Biologist resume example with 18+ years of experience

(555) 432-1000,
Montgomery Street, San Francisco, CA 94105

Research Biologist with 18 years in laboratory research. Accomplished in generating and applying new protocols and technologies.

  • Immunofluorescence assay expertise
  • Microscopy
  • DNA extraction
  • PCR
  • Mosquitoes dissections
  • Malarial cultures
  • Parasitic diseases
  • Drug susceptibility testings
Emory University Atlanta, GA Expected in May 1996 Bachelor of Science : Biology and Ecology - GPA :
University of Science , Expected in March 2010 Master of Arts : English Art & Technology - GPA :

Continuing Education in teaching

Graduated Summa Cum Lade

, Expected in Master of Education : - GPA : TESOL cum laude
Job Related Trainings Counterintelligence and Insider Threats Training Record Management Training Safety Survival Skills Part IV: Occupant Emergency Program STARLIMS & ELIMS Servers Training Bloodborne Pathogens CDC Hazard Communication Training Update Hazardous Chemical Waste Management for CDC Workers Radiation Safety Training Safety Survival Skills Part I: General Responsibility Security Awareness Training Online Waste Ticket System Defensive Driving Dual Use Research of Concern Safety Survival Skills Part II: Laboratory Safety The NoFear Act American Heart Association Heartsaver CPR/AED Training Walking and Working Surfaces Crime Prevention Safety Seminar Chemical Hazards Tracking Seminar Safety Office Test Hazardous Chemical Inventory Clinic Issues Safety Seminar Update on Hepatitis Viruses: Prevention and Control Biological Safety Cabinets and Ergonomic Excel Intermediate Science vs. Religion

Provided plasmodium sp. antigen for RDT test panel

Department Of Defense - Biologist
Niagara Falls, NY, 02/1997 - 07/2015
  • Research Biologist-32-40 hours per week Centers for Disease Control and Prevention Center for Global Health Division of Parasitic Diseases and Malaria Research, Techniques, Analysis and Data Management Responsible for performing various laboratory techniques, experiments and studies, maintenance, and evaluation parasitic microorganisms' susceptibility to drugs, while ensuring proper collection and processing of data Adapt new strains of malaria parasite, processing the specimen for in vitro culture, evaluating growth of parasites through daily parasitemia counts from blood smears, comparing daily results to determine adaptation of parasites to their new conditions Cultivate and maintain collected blood specimens from outside agencies and hospitals, after recording and entering specimen's information into laboratory data in accordance with quality standards and following records management requirements, identify origination of specimen in order to assign name and code to the specimen Contact the source where the samples were collected in cases where information may be missing or the standard of protocol were not followed by the senders Cooperate with other laboratories to ensure that incoming specimens are processed and triaged maintaining chain of custody, aliquoting specimens received, using aseptic techniques, to be processed and tested as needed, recording specimen information, and reporting results back to source as requested Perform recurring, well-precedented standard techniques to identify and characterize parasites when specimens are received from the field and/or hospital, blood typing the samples, preparing smears and staining them for identification and characterization through microscopy and/or DNA amplification and PCR Maintain blood stage cultures for gametocyte production and study, making adjustment in methods and procedures to ensure that the gametocytes formed will be infectious Identify any unusual or unexpected results and their probable causes in test results, before making adjustments to protocols and reevaluating methods and techniques Review printed articles and literatures to keep up-to-date with new applications and techniques, for example installing a more updated version of software to our computer allows for a more efficient way to analyze test results, with more features for graphing results Prepare drug concentrations for susceptibility testing of malaria parasites, adjusting concentrations according to sensitivity of strains and analysis of test results Evaluate tests results from drug susceptibility testing, comparing results with those obtained from previous experiments to determine whether data generated are accurate, valid, and consistent Conduct experiments using new drugs received, calculating and adjusting concentration of drugs and parasitemia to develop and validate the effectiveness of the new drugs.
  • Experiment with newly culture-adapted parasites to validate their sensitivity to current malarial treatments or novel drugs and assays Perform detection of malaria parasites by nested PCR amplification of dried blood spots on filter papers over 20 years old, testing for multi-species infection Collaborates with CaridianBCT Inc.
  • to inactivation Plasmodium falciparum in plasma and platelet concentrates with riboflavin and UV light Enter test data into computerize statistical format using XLFit program for analysis Prepare written summaries and report data analysis of experimental findings Scanned historical and current publications for webpage access Biotinlyation of monoclonal antibodies for malaria antigen detection and capture ELISA with anti-aldolase MAb Involve in a study using PET-PCR method for the molecular detection of malaria parasites from dried blood spots as part of a malaria bed net surveillance study in Haiti (2013) Assist with the malaria rapid diagnostic tests, as part of the WHO-FIND Malaria RDT project, preparing blood stage Plasmodium species schizonts as antigen Record-keeping, Reporting, and Organizing Skills Responsible for inventory of resources, and equipment need for studies and experiments Search and review catalogs for supplies needed for experiments Keep accurate records of routine procedures and experiments performed Gather information, prepare order forms, and submit requests for laboratory equipment and supplies Write protocols and update procedures for all essential documents needed Identify and experiment new technique reports by other scientific investigators, studies, and projects to find more efficient ways of testing and performing routine works, for example converting to using PET-PCR for quick results of samples being analyzed, versus the use of nested PCR when samples do not contain multiple malaria species Accomplish several projects with innovative approaches, rotating culture flasks to increase the rate of parasite growth and to avoid multiple infection of red blood cells, as a result cultures can be tested in a timely manner Daily and routinely record and maintain laboratory data of all activities in accordance to standard record-keeping requirements Organize research materials and information, supplies and equipment to carry-out experiments in an assigned work area, so as to be efficient with the work to be performed Prepare and present reports on a scientific investigation of how Plasmodium falciparum can be inactivated using riboflavin and UV light to supervisors, before it presenting it as a poster at conferences (see Presentations) before presenting the study at the XXXth International Congress: International Society of Blood Transfusion in Macao SAR, China.
  • Prepare analyses of drug susceptibility tests and reports results to supervisors and colleagues in a format that can be easily followed and understood, with data clearly presented, allowing for accurate interpretation of data and results Keep inventory of specimens, updating and entering new specimens added and removed from data Order and purchase laboratory equipment and supplies through CDC glassware sites Report results of daily blood smears of infected primates and in vitro cultures to supervisor Prioritize daily workloads to meet dead-lines Quality control and regulations, including calibrating and operating laboratory instruments used for drug susceptibility testing of parasites Perform preventive maintenance on equipment, pipets, and other instruments, making sure that they meet standard operating requirements and keeping record of such.
  • Calibrate and normalize TopCount instrument used for analyzing radioactive drug plates Meet all training and safety requirements for the use of radioactive materials such as 3H-hypoxanthine and disposal of it Troubleshooting instrument or problems when encountered during various projects, also contacting companies to assist in correcting the problems Responsible for quality control of laboratory eyewashes Assist, Train and Supervise Train visiting guest researchers and new employees on daily maintenance of parasitic cultures Gather slides and information needed to train and conduct graduate students and medical students on methodology to smear preparation and malarial microscopy Work with safety representative in assurance that group was in compliance with all biosafety regulations and procedures Assist new employees in meeting their safety and security requirements Collaborate with colleagues in conducting a detection limit test from the inactivation of Plasmodium falciparum Assist in RDT quality-control testing, preparing antigens purification and testing, dispensing additional samples into vials for ELISA, and make Earle Perez thick smears Review papers for publication in scientific journals and assist in preparation of data for poster presentations Technical Experience Dissection of mosquito guts and glands Skilled in malaria microscopy Diagnosis of human and primate malaria Trained in Biosafety Level 2 ELISA, IFA DNA extraction from body fluids, cells, and filter papers Polymerase Chain Reaction (PCR) and nested PCR Real-time PCR including PET-PCR Electrophoresis of PCR of amplified DNA Process new patient isolates Use of TopCount NXT and cell harvester Preparation of drug assay for susceptibility testing Malaria parasite culture Synchronize and purify blood stages of parasites by treatment with 5% Sorbitol and Percoll Counting parasite density with a hemocytometer Blood typing Use of a Coulter Counter Use and knowledge of radioactive materials Preparation of media and reagent for tissues and parasitic cultures Staining of tissue sections, particularly liver sections Work with aseptic technique and conditions Preparation of blood smears, both thick and thin Cryopreservation and thawing of parasites Giemas stain both thick and thin blood smears Formatting labels for specimens for cryo-storage Biotinylation of monoclonal antibodies.


