Field Applications Specialist Resume Example
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FIELD APPLICATIONS SPECIALIST
Professional Summary
A innovative and detail oriented scientific professional with extensive experience in developing and managing the implementation of novel technologies for the analysis of DNA and RNA in R&D and sequencing production environments. R&D researcher with demonstrated knowledge in molecular biology, protein chemistry, and assay development. Specialty areas include comprehensive knowledge of Sanger and next generation sequencing technologies, genomic data analysis, project management, optimization and automation of next generation sequencing processes, assay development, and team leadership and mentoring.
Skills
| Development of Automated Processes for NGS
Experience with Fluidigm BioMark and RainDance Thunderstorm and RainDrop Technologies R&D and Technology Transfer Expertise in Genomic Analysis Development of DNA/RNA extraction methods | High-Throughput Assay Development
Project Management Sequencing Data Anaysis Team Leadership and Mentoring Comprehensive knowledge of molecular biology and protien chemistry Personable communicator |
Work History
01/2016 to Current
Senior Field Application ScientistHamilton Company – Marin City , CA
- Responsible for the pre and post-sales support including on site customer product/application demonstrations, presenting the company's technology through seminars and workshops, training customers on as well as helping with the analysis of data generated with the company products.
- Tracked and recorded all relevant information in SFDC related to all technical inquiries from customers, documented complaints and worked with other departments within ArcherDx to resolve the customer issues.
- Provided feedback to the sales and marketing group on current and emerging market opportunities for oncology products.
- Communicating customers needs into oncology product development group to help develop a product that meets the customer's requirements.
- Worked with several KOLs in presenting their results using Archer products for oncology at prominent journals and conferences.
- Worked with other Applications Specialists to maintain an FAQs and Knowledge Base as it relates to the technical aspects of ArcherDX products.
- Participated in workshops and trade shows to help find new sales leads.
- Training and mentoring new members of the FAS team.
- Management of the Certified Service Provider program for ArcherDX. This includes identifying and vetting potential CSP sites, training FAS team on the CSP program and supporting the FAS team during the certification process.
09/2014 to 01/2016
Field Applications SpecialistDanaher – Romulus , MI
- Responsible
for the pre and post-sales support including on site customer product/application
demonstrations, presenting the company's technology
through seminars and workshops, training customers on as well as helping with the analysis of
data generated with the company products.
- Tracked and recording all relevant
information in SFDC related to all technical inquiries from customers, documented complaints and worked
with other departments within Enzymatics to resolve the customer issues.
- Provided feedback to the sales and marketing
group on current and emerging market opportunities.
- Worked
with other Applications Specialists to maintain an FAQs and Knowledge base as
it relates to the technical aspects of Enzymatics
products.
- Participated in workshops and trade shows to help find new sales leads.
01/2014 to 09/2014
Field Application ScientistApex Systems – Aurora , IL
- Responsible for delivering both pre and post sales support for the company's target enrichment platforms, real time qPCR platform, and the Apollo robot and library construction reagents for next generation sequencing platforms for the eastern half of the United States. This support was for both research and clinical customer accounts.
- Provided to customers onsite training of WaferGen Biosystems platforms, troubleshooting customer issues through telephone, email communications and onsite visits, and organized instrument demonstrations at the request of the sales account owner.
- Provided to customers onsite training of WaferGen Biosystems platforms, troubleshooting customer issues through telephone, email communications and onsite visits, and organized instrument demonstrations at the request of the sales account owner.
- Delivered feedback to the Sales and Marketing group members as to users needs and wants in expanding the use of WaferGen's technologies.
- Worked with sales account representative and the marketing group to develop custom programs on the Apollo robot for NGS library construction.
- Worked with the R&D group in development of NGS library prep reagents for DNA and RNA library construction in both research and clinical laboratories.
- Maintained all customer communications and visits in SFDC. Documented customer complaints and worked with R&D and Marketing to resolve the issue in a timely manner.
- Participated in trade shows and delivered scientific talks at trade shows and customer sites. Worked to help fine other sales prospects.
- Maintained my knowledge in the target enrichment, real time qPCR applications, and NGS library and sequencing methodologies in order to help WaferGen develop new products for this marketplace.
