Animal model and animal care, Research & Development.
Sr Scientist, Charles River Lab, Microbiome & Molecular Diagnostics, December 2015-CurrentWilmington, MA
Initiated and developed a microbiome testing platform and service at CRL.
Utilized next-generation sequencing (NGS) to discover novel rodent viruses.
Extensively used AAV vector to clone many constructs and generated disease animal models.
Designed and cloned positive template controls for PCR assays.
Designed new expanding Charles River's TaqMan PCR assays and revised existing PCR assays for animal infectious
Provided scientific support for mammalian cell culture, transfection, viral vector production/purification, and viral
Maintained viral vector frozen stocks inventor.
Led R&D activities within the Molecular Diagnostics Department through effectively interacting with multi-
disciplinary project teams.
Supervised and mentored junior team members on animal disease testing and alternative health monitoring options
based on past knowledge and experiences.
Responsible for development, troubleshooting, qualification, and testing of qPCR-based assays, IFA, and Multiplexed
Fluorometric Immuno Assay.
Conducted novel gene therapy experimental designs and morphological/clinical pathology evaluation.
Implemented emerging technologies in viral vector development and gene therapy.
Developed and optimized pathological tissue collection protocols and assessment schemes in preclinical species.
Successful developed pharmacokinetic methods evaluation of drug metabolism and toxicodynamic properties from in
Investigated rat/mice gut microbiome changing after relocations.
Served as a scientist member in Institutional Animal Care and Use Committee (IACUC) for animal research protocols.
Prepared, revised, or reviewed test methods and technical datasheets.
Conducted research projects, compile data and prepare papers for platform presentations/posters at relevant conferences.
Support the commercial testing laboratory with assistance: protocol troubleshooting, optimization, results analysis and
Possess extensive problem-solving experience in providing technical work in many kinds of lab animal species, such
as gavage dosing, IP, IM, and SC injections and tissue collections after necropsy.
Great working knowledge of laboratory animal equipment and supplies, including cage wash, autoclave, and caging
systems for a variety of common laboratory animals and personal protective equipment requirements.
Closely work with internal and external customers to understand PCR sampling, test methods, results interpretation,
and next steps when a positive infectious agent is found.
Possess a knowledge base regarding infectious agents of concern for multiple lab animal species across CR's portfolio,
including rodents, non-human primates, zebrafish, etc.
Knowledgeable of health monitoring testing options including Exhaust Air Dust Testing (EAD).
Fundamental understanding of other laboratory testing services offered through RADS, GEMS and CR inbred/hybrid
Co-travel with the field sales team to perform client site visits to revise the health surveillance program in their vivarium,
customized presentations, and questions addressing.
Postdoctoral Fellow/ Veterinary Resident, Massachusetts Institute of Technology, March 2012-December 2015Cambridge, MA
A well-trained veterinarian with the specialty of lab animal medicine.
As part of my residency in laboratory animal
medicine, I am part of a team of veterinarians that provides medical care for animals at MIT.
Training areas and daily activities included: Disease surveillance, pathology of laboratory animals, clinical case workup,
husbandry and enrichment practices, facility design, protocol, and standard operating procedure development,
regulatory oversight, surgery.
As a postdoctoral fellow investigated the effect of interleukin-22 on DNA damage and dysplasia in a murine model of
colitis-associated cancer induced by Helicobacter hepaticus.
Examined effects of colonization of gnotobiotic Swiss Webster mice with Helicobacter bilis.
Research assistant, Purdue University, June 2005-March 2012WestLafayette, IN
Studied about T cells immunology, such as regulatory T cells (Tregs) and Th17 cells in mucosal immunology.
Used inflammatory bowel disease/colitis disease and experimental autoimmune encephalomyelitis animal models.
Investigated colon cancer, breast cancer, and melanoma enriched T cell biology.
