Experienced professional seeking a responsible and challenging
position in innovative research.
Master of Science: BiotechnologyBangalore UniversityIndiaBiotechnology
Bachelor of Science: MicrobiologyBangalore UniversityIndiaMicrobiology
03/2009 to Current
Studying chromosomal alterations involved in Fanconi Anemia, a very rare, recessive
genetic disorder debilitating developmental abnormalities, and other life threatening
In order to better understand the genetics of FA, I am currently screening
large number of FA patient cell lines to sequence the DNA for indels, mutations by DNA
sequencing, coupled with other techniques like Comparative Genomic hybridization and Next
08/2007 to 09/2008
Research SpecialistEMORY UNIVERSITY Atlanta Georgia
10/2005 to 06/2006
Research AssistantWinship Cancer Institute － Bangalore, India
Primarily worked on molecular targets considered to be potent drugs like TNF-.
inducing ligand (TRAIL), which is able to induce apoptosis, by binding to
cell membrane receptors.
Recombinant forms of these ligands are capable to potentiate the
effect of chemotherapeutic drugs in vitro and in vivo in the animal model.
drugs on Human lung cancer cell lines invitro
for their cytotoxicity and studied effect of these drugs on cell lines by detecting
different protein expression levels.
Study the effect of fusion proteins on cell receptors: Construction, expression and
characterization of fusion proteins.
JNCASR (JN Center for Advanced Scientific Research.
Research and Development Assistant
Malarial Parasitology lab)
Exploring the metabolic pathway in apicomplexan Plasmodium falciparum parasite.
the role of FabH protein in Plasmodium falciparum by extraction and purification of
recombinant FabH Protein (B keto acyl synthase III, a protein of Fatty acid synthase).
Extract and purify plasmid DNA, Genomic DNA, RNA from cell lines, Perform PCR, RT PCR,
RTqPCR and DNA sequencing on ABI 3730xl DNA analyzer.
Vector construction, Primer designing, Restriction digestion, ligations, Gene cloning,
Transfections with plasmid DNA, GFP Vectors, Stable and transient transfection of protein
Protein purification and Western analysis:
Study protein expression by SDS PAGE and Western analysis from total cell lysate.
expression, extraction and Purification of His tagged proteins from E.coli culture in
large amount by affinity chromatography.
Detect protein expression levels to study drug effects by treating mammalian cell lines
Experience working with both adherent and non-.
CHO cells for stable and transient transfection and protein expression Bacterial cell
culture for Cloning, transformation.
U2OS cells for GFP and FLAG tagged DNA transfections for RNA and western analysis.
Mammalian cell lines like Fibroblasts and LCL for total genomic Isolation and sequencing.
Mammalian lung cancer cell lines for drug screening.
Plasmodium falciparum culture maintenance in Human RBC's under invitro condition.
In vivo procedures:
Plasmodium bergii culture maintenance in vivo in mice.
Harvesting blood from mice by
Isolation of T-.
lymphocytes from mice spleen.
Intraperitoneal injection, Head and neck orthotropic injection.
Monitored and maintained a highly organized laboratory environment.
inventories, accounts, records, and computer databases.
Demonstrated and trained others in
the proper operation of laboratory techniques and procedures.
Reviewed literature for
related research developments.
Assisted supervisor in literature, proofreading of papers.
Have complete knowledge and understanding of Laboratory ethics and Lab bio safety rules
Distinguished Performance award 2010.
Honored as BEST STUDENT OVERALL 2003-.
Honored as BEST DISCIPLINARY STUDENT 2004-.
Incidental medical information in whole-.
Solomon BD, Hadley DW, Pineda-.
Alvarez DE; NISC Comparative Sequencing Program, Kamat
A, Teer JK, Cherukuri PF, Hansen NF, Cruz P, Young AC, Berkman BE, Chandrasekharappa SC,
Mullikin JC.2012 Jun
De novo deletion of chromosome 20q13.33 in a patient with tracheo-.
cardiac defects and genitourinary anomalies implicates GTPBP5 as a candidate gene.