Life Scientist with a Ph.D. in Biochemistry & Molecular Biology. Well organized, team-oriented, dedicated worker with an enthusiastic approach to challenges of research and with strong communication skills. Extensive experience with current methods of cell biology, biochemistry and immunology. Able to envision new interpretations of experimental findings. Experienced in research, product development and project management. Expertise in Research Experienced in cell biology: cultured adherent cells: endothelial (HUVEC), epithelial (ARPE-19, MDCK, Caco2), fibroblasts (NIH3T3) and neuroblastoma (N2a) cells; cultured muscle myoblasts C2C12 (myocytes) and performed their differentiation into myotubes; cells in suspension: leukemia K-562; preserved stocks; performed very efficient cell transfection; organized inventories. Studied cell receptors (PDGFR, FGFR, EGFR, TGFR, TNFR, VEGFR,Toll-like & integrin receptors), and their ligands as well as the mechanisms involved in signal transduction cascades (second messenger's generation, receptor's clustering, cascades of protein phosphorylation). Signal transduction pathways in physiological conditions and in various diseases (celiac disease, Age Related Macular Degeneration). Developed new as well as adapted and optimized established techniques (Cell based ELISA for CD46, CD55, CD59; Fluorescence based permeability assay for differentiated Caco2 monolayer treated with cytokines; quantitative ELISA assay for prion protein in blood; quantitative assay for fibrinogen degradation products in blood). Investigated RhoA GTPase in its role in cell adhesion, cytoskeleton organization and assembly of actin stress fibers upon integrin receptor's engagement in fibronectin/tissue transglutaminase (extracellular matrix) interaction; suppression of src tyrosine kinase inhibitory effect on RhoGTPase Activating Protein (p Have broad knowledge in biological sciences exercised in numerous projects in both academia and industry, leading to high level of confidence in work Highly adaptable and goal oriented, multitasking work style
Research Specialist - Staff Member January 2009 to January 2011Johns Hopkins University School of Med, Wilmer Eye Institute
was found responsible for this RhoA activation and enhanced cellular adhesion Analyzed transcriptional profiles in oxidative (Cigarette Smoke Extract treatment) and inflammatory (cytokine treatment) stress responses of Retinal Pigment Epithelial cells in vitro.
Tested Reactive Oxygen Species, lipid peroxidation, complement factors C3 & C5 by qRT-PCR and biochemically Performed animal studies: genotyping, animal surgery (isolation of particular tissues from mouse, collecting internal organ's tissues, collecting blood from eye and heart, intraperitoneal and intravenous injections.
Expert in Immunohistochemistry - cryosectioning, sectioning of paraffin embedded tissues; Confocal, electron and optical microscopy Served as both an independent researcher and as a team member; supervised technicians and students; acquired, interpreted and presented research results Published 11 papers 16 communications on international conferences Leadership and management Highly effective trouble-shutter: helped young scientists achieve reproducible, reliable results Ordered laboratory supplies and reagents; providing summaries of weekly laboratory expenses Kept inventory levels of delivered supplies and reagents, proper storage Managed tissue culture facility, cultivation of various cell lines and cryopreserving cell stocks; Participated in animal facility management: breeding animals Prepared clear and scientifically informative presentations for every lab meeting and for bigger audiences Maintained records of instrument's and equipment validation and service as well as records of attendance at regulatory/lab safety training sessions Trained new lab members in rules of biohazard and chemical waste disposal Wrote manuscripts for publications, protocols, SOPS & templates for new as well as commonly used, optimized techniques Trained new employees, fellows and students in biochemical, cellular and molecular biology and other laboratory techniques; helping them in calculations and data processing and analysis Replaced commonly used, expensive and time consuming techniques by less expensive and more time effective ones; my greatest accomplishment was improving research efficiency of the entire laboratory by developing, evaluating and introducing to the lab some (frequently used) costs effective and time saving methods; it helped to improve the research outcome and quality for every lab member.
Senior Research Associate January 2008 to January 2009ALBA Therapeutics, Inc Baltimore
Postdoctoral Research Fellow January 2004 to January 2006Johns Hopkins University School of Medicine
January 2000 to January 2004American Red Cross － Rockville, MD
Holland Laboratory for the Biomedical.
Sciences, Departments of Biochemistry and of Plasma Derivatives; Research Fellow II.
Department of Pathology
Ph.D : 1987
Biochemistry, Medical University in Lodz, (Lodz, Poland) USA evaluated
M.S : Molecular Biology, 1979University of LodzMolecular Biology Lodz, Poland) USA evaluated
07. 1996University of Bern, Theodor Kocher Institute － Bern, Switzerland
1994UFR des Sciences Pharmaceutiques et Biologiques, Laboratoire d'Hematologie － Paris, France