Fire Control TechnicianU.S. Navy Newport-News, VA － San Diego, VA CA
Highest rank attained: E-6 Held TOP SECRET (SBI) security clearance Supervised division of nine Fire Control Technicians on-board attack submarine USS Asheville SSN-758 Scheduled and supervised preventive maintenance Responsible for the tactical proficiency of the fire control division Responsible for the combat readiness of the fire control system Systematic troubleshooting and repair of the AN-BSY-1 submarine fire control system Prepared periodic performance evaluations of divisional personnel.
Lead InstructorNaval Submarine School － Groton, CT
Weapon Handling and Launch Equipment course (SEAWOLF Project) Work Center Supervisor: AN-BSY-1(v) maintenance trainer (3rd Flight vertical launch LOS ANGLES ) Responsible for development and revision of AN/BSY-1 Pipeline course curriculum Technical instructor: AN-BSY-1 Fire control system(s) operation and maintenance Instructed new recruits in operation and corrective maintenance of the AN-BSY/1 fire-control system.
Master of Science: Biology Chemical Ecology, Plant-Insect interactions, & Plant EcophysiologyUniversity of Central Arkansas － Conway, ARBiology Chemical Ecology, Plant-Insect interactions, & Plant Ecophysiology
Bachelor of Science: Biology ChemistryMansfield University of Pennsylvania － Mansfield, PABiology Chemistry
Duan, J. J., T. Watt, and C.B. Oppel. (2011). "Critical Rearing Parameters of Tetrastichus planipennisi (Hymenoptera: Eulophidae) as Affected by Host-Plant Substrate and Host-Parasitoid Group Structure". J. Econ. Entom. 105(3): 792-801. Duan, J. J., T. Watt, and C.B. Oppel. (2011). "An alternative host plant-based method for laboratory rearing of emerald ash borer to produce larval parasitoids for biological control". In: USDA APHIS Emerald Ash Borer Technology and Development Meeting, Wooster, OH, October 12 - 14, 2011. Duan, J. J., Oppel, C. B., Ulyshen, M. D., Bauer, L. S. and LeLito J. (2011). "Biology and life history of Tetrastichus planipennisi (Hymenoptera: Eulophidae), a larval endoparasitoid of the emerald ash borer (Coleoptera: Buprestidae)". Florida Entomol. 94:4 (933-940) Oppel, C.B., D. Dussourd, and U. Garimella (2009). "Microwave visualization of a plant defense and insect counter ploy." Journal of Chemical Ecology 35: 625-634 Oppel, C. B. (2008). "Microwave visualization of a plant defense and insect counter-ploy: Alkaloid distribution in Lobelia leaves trenched by a Plusiine caterpillar". Master of Science Thesis, University of Central Arkansas, Conway, AR 722034 POSTER PRESENTATIONS Development and validation of a rat model of delayed treatment for nerve agent intoxication". Hilary S. McCarren, Sergiu Costinas, Emily Dunn, Wafae Driwech, Ashley Hubbard, Cecelia Jackson, Ryan Krempel, Emylee McFarland, Craig Oppel, John H. McDonough. Society for Neuroscience Annual Meeting, October 17-21 2015, Chicago, IL. Rat and Guinea Pig Models of Nerve Agent Intoxication to Evaluate Delayed Treatment with Novel Anticonvulsants." Hilary S. McCarren, Sergiu Costinas, Emily Dunn, Wafae Driwech, Ashley Hubbard, Cecelia Jackson, Ryan Krempel, Emylee McFarland, Craig Oppel, John H. McDonough.. CounterACT Network Research Symposium, June 15-17 2015, New York, NY. Developmental changes in brain and peripheral tissue acetylcholinesterase in male and female Sprague-Dawley rats." Miller, S.,. Dailey, M., Koenig, J., Driwech, W., Lakin, J., McFarland, E., McMonagle, J., Oppel, C., Winter, K., McDonough, J.H... 19th Biennial Medical Defense Bioscience Review:12-15 May, 2014, Hunt Valley, MD The effectiveness of pyridostigmine and scopolamine in enhancing survival and recovery from the lethal effects of five nerve agents." J. McDonough, T. Myers, J. Langston, M. Wegner, K. Kan, C. Oppel, S. Miller, B.M. Burgan, T. Dao, W. Driwech, D.W. Kahler, J. Lakin, J. Leuschner, J.R. Makar, E. McFarland, K. Winter. CBD S&T Virtual Conference: November 2014. POSTER PRESENTATIONS Rat and guinea pig models of nerve agent intoxication to evaluate delayed treatment with novel anticonvulsants." McDonough, J.H., Black, K., Driwech, W., McFarland, E., McMonagle, J., Miller, S. Oppel, C., and Winter, K. 8th Annual NIH Counteract Symposium; 17-19 June 2014, Denver, CO. Rat and guinea pig models of nerve agent intoxication to evaluate delayed treatment with novel anticonvulsants." McDonough, J.H., Black, K., Driwech, W., McFarland, E., McMonagle, J., Miller, S., Oppel, C., and Winter, K. 7th Annual NIH Counteract Symposium; 25-27 June 2013, Washington, DC.
AWARDS, SCHOLARSHIPS & GRANTS Pennsylvania Higher Education Administration: Science Scholarship - 2003-2005 Stanley H. Nauman memorial Scholarship -Two awards 2004 & 2005 Mansfield University outstanding biology senior - spring 2005 Arkansas Game & Fish Commission conservation scholarship - 2006 to 2008 Sigma Xi grant in aid of research $1,000.00- January 2006 Summer research fellowship $2,800.00, University of Central Arkansas- Summer 2006 Recognition of Excellence (Biology) - Educational Testing Services, June 2009: Awarded to individuals scoring in the top 15% nationally on the PRAXIS II Biology content knowledge examination Baltimore Excellence in Federal Career Awards (Gold) - Outstanding Para-Professional (Non-Supervisory), Technical, Scientific and Program Support. May 2015
MILITARY SERVICE AWARDS United States Navy Submarine Warfare insignia Navy Achievement Medal: One award Navy Good Conduct Medal: Two awards National Defense Medal: One award General Jeb Stuart Medal (Pennsylvania Army National Guard): One Award Navy Meritorious Unit Commendation ribbon United States Army Marksman Pistol Badge Army Non-Commissioned Officer Training ribbon Army Service ribbon