04/2008 to 01/2014
Scientist/Project Manager, New Technology Development GroupBritish American Tobacco – Brainerd , MN
- Led a small group to evaluate, develop, implement, and document sample submission requirements as well as library preparation and quantitation protocols for next generation sequencing platforms in a core facility. Platforms include 454 FLX+, IlluminaHiSeq and PGM
- Project Manager for the NIIAD biobanking program of Flu Viral HA and NA segments. Set timelines for synthesis of these antigenic viral segments, sequencing, and delivery of over 2500 separate sets for researchers to use.
- Project Manager for the de novo sequencing of the Atlantic Salmon and Conus Consors (cone snail) for two international sequencing projects. Set up the timelines and mile markers for sequencing, assembly and delivery of data to the international consortiums.
- Project Manager for the automation of the Core Sequencing Lab at JCVI. Was responsible for the development of timelines and milestones during this transformation. Worked with outside vendors to obtain reagents and competitive pricing.
10/2002 to 04/2008
Scientist, Synthetic BiologyJ. Craig Venter Institute – City , STATE
- Led a small R & D group (3 junior lab members) in assembling the Mycoplasma genetalium chromosome from oligonucleotides. Assigned lab tasks, advised the design of laboratory experiments, and set timelines for completion of work.
- Developed methods for using Capillary Electrophoresis (CE) to purify oligonucleotides and assess their purity before entering the chromosome assembly process.
- Preformed Sanger sequencing and data analysis in order to identify and confirm correctly assembled 5 Kb cassettes of the Mycoplasma genetalium chromosome (ABI3100 and ABI3730 sequencers).
- Used recombination to join correctly assembled 5Kb cassettes into 10, 20, and 40 Kb segments.
- Used molecular biology techniques to attempt to remove Type II restriction enzyme encoding sequences from the Mycoplasma genetalium chromosome.
- Led a small group (2 junior lab members) in DNA extraction and library construction from organisms collected on filters during a survey of the world's oceans.
- Presented research to the scientific community at meetings and in peer reviewed journals.
03/2000 to 10/2002
Scientist, DNA RescourcesCelera Genomics Corp – City , STATE
- Involved in the development of methods to construct small, medium, and large insert libraries for whole genome shotgun sequencing allowing for successful sequencing the Human genome.
- Developed and documented quality control assays for library construction.
- Responsible for Sanger library construction for the sequencing projects.
- Developed methods for the synthesis and expression of pseudo genes from oligonucleotides. Confirmed synthesis via Sanger sequencing.
12/1999 to 03/2000
Scientist, Quality ControlCelera Genomics Corp – City , STATE
- Developed, documented, and implemented assays to help maintain or improve data quality generated by Sanger sequencing in a GLP environment.
- Developed and used methods such as LC-MS (ESI), RP-HPLC, pH and UV-VIS spectroscopy to assess sequencing primer length and quality and antibiotic stability.
- Developed quality control assays for LB agar stability
08/1998 to 12/1999
Research Associate IV, Biochemistry GroupLife Technologies Inc – City , STATE
- Senior member of the Biochemistry group in a GMP manufacturing facility.
- Purified restriction enzymes, fluorescent dyes, and EGF using IEX, affinity, IMAC, RP-HPLC, and SEC for products used by life science researchers.
- Used SDS-PAGE, agarose gel electrophoresis, and radioactive/functional assays to assess product purity and activity.
- Worked in BSL1, BSL2, and BSL3 laboratory environments.
- Assigned other group members activities in conjunction with the director of Biochemistry group.
09/1996 to 05/1998
Senior Research Associate, Macromolecular Structure GroupABL-BRP, NCI-FCRDC - MD – City , STATE
- Primarily responsible for the purification of proteins of interest for the Macromolecular Structure group for both protein structure and function studies using NMR and crystallography.
- Responsible for the set up the protein purification laboratory including the procurement of equipment.
- Produced manuscripts describing research findings in peer reviewed journals. Presented research to the scientific community internally and at conferences.
- Used Western blots, SDS-PAGE, UV-Vis spectroscopy, CD-ORD, NMR and crystallography to characterize the proteins.
- Purified STAT-4, Leptin, TEV Protease.
