Goal-driven research scientist in the field of molecular and cellular biology.
* Passionate and collaborative scientist with proven record of project design and execution.
* Team-oriented mentor with strong communication and organizational skills.
* Good understanding and proficient hands-on techniques in molecular and cellular biological research and in vitro/cell based assay system development. Molecular Biology
Molecular cloning, in vitro mRNA synthesis, Real-time PCR (qPCR), Western blotting
Cell Biology
Rodent primary neuron culture, Mammalian cell culture, Stable cell line construction, transfection/infection, Gene knockdown/knockout by shRNA system, Fluorescent microscopy
Biochemistry
Enzyme assays, DNA/RNA gel electrophoresis, Luciferase reporter assays, ELISA, Immunoprecipitation
Protein Characterization
Protein expression/purification from mammalian cell line, Protein expression/purification from bacteria/yeast/insect cell, FPLC chromatography