Highly motivated researcher with more than 5 years of lab-based experience in biochemistry and microbiology and with more than two years of working experience in food research laboratories Over 1 year of experience in developing SOPs for product (food) manufacturing and of quality control of product Hands on expertise in microbiological, chemical, physical and functional analysis of food samples (technical expertise have been listed below) Sound knowledge of food manufacturing process, GMP, GLP, HACCP, ISO standards, SOPs, SSOPs, sanitation program and quality assurance gained from course works in Food safety and quality assurance program at University of Guelph, Canada Proficient in Microsoft office and experience with inventory management Strong interpersonal skills developed from working and interacting with students, scientists, professors and through working with interdisciplinary groups and projects Excellent oral and written communication skills including the ability to organize and record data, and write and present scientific papers/technical reports
Gel filtration, affinity chromatography, HPLC, GC, TLC, ultra-filtration & dialysis, spectrophotometry, enzyme assays (amylase, xylanase, glucose oxidase), total starch content, ash content, SDS PAGE, western blotting, silver staining, sample preparation and analysis for mass spectroscopy, MALDI-TOF, and scanning electron microscopy (SEM), titration, Bradford, Lowry and DUMUS for total protein content, gluten content, extensibility, rheology, viscosity, co-immunoprecipitation (IP/CHIP), immunostaining
Microbiological techniques: Media preparation, aseptic technique, culture, growth, enumeration, enrichment, plating (differential and selective media), bacterial quantification using culture methods, spectrophotometry, ATP meter, real time PCR, antimicrobial assay, gram and fluorescent staining
Molecular and cell biology: Plasmid and genomic DNA/ RNA/mRNA isolation, PCR, real time PCR, restriction digestion, gel electrophoresis, cloning, reporter assay, 16s rRNA Sequencing, cloning, polysome gradient ultracentrifugation, fluorescent In situ hybridization (FISH), radiolabeling
Statistical analysis/ Computer skills: ANOVA, Pearson Correlation, Duncan's multi-range test; MS Office (word, excel, power point, outlook); software like DNASTAR, Image J, Primer 3, BLAST
Grain Biopolymer Research Lab, University of GuelphMay 2015 to November 2015Research Associate
Worked for an industry to improve the quality of the raw material by various enzymatic modification in order to enhance the quality of the final product Carried out composition and functional property analysis of flour samples: moisture, total protein, and starch content; particle size, color, water absorption and stability, viscoelastic properties, viscosity and rheology Regularly calibrated lab equipment (texture analyzer, RVA, balances, incubator, pH meter) Analyzed texture of different food products like croissants, bread, muffins, cookies Developed SOPs for the production of the industrial baking product, and for test methods to monitor the quality of products at various intermediary stages of production (Dough consistency and extensibility) and of final products (measuring volume and texture) Regularly participated in sensory analysis conducted in the department as well as in the lab for new food products including croissant, bread, and muffin Assisted in protein extraction, quantification by SDS PAGE and HPLC from different types of flour Documented, reviewed/analyzed data generated from various quality control (QC) process, analyzed the root cause of deviation in the process, prepared technical reports and presented reports of work progress Collaborated with other members of the lab and other departments for sample analysis and with industry partners for material supplies.
University of GuelphMay 2013 to April 2015Graduate Research Assistant
Developed a standard antimicrobial (chemical) treatment method against pathogenic bacteria including E.
coli O157:H7, Salmonella and Listeria monocytogenes on seeds (critical control point in sprout production) for sprout industry Performed routine microbiological testing of seeds, sprouts, and sprout-irrigation water for detection and quantification of contaminant by culture methods, and carried out quantitative and statistical analysis and graphing (Microsoft excel) of the data to look for trends Researched and proposed rapid detection/quantification method (likely to reduce testing time to approximately 4h compared to 48 h in case of culture method) for bacteria occurring in low numbers that can be optimized and validated as quality control testing for sprouts Assisted in microbiological analysis (bacteria, yeast, and mold) of food samples including fresh produce, sausages, dairy products from industries Trained students in lab safety practices, aseptic techniques, generating growth curve using spectrophotometry and CFU count, environmental sampling of food contact and non-food contact surfaces including rapid (ATP testing), culture methods (petrifilms, RODAC plates, selective media culture, total count), gram staining and PCR.
University of GuelphNovember 2006 to April 2013
Worked on multiple projects emphasizing on protein-protein and RNA-protein interaction.
