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SCIENTIST
Professional Summary

Highly motivated bench scientist with strong molecular skills and expertise in Next Generation Sequencing. Passionate about exploring new technologies. Experienced in lab management and project management. Supportive, results oriented, dedicated to overall lab success and team building.

Skills
  • Proficiency in molecular biology and biochemistry techniques
  • Experience with Next Generation Sequencing instruments and assays
  • Experience with PacBio SMRTbell library prep; operating PacBio RSII and Sequel
  • Knowledge of bioinformatics tools for NGS
  • Excellent analytical and troubleshooting skills
  • Ability to organize, develop, and follow through on tasks and plans
  • Writing Standard Operating Procedures and other technical documentations
  • Excellent interpersonal skills
Work History
Scientist, 06/2018 to Current
Center For Human Immunology, NIAID – Bethesda, MD
  • Work independently and in collaborations with NIAID scientists on a wide range of projects requiring Next Generation Sequencing
  • Test and implement new procedures for NGS preps
  • Present experimental results in a group meetings
  • Implement automation when necessary to improve existing workflows
  • Coordinate purchasing of new equipment and supplies; write justifications and acquisition plans for large purchases.
  • Schedule equipment installations and trainings
  • Manage equipment service contracts

Major contributions:

  • Established fully automated sample processing for RNA-seq
  • Contributed to improvements in sample processing procedures of the NIAID Microbiome core
Laboratory Manager, 12/2010 to 05/2018
USAMRIID, Center For Genome Sciences – Frederick, MD
  • Served as a technical lead; supported implementation of new products and procedures in order to improve lab performance; trained lab personnel and interns
  • Processed samples for sequencing on PacBio RSII and Sequel platforms
  • Managed laboratory equipment; coordinated procurement of new equipment and supplies; guided laboratory infrastructure
  • Ensured compliance of the lab with safety regulations; trained new personnel on lab safety policies
  • Participated in group meetings and presentations; provided assistance in writing reports and manuscripts
    Major contributions:
  • Deep characterization of Rousettus aegyptiacus (Egyptian fruit bat) immune system
  • MHC genotyping of non-human primates using PacBio platform (resulted in award-winning publication)
  • Comparative genomic and transcriptomic studies of pathogenic bacterial strains; sequenced hundreds of bacterial genomes, including novel species
  • Characterization of USAMRIID viral stocks
  • Utility of the microbiome in chemical exposure assessment
  • Characterization of B-cell repertoire in humans and non-human primates during vaccination
Research Associate II, 02/2008 to 11/2010
J. Craig Venter Institute, Genomic Medicine Group – Rockville, MD
  • • Isolated viral fraction from clinical and environmental samples
    • Extracted DNA and RNA; prepared sequencing libraries
    • Prepared viral samples for electron microscopy
    Supported projects that studied:
    • Genomics of multi-drug resistant isolates of Acinetobacter
    • Leptospira genomics and human health
    • Human oral microbiome
    �� Role of HPV in oral cancer
Biotechnologist, 03/2006 to 03/2008
J. Craig Venter Institute, Joint Technology Center – Rockville, MD

• Performed DNA sequencing on ABI 3730xl platform
• Operated and maintained robotic instrumentation and automated DNA sequencers
• Tracked sample status and results using LIMS; performed quality assessment of data and reported process deviations; provided initial troubleshooting solutions

Education
Master of Science: MicrobiologyMoscow M.V. Lomonosov State University - Moscow
Bachelor of Science: BiologyMoscow M.V. Lomonosov State University - Moscow
Publications

