Ph D Candidate Resume Example

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Jessica Claire
  • , , 100 Montgomery St. 10th Floor
  • Home: (555) 432-1000
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Professional Summary

Enthusiastic [Job Title] eager to contribute to team success through hard work, attention to detail and excellent organizational skills. Clear understanding of [Task] and [Task] and training in [Skill]. Motivated to learn, grow and excel in [Industry].

  • Individualized support
  • Documentation proficiency
  • Modern teaching methods
  • Group Instruction
Work History
Ph.D. Candidate, 08/2017 to Current
CelerionPhoenix, AZ,

Cellular senescence (CS) is an irreversible cell cycle arrest elicited in response to endogenous and exogenous stresses including DNA damage, telomere dysfunction and oncogene activation to limit the replication of old or damaged cells. Senescence has been linked to diverse biological processes such as tissue repair, embryogenesis, tumor suppression and organismal ageing. Senescent cells undergo several phenotypic alterations such as metabolic reprogramming, chromatin rearrangement, and secrete a combination of factors, resulting in a ‘senescence associated secretory phenotype’. With time, these cells accumulate in the organism contributing to tissue dysfunction and chronic age-related pathologies including cancer. Studies suggest that clearance of senescent cells attenuates age-related deterioration by improving tissue repair capacity and alleviating chronic, low-grade inflammation. However, selective and effective clearance of senescent cells of different cell types as well as different types of senescence (replicative, DNA-damage induced) is challenging due to lack of known biomarkers. As a result, there is a growing interest in finding novel senescent markers.

Phage display is a powerful technology that leverages libraries of compounds with different binding capabilities for the characterization of molecular diversity at the cell surface level, in vitro or in vivo. The technology has been widely used to screen, identify, and isolate clinically relevant and unique ligand-receptor pairs for a variety of cells and tissues that can serve as targets for disease diagnosis, profiling, imaging, and therapy. In this study, using the biopanning and rapid analysis of selective interactive ligands (BRASIL) method, we screened a phage peptide library (CX8C, where ‘C’ represents cysteine residues and ‘X’ represents any amino acid) on human senescent dermal fibroblasts and selected potential candidates based on repeating peptide motifs.

Using cell-based phage-binding assays, we further compared the binding of the selected clones to senescent cells relative to the insertless control phage, Fd-tet. We observed that some of these clones exhibited a more pronounced binding to senescent cells compared to the insertless control phage, Fd-tet. Our data suggest that the preferential binding may be due to the interaction of the expressed peptide with a putative receptor present on the surface of the cells. To ensure that this interaction is due to the nature of the peptide (ligand) expressed by the phage, phage internalization and receptor identification studies will be performed. Identification of such a unique ligand-receptor combination could be of clinical relevance and may provide novel avenues for ligand-directed delivery of imaging agents, therapeutic genes, or drugs to senescent cells in ageing and ageing-associated diseases.

Pertinent skills: Peptide phage display technology, fluorescent microscopy, standard PCR, cDNA synthesis, RT-PCR, agarose gel electrophoresis, SDS-PAGE, 2D-gel electrophoresis, western blotting, cell culture techniques, plasmid isolation and purification, restriction digestion, transformation,

Research Specialist, 03/2016 to 04/2017
IntersectionDallas, TX,

Worked with a team of bioengineers and neurosurgeons to develop a novel drug delivery technique for spinal tumors via magnetic guidance of intrathecally administered doxorubicin-loaded magnetic nanoparticles.

Validated the concept of intrathecal magnetic drug targeting via site-specific magnetic localization of intrathecally administered nanoparticles in Sprague Dawley rats using external magnets.

Pertinent skills: Synthesis of magnetic nanoparticles and drug-loaded magnetic nanoparticles, cell culture and maintenance of rat and human glioblastoma cell lines, in vitro cytotoxicity assays, intrathecal administration of nanoparticles in Sprague Dawley rats, spinal cord extraction, and histological analysis.

Trainee, 11/2011 to 03/2012
King George’s Medical UniversityCity, STATE,
  • Shadowed a team of clinicians and researchers in the department of microbiology to understand standard diagnostic laboratory operations.
  • Assisted senior team members in the bacteriology and virology divisions with medical testing of patient samples.

Pertinent skills: ELISA, nucleic acid extraction, agarose gel electrophoresis, standard PCR, real-time PCR (Sybr Green and Taqman), Gram’s staining, bacterial culture techniques, antibiotic susceptibility tests, documentation of patient records, data analysis.

Project Trainee, 01/2011 to 05/2011
Central Drug Research InstituteCity, STATE,

Completed a short-term project to identify immuno-dominant proteins of Candida albicans using polyclonal sera from mice & humans. Common immunodominant antigens were identified by patient and mice sera that may be of importance for the development of diagnostic techniques for candidiasis.

Pertinent skills: Maintenance of C. albicans (ATCC 10231) cultures, protein isolation (bead beating method), protein dialysis and lyophilization, protein quantification (lowry method), SDS PAGE, Coomassie and silver staining, ELISA, 2D- gel electrophoresis, Western blot.

PhD: Biomedical Sciences, Molecular biology, Genetics & Cancer, Expected in 07/2022
Rutgers University - Newark, NJ,
Master of Science: Molecular And Cell Biology, Expected in 12/2015
Illinois Institute of Technology - Chicago, IL,
Master of Science: Microbiology, Expected in 07/2011
University of Lucknow - Lucknow, India,
Bachelor of Science: Biology And Chemistry, Expected in 04/2009
Isabella Thoburn College, University of Lucknow - Lucknow, India,

Rutgers Center of Organizational Leadership

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School Attended
  • Rutgers University
  • Illinois Institute of Technology
  • University of Lucknow
  • Isabella Thoburn College, University of Lucknow
Job Titles Held:
  • Ph.D. Candidate
  • Research Specialist
  • Trainee
  • Project Trainee
  • PhD
  • Master of Science
  • Master of Science
  • Bachelor of Science