Routinely involved in extraction as well as affinity purification of proteins from complexes, determining total protein content and characterizing by SDS PAGE, silver or commassie staining, western blotting and by MS (approximately 10-14 gel electrophoresis/week) Extracted DNA and RNA from infected/transfected cells and carried out quantitative analysis using PCR and real time PCR; stained cells with fluorescent dyes and carried out quantitative determination of dead/necrotic cells Worked with different cell lines, sub-cultured and cryopreserved cells, counted cells using cell counter/hemocytometer and checked morphology, viability and involved in troubleshooting contamination of cell lines with bacteria, yeast, mold, and mycoplasma by fluorescence/immunostaining, microscopy, PCR, sequencing Optimized methodologies for isolating proteins from aggregates by co-immunoprecipitation and for localizing protein in vivo by immunostaining Regularly interacted with other departments for analysis of samples using mass spectrometry, flow cytometry, microscopy, sequencing and with company representatives for purchasing products/equipment/technical experts for troubleshooting Trained new lab entrants with lab safety practices, culture methodologies, SDS PAGE, western blotting, immunoprecipitation, cloning, PCR, and qPCR; maintained lab equipment Organized monthly lab meetings for work distribution and work progress discussion, meetings with company sales representatives, and training for new lab members Managed multiple concurrent projects each with their own deadlines and general laboratory operation including lab inventory management, ordering supplies, and cataloguing chemicals and their MSDS using spreadsheets.
Indian Institute of Technology (IIT) DelhiDecember 2000 to September 2006New Delhi, India
Isolated bacteria from roots and soil and identified nitrogen fixing Rhizobium by culture method, biochemical tests, and sequencing.
Developed and optimized method for identifying/detecting flavonoids in root/seed extract of rice infected with bacteria by HPLC, HPLC-MS, and for identifying sugars by GC.
Worked in a project that involved collaboration of IIT/Indian agricultural research institute/Laboratory of Tropical and Mediterranean Symbioses, France.
Worked as an exchange student at Laboratory of Tropical and Mediterranean Symbioses, France for 6 months.
Dept. of Molecular and Cellular Biology
Education and Training
University of Guelph2015MSc. Food Safety and Quality Assurance, Food ScienceOntario, Canada
Indian Institute of TechnologyPhDDelhi, India
University of HyderabadMSc: BiotechnologyHyderabad, IndiaBiotechnology
OFPA (Ontario Food Protection Agency)
*ASCB (American Society of Cell Biology)
Bhattacharjee RB, Bag J (2012) Depletion of nuclear poly (A) binding protein PABPN1 produces a compensatory response by cytoplasmic PABPC4 and PABPC5 in cultured human cells. PLoS One. 2012; 7(12): e53036. Bhattacharjee RB, Zannat T, Bag J (2012) Expression of the polyalanine expansion mutant of nuclear Poly (A) binding protein induces apoptosis via the p53 pathway. Cell Biol Int.36:697-704 Bhattacharjee RB, Jourand P, Chaintreuil C, Dreyfus B, Singh A, Mukhopadhyay SN (2012) Indole acetic acid and ACC deaminase-producing Rhizobium leguminosarum bv. trifolii SN10 promote rice growth and in the process undergo colonization and chemotaxis. Biol Fertil Soils 48:173-182 Bag J, Wang Q, Bhattacharjee RB (2012) Nuclear poly (A)-binding protein and Oculopharyngeal muscular dystrophy. Muscular Dystrophy, Chapter 2, 25 Zannat T, Bhattacharjee RB, Bag J (2011) Depletion of cellular poly (A) binding protein prevent protein synthesis and results in apoptosis in HeLa cells. BBRC 409, 171-176 Zannat T, Bhattacharjee RB, Bag J (2011) PABP depletion leads to p53 dependant apoptosis in HEK and HeLa cell. BBRC 408, 375-381 Bag J, Bhattacharjee RB (2010) Multiple levels of post-transcriptional control of expression of the poly (A)-binding protein. RNA Biology 7:5-12 Ma S*, Bhattacharjee RB*, Bag J (2009) Translation of poly (A)-binding protein is up regulated during recovery from heat shock in HeLa cells. FEBS Journal, 276:552-70 (*equal contribution) Bhattacharjee RB; Singh A, Mukhopadhyay SN (2008) Use of nitrogen-fixing bacteria as biofertilizer for non-legumes, Appl Microbiol Biotechnol 80:199-209
Citizenship: Canada (Eligible for TN Work Authorization)
Sponsorship not required
Citizenship: Canada (Eligible for TN Work Authorization)
Sponsorship not required
aseptic techniques, aseptic technique, ATP, biochemistry, biology, color, content, dialysis, DNA, Food Safety, functional, GC, HPLC, Image, inventory management, IP, irrigation, laser, meetings, Microsoft excel, excel, exchange, MS Office, outlook, power point, word, MSc, PAGE, PCR, peak, progress, purchasing, quality, quality control, Quality Assurance, quantitative analysis, real time, reporter, research, safety, sales, sample preparation, scanning, spreadsheets, Statistical analysis, troubleshooting, UV, western blotting, composition