Education
B. A: Chemistry and BiologyLuther College - City, State
Larsen Presidential Scholar from Luther College
Larsen Presidential Scholar from Luther College
M. S: Medicinal and Natural Products ChemistryUniversity of Iowa, College of Pharmacy - City, State
Predoctoral Fellowship from the University of Iowa Center for Biocatalysis and Bioprocessing
Predoctoral Fellowship from the University of Iowa Center for Biocatalysis and Bioprocessing
Publications
Brauer
A, Kurz A, Stockwell T, Baden-Tillson
H,
Heidler J, Wittig I, Kauferstein S, Mebs D, Stocklin R, and
Remm M, " The
Mitrochondrial genome of the venomous cone snail Conus consors," PLoS One,
2012;
7(12)e51528. PMID23235512
Tim Stockwell, Holly Baden-Tillson, Philippe Favreau, Dietrich Mebs, Fredric Ducanncel, and Reto Stocklin, "Sequencing the genome of the Conus consors: preliminary results," Advances and New Technologies in Toxicology, Meeting on Toxicology, 2010-SEFT Editions 11-16.
Maroti G, Tong Y, Yooseph S, Baden-Tillson H, Smith HO, Kovacs KL, Frazier M, Venter JC, Xu Q, “Discovery if (NiFe) hydrogenase genes in meagenomic DNA cloning and heterlogous expression in Thiocapsa roseopersicina,” Appl Environ Microbiol, 2009 Sep; (18): 5821-30.
Daniel G Gibson, Gwynedd A Benders, Cynthia Andrews-Pfannkoch, Evgeniya A Denisova, Holly Baden-Tillson, Jayshree Zaveri, Timothy B Stockwell, Anushka Brownley, David W Thomas, Mikkel A Algire, Chuck Merryman, Lei Young, Vladimir N Noskov, John I Glass, J Craig Venter, Clyde A Hutchison III, Hamilton O Smith, “Complete Chemical Synthesis, Assembly, and cloning of a Mycoplasma genetalium Genome,” Science, 2008 Feb29; 319(5867):1215-20.
Douglas B Rusch, Aaron L Halpern, Granger Sutton, Karla B Heidelberg, Shannon Williamson, Shibu Yooseph, Dongying Wu, Jonathan A Eisen, Jeff M Hoffman, Karin Remmington, Karen Beeson, Bao Tran, Hamilton Smith, Holly Baden-Tillson, Clare Stewart, Joyce Thorpe, Jason Freeman, Cynthia Andrews-Pfannkoch, Joseph E Venter, Kelvin Li, Saul Kravitz, John F Heildelberg, Terry Utterback, Yu-Hui Rogers, Louis Falcon, Valeria Souza, German Bonilla-Rosso, Luis E Eguiarte, David M Karl, Shubha Sathyendrath, Trevor Platt, Eldredge Bermingham, Victor Gallardo, Giselle Tamayo-Castillo, Michael R Ferrari, Robert L. Strassberg, Kenneth Nealson, Robert Friedman, Marvin Frazier, J Craig Venter, “The Sorcerer II Global Ocean Sampling Expedition: Northwest Atlantic through Eastern Tropical Pacific,” PLoS Biol, 2007 Mar 13, 5 (3):e77 17355176.
Venter JC, Remington K, Heildelberg JF, Halpern AL, Rusch D, Eisen JA, Wu D, Paulsen I, Nelson KE, Nelson W, Fouts DE, Levy S, Knapp AH, Lomus MW, Nealson K, White O, Peterson J, Hoffman J, Parsons R, Baden-Tillson H, Pfannkoch C, Rogers YH, and Smith HO, “Environmental genome shotgun sequencing of the Sargasso Sea,” Science, 2004, Apr. 2; 304(5667): 66-74.
Gibbs RA, Weinstock GM, Holt RA, Adams MD, Amanatides PG, Baden-Tillson H, Barnstead M, Pfannkoch C, Venter JC, et al., “Genome Sequence of the Brown Norway Rat yields insights into mammalian evolution,” Nature, 2004, Apr. 1; 428(6982): 493-521.
Venter JC, Adams MD, Myers EW, Smith HO, Baden H, et. al., “The genome sequence of the malaria mosquito Anopheles gambiae,” Science, 2002, Oct. 4; 298(5591):129-49.
Aparicio S, Chapman J, Stupka E, Putnam N, Chai JM, Dehal P, Christoffels A, Rash S, Hoon S, Smit AF, Sollewijn Gelpke MD, Roach J, Oh T, Ho IY, Wong M, Detter C, Verhoef F, Predki P, Tay A, Lucas S, Richardson P, Smith SF, Clark MS, Edwards YJ, Dogget N, Zharkikh A, Tavtigian SV, Pruss D, Barnstead M, Evans C, Baden H, Powell J, Glusman G, Rowen L, Hood L, Tan YH, Elgar G, Hawkins T, Venkatesh B, Rokhsar D, and Brenner S, “Whole-Genome Shotgun Assembly and Analysis of the Genome of Fugu rubripes,” Science, 2002, Aug. 23; 297(5585):1301-10.