Education and Training
CERTIFICATEDiplomate American College of Laboratory Animal Medicine, 2018
Ph.DPurdue University, , West LafayetteIN2011
Master of Veterinary PathologyChina Agricultural University, , BeijingChina2004
2020-current: Member, Association of Primate Veterinarians
2018-current: Member, American College of Laboratory Animal Medicine
2012-current: Member, American Association of Laboratory Animal Science
2012-2015: Student Member, Society of Toxicological Pathology
2009-2015: Student Member, American Association of Immunologists, As one of the contributors for the book- Avian Disease Diagnosis and Prevention (the First edition, China Agricultural
University Press, 2003)
REVIEWING ACTIVITIES FOR SCIENTIFIC JOURNALS
Cellular & Molecular Immunology
Journal of Immunology
Journal of Interferon & Cytokine Research
Journal of Clinical Immunology
Clinical & Experimental Immunology
(Total refereed publications: 23; Total Citations = 792 (as of 5/23/2020 by Google Scholar);h-index
H-index = h publications with at least h citations;
I10-Index = Number of publications with at least 10
Mark T Whary, Chuanwu Wang, Catherine F Ruff, Mallory J DiVincenzo, Caralyn Labriola, Lillian Ge, Yan Feng,
Zhongming Ge, Vasu Bakthavatchalu, Suresh Muthupalani, Bruce H Horwitz, James G Fox. Effects of Colonization of
Gnotobiotic Swiss Webster Mice with Helicobacter bilis. Comp Med. 2020 Apr 29. doi: 10.30802/AALAS-CM-19-
Zhongming Ge, Alexander Sheh, Yan Feng, Sureshkumar Muthupalani, Lili Ge, Chuanwu Wang, Susanna Kurnick,
Anthony Mannion, Mark T Whary, James G Fox. Helicobacter pylori-infected C57BL/6 mice with different
gastrointestinal microbiota have contrasting gastric pathology, microbial, and host immune responses. Scientific reports,
2018, 8 (1), 1-15. (Cited by 10)
C Wang, G Gong, A Sheh, S Muthupalani, EM Bryant, DA Puglisi, H Holcombe, EA Conaway, NAP Parry, V
Bakthavatchalu, SP Short, CS Williams, GN Wogan, SR Tannenbaum, JG Fox, BH Horwitz. Interleukin-22 drives nitric
oxide-dependent DNA damage and dysplasia in a murine model of colitis-associated cancer. Mucosal immunology,
2017, 10 (6), 1504-1517. (Cited by 26)
Chuanwu Wang, Michelle Wunderlich, Kenneth Henderson. Murine roseolovirus, historically known as murine thymic
lymphotropic virus. Journal of virology, 2017, 91 (18), e00943-17. (Cited by 1)
Naresh S Redhu, Vasudevan Bakthavatchalu, Evan A Conaway, Dror S Shouval, Amy Tsou, Jeremy A Goettel, Amlan
Biswas, Chuanwu Wang, Michael Field, Werner Muller, Andre Bleich, Ning Li, Georg K Gerber, Lynn Bry, James G
Fox, Scott B Snapper, Bruce H Horwitz. Macrophage dysfunction initiates colitis during weaning of infant mice lacking
the interleukin-10 receptor. Elife 2017 (6), e27652. (Cited by 9)
Mark T Whary, Chuanwu Wang, Lili Ge, Yan Feng, Zhongming Ge, Vasudevan Bakthavatchalu, Sureshkumar
Muthupalani, Bruce Horwitz, James G Fox. Activation of Gut-Associated Tertiary Lymphoid Tissue in Gnotobiotic
Swiss Webster Mice Distinguishes Helicobacter Bills, a 'Provocateur Pathosymbiont', from Segmented Filamentous
Bacteria. Gastroenterology, 2017, 152 (5), S1000.
