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Postdoctoral Scientist/Project Manager Resume Example

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POSTDOCTORAL SCIENTIST/PROJECT MANAGER
Accomplishments
  • SeaVuria is a Seattle-based NGO focused on connecting students in Vuria hills in Taita Kenya and Washington State through transformative experiences that inspire them to love and use science and technology (STEM) to make a difference in their lives, and beyond: www.seavuria.org.
  • One of the original Board Members.
  • Responsible for developing policy, strategy, and vision.
  • Formulating policies, writing budgets, letters of engagement with similar non-profits and NGOs.
  • Assist with fundraising efforts among key donors.
  • We serve 11 schools in Seattle and 12 Schools in Taita-Kenya.
  • Member, Board of Directors, Christ the King School (Seattle) 2011 - 2012 Responsible for developing policy, strategy, and vision.
  • Formulating policies, writing budgets.
  • Participated in guiding the school through the accreditation process.
  • Approval of school calendar and syllabus.
  • Serve as the interface between the school and Christ the King Church.
Professional Summary

Highly organized [Job Title] proficient at developing, qualifying and validating Immunoassays and performing ELISAs.

Research Biologist offering expertise in animal experiments, molecular biology and laboratory management experiences.

Research Scientist with more than [Number] years experience in field, office and lab settings with a strong focus on [Topic].

Scientist experienced in establishing investigative tasks and motivating staff to attain goals. Offers knowledge of laboratory equipment, safety practices and biohazard disposal.

Biologist seeks to improve the scientific community by performing accurate research and complying with all laboratory safety protocol and standards.

Highly skilled [Job Title] with [Number] years in radiological threat agents and emerging diseases. Advanced in surveillance, clinical research and laboratory testing. Research Biologist practiced in cell-based assay development, molecular biology and genetics, as well as drafting corresponding technical and research documents. Research Biologist practiced in cell-based assay development, molecular biology and genetics, as well as drafting corresponding technical and research documents.

Skills
  • Proficient in PCR
  • Published researcher
  • Skilled in genotyping
  • Protein isolation
  • Transcription profiling
  • Statistical analysis
  • Gene expression
  • Cytoplasmic protein extraction
  • ELISA assay
  • UV and Vis Spectrometry
  • Immunohistochemistry assay knowledge
  • Published author
  • Organization of Biological Field Stations
  • Standard methods
  • American Society for Microbiology
  • Certified in Applied Biology
  • RNA isolation knowledge
Work History
09/2013 to Current
Postdoctoral Scientist/Project ManagerJohn Aitchison Lab/Seattle Biomedical Research Institute Seattle BioMed – Seattle, WA
  • Able to write, review and update SOP's, technical documents, application notes and test methods.
  • Record data in logbooks and laboratory notebooks per GLP.
  • Maintains strictest standards of hygiene and safety.
  • Adept at safe Radiochemical usage and handling.
  • Instrumentation: UV-visible absorption and fluorescence spectroscopy.
  • Atomic absorption.
  • Confocal, bright field and fluorescence microscopy.
  • Flow cytometer Akata chromatography systems Surface Plasmon resonance spectroscopy (BiaCore).
  • Fluorometer/luminometer and plate reading assays.
  • Cell Culture: Maintenance of Plasmodium in-vitro cell culture.
  • Perform in-vitro drug assays using the cultures, determining the mode of action for combinatory drugs.
  • Generation of transgenic lines and maintenance of multiple cell lines in cell culture.
  • Analysis of integration and generation of cloned lines.
  • Pull down assay/immunoprecipitation of protein complexes using tagged transgenic lines.
  • Protein-protein interaction studies and characterization of this interaction using Surface Plasmon resonance technique (BiaCore).
  • Immuno Fluorescence Assays (IFA) and confocal microscopy.
  • Isolation and assays involving peripheral blood mononuclear cells (PBMC).
  • Mouse Model: Generation of tagged genes.
  • Knockout genes in mouse models.
  • Transfection of transgenic genes.
  • Analysis of integration and generation of cloned lines.
  • Test for essentiality of genes in mouse models.
  • Immuno Fluorescence Assays (IFA) and confocal microscopy of mouse model tagged genes.
  • Trained small animal handler.
  • Molecular Biology and Microbiology: Excellent knowledge of PCR based techniques.
  • Gene and recombinant DNA cloning, site directed mutagenesis PCR Cloning full-length genes by PCR, sub cloning to expression vectors.
  • DNA sequencing analysis, In-vitro transcription/translation, RNA extraction.
  • Southern blot, northern blot.
  • Plasmid preparation, restriction digests analysis.
  • General microbiology and media preparation.
  • Cell culture techniques; culture maintenance, aseptic techniques.
  • Interpreted research findings and summarized data into reports.
  • Encouraged cooperative management strategies.
  • Conducted basic and applied research on [Area of study].
  • Facilitated research into genetic causes of disease.
  • Worked with several teams on [Area of study] research.
  • Communicated research results to individuals in the industry, government and general public.
  • Designed and optimized assays.

