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Experimental chemist Resume Example

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EXPERIMENTAL CHEMIST
Summary
Scientist with a deep expertise in genetics, molecular biology and biochemistry. Works in the field of Metabolic syndrome, cancer research, neuroscience and transgenesis . Uses state-of-the-art reverse genetic approaches to generate mouse models for the basic research. Responsible to publishing results in prestigious scientific journals. Highly appreciated by collaborators for his drive to succeed. Excellent attention to detail Management and analytical skills Flexibility Creativity Complete the tasks to the agreed deadlines Ability to resolve complicated problems Multitask Organization and prioritization of responsibilities Excellent communication skills Team Player
Highlights
  • Project planning,development and finalization
  • Collaboration
  • Presentation
  • Supervision technical staff
  • Obesity
  • Chronic stress
  • Diabetes
  • Brown fat function
  • Liver cancer
  • Pancreas cancer
  • Neuroscience
  • Puberty onset regulation
  • Inflammation
  • Drug addiction
  • Neurodegenerative diseases
  • Cell assay
  • Eukaryotic cell cultures
  • Molecular biology/ Biochemistry techniques
  • Microscopy
  • Imaging
  • Transgenesis: Mice
  • Plants
  • Microorganisms
  • Lentiviral transgenesis
  • Cre/LoxP
  • RNAi
  • TALENs
  • IT skills: Vector NTI 11
  • pDRAW32
  • Ensembl
  • Microsoft Office
  • Prism
  • Photoshop
  • Bioinformatics: R
Accomplishments
  • Memberships in the Society of Neuroscience (Sfn) 2009 Presentations Heidelberg International Symposium on Late Diabetic Complications 27-28.10.2014 & Annual Junior ICEMED Meeting, Leipzig 2014 & DKFZ-ZMBH Alliance Retreat 2014 Kloster Schöntal & Translating Diabetes, EMBL Heidelberg 2014 & Workshop Innovative Mouse Models, Leiden 2013 2011 2007 & DKFZ-ZMBH Alliance Retreat 2013 Kloster Schöntal & ICEMED Annual Meeting 2013 & Metabolism; From Signaling to Diseases DKFZ-ZMBH Alliance Forum 2012 & 7th Forum of European Neuroscience Amsterdam 2010 & Society for Neuroscience Annual Meeting Chicago 2009.
Experience
01/2011 to CurrentGerman Cancer Research Center DKFZ
  • Senior scientist in the Joint Research Division: Molecular Metabolic Control (A170) Projects on brown fat function under high glucocorticoid stress conditions.
  • Mouse model with a floxed allele of Rip140 gene & Macrophage Cre Bac mouse line & Two knock-in mouse models that will be used in a complementary approach to address the role of "brite" fat cells & Neuron specific Dox- inducible Cre Bac mouse line (a joint effort with GeneBridges GmbH, Heidelberg and Prof.
  • A.
  • Herbison, University of Otago, N Zealand) & Coordinates mouse knock-in projects between TaconicArtemis GmbH and the department Conducts in vivo experiments to investigate the harmful effect of excess glucocorticoids on the brown fat function.
01/2008 to 01/2011Linköping University
  • Postdoctoral fellow in the Div.
  • Cell Biology Projects on brain circuits in drug addiction and inflammation.
  • Mouse genetic model with a floxed allele of mPGES-1gene & Generated a DNA targeting vector for a conditional mutation of mGluR5 gene & Generated one inducible and three constitutive (Bac) neuron and endothelial cell specific-Cre transgenic model & Contributed to the manuscript preparation.
01/2006 to 01/2008German Cancer Research Center DKFZ
  • Postdoctoral fellow in the Dept.
  • Molecular Biology of the Cell I / A02 Projects on neurological mechanisms of puberty onset.
  • Four Bac mouse transgenic lines & Conditional mouse models of Gpr54 and Gpr56 genes & Performed in vivo experiments with cell-specific knockout and knock-in animals & Constructed and tested for efficacy shRNA lentiviral vectors against Rb gene ( collaboration with Prof.
  • S.
  • Berger, DKFZ) & Generated a liver specific HCC mouse model ( collaboration with Prof.
  • Dr.
  • A.
  • Nordheim,Tuebingen University) & Generated osteoblast specific knock-out the glucocorticoid receptor (GR) (a joint effort with Dr.
  • Jan Tuckermann, Fritz Lipmann Institute) & Studied the role of Cox1 and Cox2 enzymes in the development of pulmonary arterial hypertension (PAH), participated in the manuscripts preparations ( collaboration with Prof.
