Experienced in the synthesis of peptides containing both natural and non-natural amino acids as well as purification and subsequent binding analysis of peptide products. I am versed in various binding analysis techniques including: fluorescence polarization, isothermal titration calorimetry, and surface plasmon resonance.
Solid Phase Peptide Synthesis
Chemical Modification of Peptides
High Performance Liquid Chromatography
Surface Plasmon Resonance
Isothermal Titration Calorimetry
Recombinant Protein Expression
08/2013 - Current
Geisel School of Medicine - Hanover, NHPhD Candidate
11/2011 - 05/2013
Saint Michael's College - Colchester, VTPeer TutorOrganic Chemistry
06/2011 - 08/2011
Vermont's Genetic Network - Colchester, VermontSummer Research
Development of an isotopically labeled hydrophobicity tag for enhanced electrospray ionization mass spectrometry analysis.".
01/2010 - 05/2013
Saint Michael's College - Colchester, VermontBiochemistry Lab Preparation
Prepared reagents and tested lab procedures
Geisel School of Medicine, DartmouthHanover, NHExperimental TherapeuticsLab of Dr. Mark Spaller: Synthesis and characterization of PDZ domain targeting peptides
Saint Michael's CollegeColchester, VTBachelor of Science: BiochemistryGraduated with Honors
Heidi M Chapman, Katherine L Schutt, Emily M Dieter, and Shane M Lamos. Relative quantification of biomarkers using mixed-isotope labeling coupled with MS. Bioanalysis. 2012;4:2525-2541.
Knafo S, Sanchez-Puelles C, Palomer E, Delgado I, Draffin JE, Mingo J, Wahle T, Kaleka K, Mou L, Pereda-Perez I, Klosi E, Faber EB, Chapman HM, Lozano-Montes L, Ortega-Molina A, Ordez-Gutirrez L, Wandosell F, Via J, Dotti CG, Hall RA, Pulido R, Gerges NZ, Chan AM, Spaller MR, Serrano M, Venero C, Esteban JA. PTEN recruitment controls synaptic and cognitive function in Alzheimer's models. Nat Neurosci. 2016;19:443-53
Characterization of a PDZ domain containing protein, GIPC,
and its binding partners. Presented at Gordon Research Conference Peptides,
Chemistry and Biology of, 2016.
Got Starch? Decoding the Carbon partitioning defective4 (Cpd4) mutant gene in maize. Presented at Undergraduate Research Symposium, University of Missouri June 2012. National Maize Genetics Conference Spring, 2013.
Development of an isotopically labeled hydrophobicity tag for enhanced electrospray ionization mass spectrometry analysis. Presented at Dean's Reception 2012 and Sigma Xi induction 2012.
Synthesis, characterization, and optimization of PDZ domain binding peptide sequences
Protein expression and purification
Training of new lab members
Beta Beta Beta Honor's Society- Vice President 2011-2012, President 2012-2013
Sigma Xi Honor's Society
Dean's List recipient Fall 2009- 2013.
Knight's At the Round Table Award 2010-2013 (presented to Varsity athletes who maintain a cumulative GPA of 3.5 or above).
Outstanding Biochemistry Student Award, 2013
ACS Top Organic Chemistry Senior Award, 2013
Saint Michael's College Varsity Swim Team: 2009-2013