05/2012 to 09/2013
Postdoctoral Scientist/Project ManagerMichal Fried Lab/Seattle Biomedical Research Institute Seattle BioMed – Seattle, WA
  • Conducting research on Malaria immunology and pathogenesis with special emphasis on biomarker discovery and pregnancy malaria initiative.
  • Recruited from Malcolm Gardner's lab to join Michal Fried lab and lead the pregnancy malaria biomarker discovery research initiative.
  • Isolation, culture, and characterization of primary immune cells from blood Investigating pregnancy malaria macrophage secrotome.
  • Pregnancy malaria biomarkers discovery and validation from placenta serum extracts.
  • Mass spectroscopy analysis of Pregnancy malaria placenta serum extracts Investigating the metabolic maps derived from malaria and pregnancy malaria field samples.
  • Investigating the causative factors behind maternal anaemia, low birth weight, pre-eclampsia and other complications associated with pregnancy malaria.
  • Assisted in manufacturing and testing of [Assay type] assays.
  • Diagnosed certain autoimmune and infectious diseases.
  • Kept accurate databases of specimens collected and stored in repository.
  • Collected and processed specimens for clinical protocols.
  • Designed and optimized assays.
  • Successfully completed transcription profiling experiments.
  • Acquired all import permits needed for international colleagues.
  • Regularly communicated with scientific research teams from [Country], [Country] and [Country].
  • Viewed and compared genomic content.
  • Validated [Number] cancer targets in animal models.
  • Worked with several teams on [Area of study] research.
  • Facilitated research into genetic causes of disease.
  • Interpreted research findings and summarized data into reports.
  • Encouraged cooperative management strategies.
  • Collected and analyzed biological data about relationships between organisms and their environment.
  • Conducted basic and applied research on [Area of study].

07/2009 to 05/2012
Postdoctoral Scientist/Project ManagerThe Bottom Line
  • Identified and validated pregnancy malaria biomarkers using placenta samples from pregnancy malaria patients.
  • Scientific Legacy First lab to perform mass spectroscopic studies using placenta samples from pregnancy Malaria patients.
  • Designed and optimized the method to investigate the secrotome of macrophages isolated from pregnancy Malaria patient's placenta samples.
  • Identified Pregnancy Malaria biomarkers and validated them in clinical samples Significant contributions Using a systems biology approach identified and validated pregnancy malaria biomarkers (2 manuscripts in preparation).
  • Manage and perform experiments in a BSL2 environment in accordance with GLP/GCP.