Venter JC, Adams MD, Myers EW, Smith HO, Baden H, et. al., “The sequence of the Human Genome,” Science, 2001, Feb. 16; 291(5507):1304-51.
H.A. Baden, R.B. Kapust, S.P. Sarma, R.A. Byrd, and D.S. Waugh, “The Amino Terminal Domain of STAT4: Overproduction, Purification, and Biophysical Characterization,” J. Biol. Chem., 1998, Jul 3, 273(27) 17109-14.
Tim Stockwell, Holly Baden-Tillson, Philippe Favreau, Dietrich Mebs, Fredric Ducanncel, and Reto Stocklin, "Sequencing the genome of the Conus consors: preliminary results," Advances and New Technologies in Toxicology, Meeting on Toxicology, 2010-SEFT Editions 11-16.
Maroti G, Tong Y, Yooseph S, Baden-Tillson H, Smith HO, Kovacs KL, Frazier M, Venter JC, Xu Q, “Discovery if (NiFe) hydrogenase genes in meagenomic DNA cloning and heterlogous expression in Thiocapsa roseopersicina,” Appl Environ Microbiol, 2009 Sep; (18): 5821-30.
Daniel G Gibson, Gwynedd A Benders, Cynthia Andrews-Pfannkoch, Evgeniya A Denisova, Holly Baden-Tillson, Jayshree Zaveri, Timothy B Stockwell, Anushka Brownley, David W Thomas, Mikkel A Algire, Chuck Merryman, Lei Young, Vladimir N Noskov, John I Glass, J Craig Venter, Clyde A Hutchison III, Hamilton O Smith, “Complete Chemical Synthesis, Assembly, and cloning of a Mycoplasma genetalium Genome,” Science, 2008 Feb29; 319(5867):1215-20.
Douglas B Rusch, Aaron L Halpern, Granger Sutton, Karla B Heidelberg, Shannon Williamson, Shibu Yooseph, Dongying Wu, Jonathan A Eisen, Jeff M Hoffman, Karin Remmington, Karen Beeson, Bao Tran, Hamilton Smith, Holly Baden-Tillson, Clare Stewart, Joyce Thorpe, Jason Freeman, Cynthia Andrews-Pfannkoch, Joseph E Venter, Kelvin Li, Saul Kravitz, John F Heildelberg, Terry Utterback, Yu-Hui Rogers, Louis Falcon, Valeria Souza, German Bonilla-Rosso, Luis E Eguiarte, David M Karl, Shubha Sathyendrath, Trevor Platt, Eldredge Bermingham, Victor Gallardo, Giselle Tamayo-Castillo, Michael R Ferrari, Robert L. Strassberg, Kenneth Nealson, Robert Friedman, Marvin Frazier, J Craig Venter, “The Sorcerer II Global Ocean Sampling Expedition: Northwest Atlantic through Eastern Tropical Pacific,” PLoS Biol, 2007 Mar 13, 5 (3):e77 17355176.
Venter JC, Remington K, Heildelberg JF, Halpern AL, Rusch D, Eisen JA, Wu D, Paulsen I, Nelson KE, Nelson W, Fouts DE, Levy S, Knapp AH, Lomus MW, Nealson K, White O, Peterson J, Hoffman J, Parsons R, Baden-Tillson H, Pfannkoch C, Rogers YH, and Smith HO, “Environmental genome shotgun sequencing of the Sargasso Sea,” Science, 2004, Apr. 2; 304(5667): 66-74.
Gibbs RA, Weinstock GM, Holt RA, Adams MD, Amanatides PG, Baden-Tillson H, Barnstead M, Pfannkoch C, Venter JC, et al., “Genome Sequence of the Brown Norway Rat yields insights into mammalian evolution,” Nature, 2004, Apr. 1; 428(6982): 493-521.
Venter JC, Adams MD, Myers EW, Smith HO, Baden H, et. al., “The genome sequence of the malaria mosquito Anopheles gambiae,” Science, 2002, Oct. 4; 298(5591):129-49.
