scientist resume example with 5+ years of experience
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I am a highly motivated Scientist with a strong academic background and 12 years of experience in the field of neuroscience, cell-molecular biology, and biochemistry. I have excellent project management skills which includes collaborative tasks across cross-discipline teams. I am a reflective, critical thinker, who is able to comprehend complex systems and assist with solutions. My in-depth knowledge of neuroscience, general biology and scientific techniques helps me to successfully run the projects I am in charge of, with remarkable consistency. My interpersonal skills and team working/building capabilities are reflected as I bring new collaborations to my group.
1. Preparation and characterization of mitochondrial DNA-less (ρ0) cells and Parkinson's disease specific trans- mitochondrial hybrid (cybrid) cell lines created from control/patient's mitochondria. 2. Formulation and designing extracellular vesicle-based therapeutics. Characterizing them based on ISEV and standard regulatory guidelines. Determining the critical quClairety attributes, efficacy; quClairety control assessment for evaluating therapeutic property in neurodegenerative and other diseases. 3. Testing Drugs and Biologics and identification of novel neuroprotective and therapeutic target molecules using in vitro neuronal cell culture platforms. 4. Cell culture maintenance and differentiation: Mesenchymal stem cells (MSCs), LUHMES, SH-SY5Y, PC12, Neuro 2a, HEK293T, C6/C8, MDA-MB-231 and 4T1.2-Luc, HS-5, Chondrocyte cell lines 5. Preparation and maintenance of primary cell culture (astrocytes, neurons, endothelial cells, pericytes, neurosphere, neuronal stem cells and mESCs) from neonatal and embryonic rodent brain; setting up transwell co-culture of astrocyte, neuron, and endothelial cells for Blood Brain Barrier in vitro model generation. 6. Operating, handling, and troubleshooting 3D bioprinter for 3D scaffold and cell co-culture, co-printing assay development 7. Transformation and transfection using plasmid vectors; generation of adenovirus particles and transduction in mammClairean cells for gain/ loss of function studies. 8. Rodent stereotaxic surgery for inducing Parkinson's disease and other neurodegenerative disease model in the Substantia Nigra, Striatum, MFB and several other regions of brain (including Cortex, Hippocampus, Dentate Gyrus and Subventricular Zone). 9. Intra cerebral injection of adenovirus particles, plasmid, and drug in rodent brain for gain/ loss of function studies. 10. Characterization for neuronal spine count and morphology from cortical layers by DiI fluorescent staining; Monitoring of neuronal calcium level by spectrofluorimetry and microscopy, using calcium indicator dyes | 11. RNA isolation, cDNA preparation and qPCR. RNA sequencing and analysis of data. 12. Proteomics (Western Blotting, 2D gel electrophoresis followed by MALDI TOF TOF or LC-MS). 13. Mitochondrial function analysis: Genomic and mitochondrial DNA isolation; Mitochondrial electron transport chain complex assays; mitochondrial State 3, 4 respirations; Mitochondrial DNA copy number analysis.; Mitochondrial membrane potential and ROS generation study; Characterization of mitochondrial count, volume, perimeter from Z-stacked confocal images labeled for mitochondria. 14. Cryo-sectioning, Immunocyto/histo-chemistry, Immunofluorescence; Imaging in Olympus FV 1000, Zeiss LSM 780 Upright and Zeiss LSM 780 Inverted Confocal microscopes. Live cell imaging; Image analysis using ImageJ platform. 15. Sub-cellular fractionation; Immunoprecipitation, Chromatin-Immunoprecipitation 16. Analysis of biogenic amines and small peptides by HPLC (ECD) and LC-MS (ESI) 17. Multicolor FACS analysis and working experience with Flow Jo software; ELISA; Cell viability assay. 18. Scanning Electron Microscopy imaging of cells and unicellular organisms in SE, BSE and VP mode after sample preparation using critical point dying and gold sputtering. 19. Establishment of mild traumatic brain injury (TBI) and repetitive mild TBI mice model by controlled cortical impact method. 20. Cancer and metastasis related studies on mice- Generation of breast cancer model in mice by non-surgical mammary fat pad injections of 4T1.2 luc cells; cClaireper measurement of tumors; monitoring metastasis; in vivo and ex vivo IVIS imaging; Trans-cardiac perfusion of rodents. |
Arete Associates Inc – Chantilly, VA,
- Led pathfinding research for discovering extracellular vesicle (EV)-based therapy for neurodegenerative diseases (NDs).