04/2005 to 11/2008
Doctoral Scientist/PhD Candidate/Graduate InstructorMalcolm Gardner Lab/Seattle Biomedical Research Institute Seattle BioMed – Seattle, WA
  • Conducting research on aminoacylation pathways in the Apicoplast of Plasmodium falciparum as potential drug targets as well as evaluating them as potential vaccine candidates Recruited after my Ph.D.
  • studies in Germany Expression, purification and kinetic characterization of enzyme drug targets Determine potency of synthetic compounds against enzyme drug targets Responsible for Independently planning, designing, executing, trouble-shooting, and interpretation of all experiments in the lab which involved biochemical, analytical, microbiology, molecular biology, cell culture and mouse model experiments.
  • Design, develop and implement, biophysical, biochemical and cell-based assay for the new targets.
  • Develop and validate novel in-vitro assays to support high throughput screening of compounds and lead compound identification Utilize my expertise to characterize protein-protein interactions and signaling pathways for the identified targets (e.g.
  • Biacore, Proximity Ligation Assay, Flow Cytometry, Immunofluorescence-based cell imaging, co-IPs, cDNA expression cloning).
  • Elucidate a complete structure-function relationship through in-silico modelling and crystallisation of these enzymes.
  • Managing all the projects being undertaken by our group and making sure all experiments adhere to GLP's and institutional safety regulation.
  • Responsible for acquisition, analysis and interpretation of data, generation of statistical graphs and visualizations, charts, tables, writing of reports and manuscripts.
  • Responsible for collaborative research with internal or external research groups.
  • The Bottom Line
  • Conducted basic and applied research on [Area of study].
  • Collected and analyzed biological data about relationships between organisms and their environment.
  • Encouraged cooperative management strategies.
  • Interpreted research findings and summarized data into reports.
  • Facilitated research into genetic causes of disease.
  • Extracted DNA and genotype samples using SNP technology.
  • Proctored scientific meetings.
  • Worked with several teams on [Area of study] research.
  • Validated [Number] cancer targets in animal models.
  • Attended industry conferences to broaden knowledge.
  • Communicated research results to individuals in the industry, government and general public.
  • Managed overall laboratory functions.
  • Cared for and studied rodents and rabbits.
  • Scheduled and trained technical staff members.
  • Complied with Good Laboratory Practices and Title 21 Code of Federal Regulations Part 11.
  • Regularly communicated with scientific research teams from [Country], [Country] and [Country].
  • Acquired all import permits needed for international colleagues.
  • Successfully completed transcription profiling experiments.
  • Improved calibrator performance and production by [Action].
  • Designed and optimized assays.
  • Managed the sampling and characterization of new transgenic crops.
  • Ordered laboratory equipment and supplies.
  • Diagnosed certain autoimmune and infectious diseases.
  • Assisted in manufacturing and testing of [Assay type] assays.
  • Assisted in manufacturing and testing of [Assay type] assays.

01/2002 to 01/2004
LecturerEgerton University

01/2002 to 01/2003
Research AssistantInstitute of Primate Research

01/2001 to 01/2004
Research AssistantEgerton University Njoro – Egerton University Njoro

Education
Nov. 2008
Ph.D.: Biochemistry, Molecular biology, Parasitology
Justus-Liebig-University - Giessen, Germany
GPA: Magna Cum Laude
June 2004
Master of Science: Chemistry
Egerton University Noro - Kenya.
Chemistry