Aparicio S, Chapman J, Stupka E, Putnam N, Chai JM, Dehal P, Christoffels A, Rash S, Hoon S, Smit AF, Sollewijn Gelpke MD, Roach J, Oh T, Ho IY, Wong M, Detter C, Verhoef F, Predki P, Tay A, Lucas S, Richardson P, Smith SF, Clark MS, Edwards YJ, Dogget N, Zharkikh A, Tavtigian SV, Pruss D, Barnstead M, Evans C, Baden H, Powell J, Glusman G, Rowen L, Hood L, Tan YH, Elgar G, Hawkins T, Venkatesh B, Rokhsar D, and Brenner S, “Whole-Genome Shotgun Assembly and Analysis of the Genome of Fugu rubripes,” Science, 2002, Aug. 23; 297(5585):1301-10.
Venter JC, Adams MD, Myers EW, Smith HO, Baden H, et. al., “The sequence of the Human Genome,” Science, 2001, Feb. 16; 291(5507):1304-51.
H.A. Baden, R.B. Kapust, S.P. Sarma, R.A. Byrd, and D.S. Waugh, “The Amino Terminal Domain of STAT4: Overproduction, Purification, and Biophysical Characterization,” J. Biol. Chem., 1998, Jul 3, 273(27) 17109-14.
Presentations (Posters)
Venter JC, Remington K, Hoffman J, Baden-Tillson H, Pfannkoch C, and Smith
HO, “ Environmental Genome Shotgun Sequencing of the Sargasso Sea,” DOE
Genomics: GTL Workshop II, Washington, DC, February 29-March 2, 2004.
Glass JI, Alperovich N, Assad-Garcia N, Baden-Tillson H, Khouri H, Lewis M, Nierman WC, Nelson WC, Pfannkoch C, Remington K, Yooseph S, Smith HO, and Venter JC, “Estimation of the Minimal Mycoplasma Gene Set Using Global Transposon Mutagenesis and Comparative Genomics,” DOE Genomics: GTL Contractor-Grantee Workshop II, Washington, DC, February 29-March 2, 2004.
R.B. Kapust, H.A. Baden, and D.S. Waugh – “Advances in TEV Protease Engineering: Improved Yield, Enhanced Stability, and Unconventional Applications,” 13th Annual Advanced Bioscience Laboratory Retreat, Stanton, VA, October 27-29, 1997.
H.A. Baden, R.B. Kapust, S.P. Sarma, A. Zdanov, A. Wlodawer, R.A Byrd, and D.S. Waugh - “The Amino Terminal Domain of STAT4: Overproduction, Purification, and Structural Characterization,” 13th Annual Advanced Bioscience Laboratory Retreat, Stanton, VA, October 27-29, 1997.
Glass JI, Alperovich N, Assad-Garcia N, Baden-Tillson H, Khouri H, Lewis M, Nierman WC, Nelson WC, Pfannkoch C, Remington K, Yooseph S, Smith HO, and Venter JC, “Estimation of the Minimal Mycoplasma Gene Set Using Global Transposon Mutagenesis and Comparative Genomics,” DOE Genomics: GTL Contractor-Grantee Workshop II, Washington, DC, February 29-March 2, 2004.
R.B. Kapust, H.A. Baden, and D.S. Waugh – “Advances in TEV Protease Engineering: Improved Yield, Enhanced Stability, and Unconventional Applications,” 13th Annual Advanced Bioscience Laboratory Retreat, Stanton, VA, October 27-29, 1997.
H.A. Baden, R.B. Kapust, S.P. Sarma, A. Zdanov, A. Wlodawer, R.A Byrd, and D.S. Waugh - “The Amino Terminal Domain of STAT4: Overproduction, Purification, and Structural Characterization,” 13th Annual Advanced Bioscience Laboratory Retreat, Stanton, VA, October 27-29, 1997.
References Available on Request
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Resume Overview
School Attended
- Luther College
- University of Iowa, College of Pharmacy
Job Titles Held:
- Senior Field Application Scientist
- Field Applications Specialist
- Field Application Scientist
- Scientist/Project Manager, New Technology Development Group
- Scientist, Synthetic Biology
- Scientist, DNA Rescources
- Scientist, Quality Control
- Research Associate IV, Biochemistry Group
- Senior Research Associate, Macromolecular Structure Group
Degrees
- B. A : Chemistry and Biology
M. S : Medicinal and Natural Products Chemistry
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