- Simultaneously managed several complex projects, meeting all pertinent milestones.
- Designed projects, planning, and executing experiments; related assay and technology development; determining the QA/QC criteria for the EV based formulation based on standard guidelines
- Utilized my broad and in-depth scientific knowledge towards grant writing, regulatory filings, and patent submissions
- Critical evaluation of clinical data, formulating prospective and retrospective clinical registries and coordinating with medical affairs officer and MSLs on regular meetings
- Hosting educational training and presentation sessions for research associates, scientists, distributor, and sales team
- Mentoring junior team members to effectively analyze data sources and generate reports
- Helping marketing team with scientific communication materials to target specific audience
- Coordinating with CROs for GLP and non-GLP studies for IND applications
- Supporting and advising CMC managers for Biologics therapeutics IND/BLA applications
- Led and successfully completed biodistribution and tolerability study for a key product of the company.
- Brought new collaborations and opened up new research avenues for a couple of products
- Provided competitive intelligence for pharmaceutical products and drug development landscape, to clients, physicians, distributors, and sales team of the company
- Communicated and brought key opinion leaders (KOLs) under our collaboration
New Mexico State University – Las Cruces, NM,
Retroviral-RNA based early detection and transcriptomics analysis of infectious diseases in humans. (Grant funded by: Dept of Commerce, Idaho, USA)
Isolation and identification of retroviral RNA from dried blood spot, library preparation/ RNA sequencing
analyzing, interpreting data and coordinating with PI on grant writing and research plans;
to maintain and work on ethics and compliance with University guidelines for the conduct of research and scholarly activities;
managing the lab in general and principal point of contact for purchasing and ordering
- Wrote and published peer-reviewed articles concerning findings and highlighted possible applications for findings.
- Conducted independent [Type] research and development to attain short and long-term objectives.
- Drafted manuscripts and presented findings at major [Number] [Type] conferences.
New Jersey Institute Of Technology – Newark, NJ,
Volunteered for studying the effect of Nullomer-derived peptides in vivo, in breast cancer-induced mice model. Determined the dose-dependent response of the peptide on mice and established the effective dose which reduces metastasis in the non-surgical mammary fat pad tumor-induced mice model. Characterized the peptide by LC-MS (in continuation). The study though in the preliminary stage seems promising in the field of Cancer and warrant further investigation.
My training includes responsibilities of planning and performing experimental studies using breast cancer induced mice model; maintenance of mice colonies; non surgical mammary fat pad injection; in vivo and ex vivo IVIS imaging; measurement of tumor size and volume; perfusion fixation and multiple organ harvesting; analyzing, interpreting data and coordinating with PI on research plans; performing behavioral tests of the animal after cancer induction and certain drugs/treatments and any other assignments that the principal investigator may request; to comply with University guidelines for the conduct of research and scholarly activities.
- Conducted workshops to engage academic community and generate mission awareness.
- Presented scientific papers at department meetings and symposia.
- Documented changes during experiments, noting unexpected issues arising during research activities.
- Conducted experiments in laboratory environment for product development purposes.
- Maintained accurate records of research findings and provided statistical analysis of data results.
- Leveraged interpersonal and communication skills to mentor PhD, graduate and undergraduate students.
- Collaborated with [Number] multidisciplinary team members to accomplish [Type] research goals.
- Taught [Number] [Subject] courses to students.
George Mason University – Fairfax, VA,
Studied the involvement of mitochondria in Traumatic Brain Injury models. (Grant funded by: NIH, USA)
Duties included performing experimental studies using brain injury model; maintenance of mice colonies; primary cell culture, stem cell culture; histology, immunohistochemistry, and imaging techniques; analyzing, interpreting data and coordinating with PI on research plans; collaborate with PI to write research papers; ordering reagents for lab; performing behavioral tests of the animal after TBI and certain drugs/treatments and any other assignments that the principal investigator may request; to comply with University guidelines for the conduct of research and scholarly activities.
- Contributed to and actively participated in research conception, design and execution to address defined problems.
- Maintained accurate records of research findings and provided statistical analysis of data results.
- Wrote and published peer-reviewed articles concerning findings and highlighted possible applications for findings.
- Conducted research guided by faculty supervisor in accordance with institutional and federal guidelines.
