|Molecular biology and gene manipulation techniques: PCR, RT-PCR, conventional recombinant DNA cloning, high-throughput cloning (Gateway, PIPE), site-directed and random DNA mutagenesis, cDNA libraries construction and screening, plasmid and bacmids extraction and purification, RNA extraction, purification and in vitro transcription Cell cultures and protein expression: Gene delivery techniques: transformation, viral transfection, electroporation and lipofection. Propagating bacterial (E. coli), yeast, insect and mammalian cell lines (CHO, HEK|
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