  • Dr.
  • Tc.
  • Loukanov, Dep.
  • of Cardiac Surgery, University of Heidelberg).
Experimental chemist01/1998 to 01/2000EMAILCHIM GmbH, Bulgaria
  • Played an active role in collaborative projects with BASF Ludwigshafen, Germany.
Education
PhD Degree: Natural ScienceGPA: Magna cum LaudeNatural Science Magna cum Laude Heidelberg "A novel method to interfere with gene expression in mice using lentiviral transgenesis" Explored RNAi technology in vivo to knock-down a gene in mice Master in Molecular Biology and Biochemistry· Sofia University · Sofia "Generation of transgenic tobacco plants resistant to abiotic stress" Explored Agrobacterium tumefaciens to introduce useful genes in Tobacco plants High school: Technical School for Chemical Industry and Biotechnology · Sofia
Publications
Sexual differentiation of the brain isdetermined by perinatal kisspeptin-GnRH neuron signaling J. Clarkson, E. Busby, M. Kirilov, G. Schutz, N. M Sherwood and A. E Herbison J Neurosci. 2014 Nov 12;34(46):15297-305. doi: 10.1523/JNEUROSCI.3061-14.2014. Dysregulated SRF triggers formation of hepatocellular carcinoma S. Ohrnberger., A. Thavamani , A. Braeuning , D. Lipka , M. Kirilov , R. Geffers , S. Authenrieth , M . Bonin , M . Schwarz , G. Schütz , P. Schirmacher , C. Plass ,T. Longerich and A. Nordheim Hepatology. 2014 Sep 29. doi: 10.1002/hep.27539. microRNA-379 couples glucocorticoid hormones to metabolic dysfunction in obesity R. De Guia, A. Rose, A. Sommerfeld, O. Seibert, D. Strzoda, A. Zota, Y. Feuchter, A. Krones-Herzig, T. Sijmonsma, M. Kirilov, C. Sticht, N. Klöting, M . Blueher, M . Berriel Diaz, S. Herzig Submitted to the Journal of Clinical Investigation) Brain endothelial prostaglandin E2 synthesis mediates inflammatory fever Daniel Björk Wilhelms, Milen Kirilov , Elahe M irrasekhian, Anna Eskilsson, Unn Kugelberg Örtegren, Christine Kla, Dirk Ridder, Harvey R. Herschman, M arkus Schwaninger, Anders Blomqvist, David Engblom J Neurosci. 2014 Aug 27;34(35):11684-90. doi: 10.1523/JNEUROSCI.1838-14.2014 Reduced expression of the retinoblastoma protein shows that the related signaling pathway is essential for mediating the antineoplastic activity of erufosine M . Zaharieva, M. Kirilov, M . Chai, S. Berger, S. Konstantinov and M . Berger PLoS One. 2014 Jul 2;9(7):e100950. doi: 10.1371/journal.pone.0100950. eCollection Dependence of fertility on kisspeptin-Gpr54 signaling at the GnRH neuron. Kirilov M, Clarkson J, Liu X, Roa J, Campos P, Porteous R, Schütz G, Herbison A Nat Commun. 2013;4:2492. doi: 10.1038/ncomms3492 Cyclooxygenase-2 expression in lung in patients with congenital heart malformations and pulmonary arterial hypertension. Loukanov T, Jaschinski C, Kirilov M, Klimpel H, Karck M , Gorenflo M . Thorac Cardiovasc Surg. 2013 Jun;61(4):307-11 doi: 10.1055/s-0033-1337446. Epub 2013 M ay 16 Glucocorticoids suppress bone formation by attenuating osteoblast differentiation via the monomeric glucocorticoid receptor. Rauch A, Seitz S, Baschant U, Schilling AF, Illing A, Stride B, Kirilov M, M andic V, Takacz A, Schmidt-Ullrich R, Ostermay S, Schinke T, Spanbroek R, Zaiss M M , Angel PE, Lerner UH, David JP, Reichardt HM , Amling M , Schütz G, Tuckermann JP. Cell Metab. 2010 Jun 9;11(6):517-31. doi: 10.1016/j.cmet.2010.05.005. Localization of cyclo-oxygenase-2 in human recurrent colorectal cancer. Loukanov Ts, Kirilov M, Fürstenberger G, M üller-Decker K. Clin Invest Med. 2010 Feb 1;33(1):E22-9. Germ line transmission and expression of an RNAi cassette in mice generated by a lentiviral vector system. Kirilov M, Chai M , van der Hoeven F, Kloz U, Schmid W, Schütz G. Transgenic Res. 2007 Dec;16(6):783-93. Epub 2007 Aug 8.
Languages
English (fluent) , German (Goethe B1, ongoing language studies)
Skills
Photoshop, approach, Bulgarian, English, fat, German, Imaging, Languages, Microsoft Office, novel, Prism, Project planning, Research, Rip, Supervision
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Resume Overview

Companies Worked For:

  • German Cancer Research Center DKFZ
  • Linköping University
  • EMAILCHIM GmbH, Bulgaria

Job Titles Held:

  • Experimental chemist

Degrees

  • PhD Degree : Natural Science

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