- Received Council of Scientific and Industrial Research National Eligibility Test (CSIR-NET)- Junior Research Fellowship (equivalent to NIH F31)
- Awarded Received
1. International Society for Neurochemistry (ISN) Travel Award for attending 23rd Biennial Meeting.
of ISN at Athens, Greece.
2. 2012- Second prize for poster presentation at Cell Biology and Physiology Research Festival (CBPRF),
Kolkata, India.
3. 2012- First prize for poster presentation at Indian Academy of Neurosciences, Amritsar, India.
4. 2013- International Brain Research Organization (IBRO) travel award and Department of Biotechnology
(DBT, India) travel award for attending 24th Biennial Meeting of ISN at Cancun, Mexico.
5. 2015- First prize for oral presentation at Neurocon- International Conference on "Development,
Degeneration and Regeneration of Neurons: Neurochemistry to Clinical Neurology” Kolkata, India.
6. 2016- International Society for Neurochemistry (ISN) Travel Award for attending 1st Flagship school
organized by ISN and Journal of Neurochemistry (JNC) at Alpbach, Austria
- Elected Captain of Zoology student's counccil
- Received [
1. Prof. S. P. Roychowdry Centenary Medal for securing first rank at the M.Sc. Zoology Exam.
2. 2011- A. B. Mishra Award, for standing first in order of merit at the M.Sc. Zoology Exam.
3. 2011- Prof. Tikaram Sharma Gold Medal, in Molecular and Human Genetics, M.Sc. Zoology.
4. 2011- Shraddha Kumari Srivastav Memorial Award, for MSc Zoology thesis.]
- Awarded
1. 2011- BHU Gold medal for standing first with distinction in M.Sc. Zoology.
- In Boise State University I studied the effect of absent DNA sequences (Nullomer)-derived peptide in breast cancer mouse model
- I established the safety, toxicity, and half-life study of the drug in mice
- I determined the dose dependent response of the peptide on mice and established the effective dose which reduces metastasis in them
- I performed the RNAseq analysis of the excised tumors from the drug and control groups after receiving an institutional grant supported by NIH
- These findings led to two manuscripts under preparation
- (iv) Early HIV RNA detection from DBS
- Later in the same lab, as a postdoc supported by the Idaho Dept of Commerce, I developed protocols and prepared study designs for isolation and detection of HIV RNA from dried blood spot sample, aiming at developing an at-home kit-based test for detecting the viral RNA during early infection
- I wrote the IRB and IBC protocols as a co-PI for this project and was responsible for managing the lab
- Research Support
- Completed Grants
- Funding source: Institutional Development Awards (IDeA) consolidated funding from the National Institute of General Medical Sciences of the National Institutes of Health under Grants #P20GM103408 and P20GM109095, NSF 0923535, MJ Murdock Charitable Trust, NASA EPSCoR NNX10AN29A and Boise State University
- Scientific Equipment Training Grant- Biomolecular Research Center, Boise State University.”
- Role-Awardee
- Support from this grant helped me to carry out experiments at the histology core facility at BSU (October 2019)
- Funding Source: IDeA Network of Biomedical Research Excellence, (Idaho INBRE P20 GM103408), Bioinformatics core; PI- Ken Cornell
- Technology Access Award”
- Role-Awardee
- This grant supported the RNA sequencing studies from the excised tumors of mice (January 2020).
- Linkedin: https://www.linkedin.com/in/Jessica-Claire-phd-31086329/ , USA work status: Eligible to work in the USA. Permanent resident.