Guanyu Gong, Chuanwu Wang, Sureshkumar Muthupalani, Nicola M Parry, Vasudevan Bakthavatchalu, Gerald
Wogan, Steven R Tannenbaum, James G Fox, Bruce Horwitz. IL-22-Dependent iNOS Induction Drives DNA Damage
in a Murine Model of Colitis-Associated Cancer. Gastroenterology, 2016, 150 (4), S125
Naresh Redhu, Dror Shouval, Vasudevan Bakthavatchalu, Chuanwu Wang, Evan Conaway, Jeremy Goettel, Matthias
Mack, Andre Bleich, Lynn Bry, James Fox, Scott Snapper, Bruce Horwitz. Microbiota drives inflammation by altering
intestinal lamina propria macrophage phenotype in a novel IL10R-deficient model of very early onset IBD.
Inflammatory Bowel Diseases 2016, 22 (suppl_1), S51-S51. (Cited by 6)
Z Ge, Y Feng, A Sheh, S Muthupalani, C Wang, L Ge, JG Fox. Influence of Gastrointestinal Microbiota on Pathogenic
Potential of Helicobacter pylori in C57BL/6 Mice. Gastroenterology 2015, 148 (4), S-688.
Z Ge, Y Feng, A Sheh, S Muthupalani, C Wang, L Ge, JG Fox. Influence of Gastrointestinal Microbiota on Pathogenic
Potential of Helicobacter pylori in C57BL/6 Mice. Helicobacter 2014(19), 87-87.
Wang C, Shankar Thangamani, Myunghoo Kim, Bon-Hee Gu, Lee JH, Taparowsky EJ, Kim CH. BATF is required for
normal expression of gut homing receptors by T helper cells in response to retinoic acid. J Exp Med. 2013 March 11;
210 (3): 475-489. (Cited by 44)
Wang C, Lee JH, Kim CH (2012) Optimal Population of FoxP3+ T Cells in Tumors Requires an Antigen Priming-
Dependent Trafficking Receptor Switch. PLoS ONE 7(1): e30793. (Cited by 33)
Wang C, Hillsamer P and Kim CH. Phenotype, effector function and tissue localization of PD-1-expressing human
follicular helper T cell subsets. BMC Immunology 2011 September 13; 12:53. (Cited by 56)
Betz BC, Jordan-Williams KL, Wang C, Kang SG, Liao J, Logan MR, Kim CH, Taparowsky EJ. Batf coordinates
multiple aspects of B and T cell function required for normal antibody responses. J Exp Med. 2010 May 10; 207 (5):
933-42. (Cited by 169)
Wang C, Kang SG, Hogenesch H, Love PE, Kim CH. Retinoic Acid Determines the Precise Tissue Tropism of
Inflammatory Th17 Cells in the Intestine. J Immunol. 2010, 184, 5519 -5526. (Cited by 109)
Kang SG, Wang C, Matsumoto S, Kim CH. High and low vitamin A therapies induce distinct FoxP3+ T cell subsets
and effectively control intestinal inflammation. Gastroenterology. 2009 Oct; 137 (4):1391-402. (Cited by 85)
Lee JH, Wang C, Kim CH. FoxP3+ regulatory T cells restrain splenic extramedullary myelopoiesis via suppression of
hemopoietic cytokine-producing T cells. J Immunol. 2009 Nov 15; 183 (10):6377-86. (Cited by 29)
Lee J, Sun Z, Kim CH. The roles of CCR6 in migration of Th17 cells and regulation of effector
T-cell balance in the gut. Mucosal Immunol. 2009 Mar; 2 (2):173-83. (
Co-first authors, cited by 211)
Qin X.H., Zhang B., Li J., Wang C.W., Yang J.M., Liu J., Zhao D.M. The Sterilization Effect of Glutaraldehyde
Compound Disinfectant on Some Swine Viruses. Acta Lab Anim Sci Sin. 2002, 10 (2): 116-119
Zhang B., Qin X.H., Li J., Wang C.W., Zhao D.M. Test on Efficacy of Complex Disinfectant of Glutaraldehyde and
Quaternary Ammonium Salt. Progress in Veterinary Medicine. 2002, 23 (3): 70-71
Zhang B., Qin X.H., Li J., Yang J.M., Wang C.W., Zhao D.M. Study on Efficacy of the Mixed Disinfectant of
Glutaraldehyde and Quaternary Ammonium Salt against Chicken Pathogen Microorganism. Chin J Lab Anim Sci.