1. Stephanie S. Pavlovich, Sean P. Lovett, Galina Koroleva, Jonathan C. Guito, Catherine E. Arnold, Elyse R. Nagle, Kirsten Kulcsar, Albert Lee, Françoise Thibaud-Nissen, Adam J. Hume, Elke Mühlberger, Luke S. Uebelhoer, Jonathan S. Towner, Raul Rabadan, Mariano Sanchez-Lockhart, Thomas B. Kepler, Gustavo Palacios. The Egyptian rousette genome reveals unexpected features of bat antiviral immunity. Cell, 25 Apr 2018, 173(5):1098-1110.e18
DOI: 10.1016/j.cell.2018.03.070 PMID: 29706541
2. Taylor Chance, Jennifer Chua, Ronald G. Toothman, Jason T. Ladner, Jonathan E. Nuss, Jo Lynne Raymond, Fabrice V. Biot, Samandra Demons, Lynda Miller, Stephanie Halasohoris, Sherry Mou, Galina Koroleva, Sean Lovett, Gustavo Palacios, Nicholas J. Vietri, Patricia L. Worsham, Christopher K. Cote, Todd M. Kijek, Joel A. Bozue. A spontaneous mutation in kdsD, a biosynthesis gene for 3 Deoxy-D-manno-Octulosonic Acid, occurred in a ciprofloxacin resistant strain of Francisella tularensis and caused a high level of attenuation in murine models of tularemia. PLoSONE 12(3): e0174106 https://doi.org/10.1371/journal.pone.0174106
3. Apichai Tuanyok, Mark Mayo, Holger Scholz, Carina M. Hall, Christopher J. Allender,a Mirjam Kaestli, Jennifer Ginther, Senanu Spring-Pearson, Molly C. Bollig, Joshua K. Stone, Erik W. Settles, Joseph D. Busch, Lindsay Sidak-Loftis, Jason W. Sahl, Astrid Thomas, Lisa Kreutzer, Enrico Georgi, Jay E. Gee, Richard A. Bowen, Jason T. Ladner, Sean Lovett, Galina Koroleva, Gustavo Palacios, David M. Wagner, Bart J. Currie, Paul Keim. Burkholderia humptydooensis sp. nov., a New Species Related to Burkholderia thailandensis and the Fifth Member of the Burkholderia pseudomallei Complex . Appl Environ Microbiol. 2017 Mar 1; 83(5): e02802-16
4. Sean Lovett, Kitty Chase, Galina Koroleva, Gustavo Palacios, David Rozak, and Jason T. Ladner . Complete Genome Sequence of Pigmentation-Negative Yersinia pestis Strain CadmanGenome Announc. 2016 Sep-Oct; 4(5): e01207-16.
5. Jason W. Sahl, Adam J. Vazquez, Carina M. Hall, Joseph D. Busch, Apichai Tuanyok, Mark Mayo, James M. Schupp, Madeline Lummis, Talima Pearson, Kenzie Shippy, Rebecca E. Colman, Christopher J. Allender, Vanessa Theobald, Derek S. Sarovich, Erin P. Price, Alex Hutcheson, Jonas Korlach, John J. LiPuma, Jason Ladner, Sean Lovett, Galina Koroleva, Gustavo Palacios, Direk Limmathurotsakul, Vanaporn Wuthiekanun, Gumphol Wongsuwan, Bart J. Currie, Paul Keim, David M. Wagne. The Effects of Signal Erosion and Core Genome Reduction on the Identification of Diagnostic Markers mBio. 2016 Sep-Oct; 7(5): e00846-16. Published online 2016 Sep 20. doi: 10.1128/mBio.00846-16
6. Shannon L. Johnson, Kimberly A. Bishop-Lilly, Jason T. Ladner, Hajnalka E. Daligault, Karen W. Davenport, James Jaissle, Kenneth G. Frey, Galina I. Koroleva, David C. Bruce, Susan R. Coyne, Stacey M. Broomall, Po-E Li, Hazuki Teshima, Henry S. Gibbons, Gustavo F. Palacios, C. Nicole Rosenzweig, Cassie L. Redden, Yan Xu, Timothy D. Minogue, Patrick S. Chain. Complete Genome Sequences for 59 Burkholderia Isolates, Both Pathogenic and Near Neighbor Genome Announc. 2015 Mar-Apr; 3(2): e00159-15. Published online 2015 Apr 30. doi: 10.1128/genomeA.00159-15
7. H. E. Daligault, S. L. Johnson, K. W. Davenport, T. D. Minogue, K. A. Bishop-Lilly, S. M. Broomall, D. C. Bruce, S. R. Coyne, K. G. Frey, H. S. Gibbons, J. Jaissle, G. I. Koroleva, J. T. Ladner, C.-C. Lo, C. Munk, M. J. Wolcott, G. F. Palacios, C. L. Redden, C. N. Rosenzweig, M. B. Scholz, P. S. Chain. Draft Genomes for Eight Burkholderia mallei Isolates from Turkey . Genome Announc. 2016 Jan-Feb; 4(1): e01234-15. Published online 2016 Jan 7. doi: 10.1128/genomeA.01234-15
8. Westbrook CJ, Karl JA, Wiseman RW, Mate S,Koroleva G, Garcia K, Sanchez-Lockhart M, O'Connor DH, Palacios G. No assembly required: Full-length MHC class I allele discovery by PacBio circular consensus sequencing. Hum Immunol. 2015 Dec;76(12):891-6. doi: 10.1016/j.humimm.2015.03.022. Epub 2015 May 28.
9. Kugelman JR, Johnston SC, Mulembakani PM, Kisalu N, Lee MS, KorolevaG, McCarthy SE, Gestole MC, Wolfe ND, Fair JN, Schneider BS, Wright LL, Huggins J, Whitehouse CA, Wemakoy EO, Muyembe-Tamfum JJ, Hensley LE, Palacios GF, Rimoin AW. Genomic variability of monkeypox virus among humans, Democratic Republic of the Congo. Emerg Infect Dis. 2014 Feb;20(2):232-9. doi: 10.3201/eid2002.130118.

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Resume Overview

Companies Worked For:

  • Center For Human Immunology, NIAID
  • USAMRIID, Center For Genome Sciences
  • J. Craig Venter Institute, Genomic Medicine Group
  • J. Craig Venter Institute, Joint Technology Center

School Attended

  • Moscow M.V. Lomonosov State University

Job Titles Held:

  • Scientist
  • Laboratory Manager
  • Research Associate II
  • Biotechnologist

Degrees

  • Master of Science : Microbiology
    Bachelor of Science : Biology

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