Nov. 2001
Bachelor of Science: Chemistry/Biochemistry and Molecular Biology
Egerton University Njoro - Kenya
Chemistry, Biochemistry and Molecular Biology
Affiliations
American Society of Tropical Medicine and Hygiene (ASTMH): Member. American Chemical Society (ACS): Member. American Society for Cell Biology (ASCB): Member. American Society for Quality (ASQ): Member. International Society for Pharmaceutical Engineering (ISPE): Member. Regulatory Affairs Professional Society (RAPS). Awards and Honours American Society of Cell Biology (Minority Affairs Committee) Travel grant 2015 Deutscher Akademischer Austausch Dienst (DAAD) Ph.D. Fellowship. 2005 - 2008 Deutscher Akademischer Austausch Dienst (DAAD) German language study grant 2004 - 2005
Publications
Mailu B M, Ling L, Arthur J, Nelson T M, Ramasamy G, Fritz-Wolf K, Becker K, Gardner M J. (2015). Plasmodium Apicoplast Gln-tRNAGln biosynthesis utilizes a unique GatAB amidotransferase essential for erythrocytic stage parasites. J. Biol.Chem Dec 4; 290(49): 29629-41 Jortzik E, Zocher K, Isernhagen A, Mailu B M, Rahlfs S, Viola G, Wittlin S, Hunt N H, Ihmels H, Becker K. (2015). Acridizinium derivatives have a strong antimalarial activity and inhibit indoleamine 2,3 dioxygenase. Antimicrobial Agents and Chemotherapy Oct 12; 60(1): 115-25. Mailu B M, Ramasamy G, Mudeppa D, Ling L, Lindner S, DeRocha A, Rathod P K, Kappe S H, Gardner M J. (2013). Expression and characterization of apicoplast glutamyl-tRNA synthetase, The first enzyme in an indirect aminoacylation pathway in P. falciparum. J. Biol.Chem Nov 8; 288(45): 32539-52. Jortzik E*, Mailu B M*, Preuss J, Fischer M, Bode L, Rahlfs S, Becker K. (2011). Glucose 6-phosphate dehydrogenase 6-phosphogluconolactonase from Plasmodium falciparum. Biochem J. Jun 15; 436(3): 641-50. *contributed equally to the work. Austin CJD*, Mailu B M*, Maghzal G J, Sanchez-Perez A, Rahlfs S, Zocher K, Arthur J, Becker K, Stocker R, Hunt N H, Ball H J. (2010). Biochemical characteristics and inhibitor selectivity of mouse indoleamine 2,3-dioxygenase-2. Amino Acids. Jul; 39(2):565-78. *contributed equally to the work. Sturm N, Jortzik E, Mailu B M, Koncarevic S, Deponte S, Rahlfs S, Forchhammer K, Becker K. (2009). Identification of proteins targeted by the thioredoxin superfamily in Plasmodium falciparum. PLoS Pathogens. Apr; 5 (4):e1000383. Rahlfs S, Koncarevic S, Iozef R, Mailu B M, Savvides S N, Schirmer R H, Becker K. (2009). Myristoylated adenylate kinase 2 of Plasmodium falciparum forms a heterodimer with myristoyl transferase. Mol. Biochem. Parasitol. 163 (2) 77-84. Mailu B M, (2009). Plasmodium falciparum Glucose 6-Phosphate Dehydrogenase-6-Phosphogluconolactonase. Characterisation of Redox-Related Networks as Contribution to the Development of Novel Intervention Strategies. Shaker Verlag GmbH Aachen. ISBN 978-3-8322-8134-2. Buchholz K, Mailu B M, Schirmer R H, Becker K. (2007). Structure based drug development against malaria. Frontiers in Drug Design and Discovery, 3: 225-255. PERIODICALS Mailu B M, Becker K. (2007). Warum haben Kinder in Africa so oft Fieber? "e.velop" 52 (Internet Magazine der Bundesregierung zur Entwicklungspolitic). CONFERENCES & SCIENTIFIC MEETINGS Mailu B M, Rahlfs S, Austin CJD, Hunt N H, Becker K. (2006). Further optimisation of the heterologous overexpression of mouse indoleamine 2, 3-dioxygenase. 22 Jahrestagung der Deutschen Gesellschaft für Parasitologie, Wien, February 2006. Austin CJD, Mailu B M, Sanchez-Perez A, McQuillan J, Astelbauer F, Rahlfs S, Aurther J,Becker K, Ball H J, Hunt N H. (2007). Indoleamine 2, 3-dioxygenase like protein 1 (INDOL 1). A novel heme containing tryptophan catabolising enzyme. IUPAC Conference, Turin Italy, July 2007. Mailu B M, Hiller N, Fritz-Wolf K, Rahlfs S, Becker K. (2007). Interference with redox active proteins as a basis for the design of antimalarial drugs. Drug Development Seminar, Tübingen, April 2007. Mailu B M, Rahlfs S, Becker K. (2008). Glucose 6-phosphate dehydrogenase-6-phosphogluconolactonase from Plasmodium falciparum. Jahrestagung der Deutschen Gesellschaft für Parasitologie, Hamburg, March 2008. Mailu B M, Peterson M, Arthur J, Ramasamy G, Mudeppa D, Rathod P, Gardner M J. (2010). Expression and kinetic characterisation of non discriminating Plasmodium falciparum apicoplast glutamyl-tRNA synthetase (PfGluRS). American Society of Tropical Medicine and Hygiene 59th Annual Meeting, Atlanta Georgia November 2010
Certifications
Additional Information
  • "John's outstanding client focus has led our USA Bank branch to outperform others and surpass annual profit goals." - Henry Adams, Financial Manager, USA Bank
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Resume Overview

Companies Worked For:

  • John Aitchison Lab/Seattle Biomedical Research Institute Seattle BioMed
  • Michal Fried Lab/Seattle Biomedical Research Institute Seattle BioMed
  • The Bottom Line
  • Malcolm Gardner Lab/Seattle Biomedical Research Institute Seattle BioMed
  • Egerton University
  • Institute of Primate Research
  • Egerton University Njoro

School Attended

  • Justus-Liebig-University
  • Egerton University Noro
  • Egerton University Njoro

Job Titles Held:

  • Postdoctoral Scientist/Project Manager
  • Doctoral Scientist/PhD Candidate/Graduate Instructor
  • Lecturer
  • Research Assistant

Degrees

  • Ph.D. : Biochemistry, Molecular biology, Parasitology
    Master of Science : Chemistry
    Bachelor of Science : Chemistry/Biochemistry and Molecular Biology

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