- T, C6/C8, MDA-MB-231 and 4T1.2-Luc, HS-5, Chondrocyte cell lines Operating and handling 3D bioprinter for 3D cell culture assay experiments Preparation and maintenance of primary cell culture (astrocytes, neurons, endothelial cells, pericytes, neurosphere, neuronal stem cells and mESCs) from neonatal and embryonic rodent brain; setting up transwell co-culture of astrocyte, neuron, and endothelial cells for Blood Brain Barrier in vitro model generation. Transformation and transfection using plasmid vectors; generation of adenovirus particles and transduction in mammClairean cells for gain/ loss of function studies. Rodent stereotaxic surgery for inducing Parkinson's disease and other neurodegenerative disease model in the Substantia Nigra, Striatum, MFB and several other regions of brain (including Cortex, Hippocampus, Dentate Gyrus and Subventricular Zone). Intra cerebral injection of adenovirus particles, plasmid, and drug in rodent brain for gain/ loss of function studies. Characterization for neuronal spine count and morphology from cortical layers by DiI fluorescent staining; Monitoring of neuronal calcium level by spectrofluorimetry and microscopy, using calcium indicator dyes RNA isolation, cDNA preparation and qPCR. RNA sequencing and analysis of data. Proteomics (Western Blotting, 2D gel electrophoresis followed by MALDI TOF TOF or LC-MS). Mitochondrial function analysis: Genomic and mitochondrial DNA isolation; Mitochondrial electron transport chain complex assays; mitochondrial State 3, 4 respirations; Mitochondrial DNA copy number analysis.; Mitochondrial membrane potential and ROS generation study; Characterization of mitochondrial count, volume, perimeter from Z-stacked confocal images labeled for mitochondria. Cryo-sectioning, Immunocyto/histo-chemistry, Immunofluorescence; Imaging in Olympus FV 1000, Zeiss LSM 780 Upright and Zeiss LSM 780 Inverted Confocal microscopes. Live cell imaging; Image analysis using ImageJ platform. Sub-cellular fractionation; Immunoprecipitation, Chromatin-Immunoprecipitation Analysis of biogenic amines and small peptides by HPLC (ECD) and LC-MS (ESI) Multicolor FACS analysis and working experience with Flow Jo software; ELISA; Cell viability assay. Scanning Electron Microscopy imaging of cells and unicellular organisms in SE, BSE and VP mode after sample preparation using critical point dying and gold sputtering. Establishment of mild traumatic brain injury (TBI) and repetitive mild TBI mice model by controlled cortical impact method. Cancer and metastasis related studies on mice- Generation of breast cancer model in mice by non-surgical mammary fat pad injections of 4T1.2 luc cells; cClaireper measurement of tumors; monitoring metastasis; in vivo and ex vivo IVIS imaging; Trans-cardiac perfusion of rodents. Contributions to Science Parkinson’s disease During my PhD, I studied the motoneuron disorder Parkinson’s disease (PD). Owing to a lack of a reliable model, there is a gap in understanding the actual disease scenario, and hence our group developed and characterized the trans-mitochondrial hybrid (cybrid) cell lines carrying the pathological mitochondria from PD patients (PD cybrids) and matched normal subjects as controls (control cybrids) for the first time in an Indian population. I differentiated these cybrids into dopaminergic neurons and established a glia-cybrid co-culture model that mimics the in vivo PD condition and as a mito-targeted drug testing platform. I conducted the physiological and morphological characterization of these cell lines and performed comparative studies w.r.t. mitochondrial dysfunction observed in the pathophysiological condition. Further, I tested several potential ayurvedic drug candidates with this cybrid model. By comparing the mitochondrial proteome in the cybrids, I identified the role of a novel protein, Prohibitin-1 in relation to PD pathology, which we were first to report in PD. I vClairedated the findings from the cybrid model, in postmortem PD brain samples and in MPTP treated mouse model. The results from my doctoral work provided evidence that the ex-vivo cybrid model recapitulates the mitochondrial dysfunction that occurs in PD patients and the study led to multiple peer reviewed and conference publications which were very well received. Traumatic brain injury I moved to the USA, for my first postdoctoral fellowship supported by a R01 grant, at University of Augusta, followed by University of Pittsburg, where the lab moved later. I studied the role of mitochondria in neurodegeneration after mild traumatic brain injury (TBI). During this time, I gained experience in performing craniotomy and generating TBI model in mice by controlled cortical impact injury technique, handling, breeding, and generating transgenic mice, confocal microscopy, and gained a wider perspective of the extent of neuronal dysfunction after acute and repetitive TBI induction. Moreover, as I utilized my expertise in stereotaxic surgery in rodent model and managing the lab to assist the PI in establishing a new neuroscience laboratory, it was both an exciting and a confidence boosting endeavor.
FDA- Regulatory Education for Industry (REdi) Annual Conference 2021, certificate of attendance CBER, CDER and CDRH tracks.
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Resume Overview
School Attended
- Indian Institute of Chemical Biology
- Banaras Hindu University
Job Titles Held:
- Scientist
- Postdoctoral Researcher
- Visiting Scientist
- Postdoctoral Research Fellow
Degrees
- Ph.D.
- Master of Science
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