2002,12 (2): 95-98
Wang C.W., Zhao D.M. The Production of Polyclonal Antibody in Laboratory Animals I. Refinement of Immunization
Protocol. Laboratory Animal Science and Administration. 2002,19 (4) : 40-43
Wang C.W., Zhao D.M. The Production of Polyclonal Antibody in Laboratory Animals .Overiew of Adjuvants.
Laboratory Animal Science and Administration. 2003,20(1) : 43-45
Yu S.M., Wang C.W., Zhao D.M., Zhang Q.C., Pei D.Z. Raising and Pathogen Purification of Chinese Experimental
Mini-Pig. Laboratory Animal Science and Administration. 2003,20 (2) : 44-46
Wang C.W., Xu B.R., Zhao D.M., Gao H.D., Li G. Diagnosis and Prevention of Silkychiken Marek's Disease. China
Animal Husbandry & Veterinary Medicine. 2003,30 (3) : 46-47
Wang C.W., Yang J.B., Zhao D.M., Qin X.H. Effects of FK309 Combined with BCG on Sarcoma 180 Cells induced
Tumor. Chinese Journal of Comparative Medicine. 2003, 13(4) :193-196
Yu S.M., Zhao D.M., Li J., Qin X.H., Ning Zh.Y., Yang J.M., Huang L., Wang C.W., Hao J.F. Histopathological
Observation of Bovine Brain Tissue. Chinese Journal of Veterinary Science. 2004, 37 (3): 45-49
Hao J.F., Wu B., Zhao D.M., Ning Zh.Y., Wang C.W., Yu S.M., Su Sh.N. The Study on Pathological Effects of Brain
after High +Gx overload from Spaceship Emergency Return in Rhesus. Space Medicine & Medical Engineering. 2004,
17 (3): 171-175
He C., Zhu H., Wang C.W. Investigation on Immune Response Elicited in Boiler Immunized with Recombinant Major
Outer-Membrance Protein of Chlamydia Psittaci. Journal of China Agricultural University. 2004, 9 (1): 45-48
Wang C.W., He C., WANG L.L., Zhao D.M. Diagnosis of Egg Breeding Chicken Aspergillosis. Chinese Journal of
Veterinary Medicine. 2004, 40 (4): 16-18
Zhou Q.P., Wang C.W. The Discussion of Cured Methods for Canine Organic Phosphor Toxicosis. Chinese Journal of
Veterinary Medicine. 2004, 40 (7): 32-33
Yang C.J., Hao J.F. Wang C.W., Wang J.X., Xu X.F., Zhao D.M. The Pathological Effect of the Chinese Medicine-
Shenqing to Organs of Rat. China Animal Husbandry & Veterinary Medicine. 2004, 31(11) : 39-42
Qin X.H., Kang A.J., Zhao D.M., Yang J.M., Ning Z.Y., Huang L., Wang C.W., Yu S.M., Ma L.Y. Effect of Biological
Dressing on Deep Second Degree Burning Wound Healing of Rat. Chinese Journal of Veterinary Science. 2004, 24(3):
HONORS AND AWARDS
, Murine Chapparvovirus (MuCPV) Assay Development Award, Charles River Laboratories 2019
Bilsland Dissertation Fellowship, Purdue University, 2010-2011
AAI Trainee Abstract Awards, AAI, Seattle, 2009
National Phi Zeta Manuscript Award Nominees, Purdue University, Basic Science, 2009
Ross Fellowship, Purdue University, 2005-2006