Fluent in reading, writing and speaking English, Chinese, and Thai

Conversational Lao

Professional Affiliations
Keil Shawn D, Kiser Patti, Sullivan James J, Claire Jessica, Reddy Heather L, Avery Anne, Goodrich Raymond P. Inactivation of Plasmodium spp. in plasma and platelet concentrates using riboflavin and ultraviolet light. Transfusion. 2013 October; 53(10): 2278-2286. Collins William E, Sullivan JoAnn S, Hall Patrice, Ruebush Trenton K, Williams Allison, Grady Katharine K, Bounngaseng Amy, Nace Douglas, Williams Tyrone, Huber Curtis, Galland G Gale, Barnwell John W, Sullivan James J. Adaptation of a multi-drug resistant strain of Plasmodium falciparum from Peru to Aotus lemurinus griseimembra, A. nancymaae, and A. vociferans monkeys. The American Journal of Tropical Medicine and Hygiene 2007; 77(2): 261-5. Sullivan JoAnn S, Stewart Ann, Bounngaseng Amy, Sullivan James J, Nace Douglas, Williams Allison, Galland G Gale, Williams Tyrone, Henry Fleetwood, Collins William E. Observations on the exoerythrocytic stages of different isolates of Plasmodium cynomolgi in hepatocytes of New World aotus and Saimiri monkeys. The Journal of Parasitology 2006, 92(1): 202-5. Collins William E, Barnwell John W, Sullivan JoAnn S, Nace Doulgas, Williams Tyrone, Bounngaseng Amy, Robert Jacquelin, Strobert Elizabeth, McClure Harold, Saul Allan, Long Carole A. Assessment of transmission-blocking activity of candidate Pvs25 vaccine using gametocytes from chimpanzees. The American Journal of tropical medicine and hygiene. 2006; 74(2): 215-21. Sullivan JS, Bounngaseng A, Stewart A, Sullivan JJ, Galland GG, Fleetwood H, William EC. Infection of Saimiri boliviensis monkeys with Plasmodium coatneyi. Journal of Parasitology. 2005 April; 91(2): 479-81. Collins WE, Sullivan JS, Galland GG, Barnwell JW, Nace D, Williams A, Williams T, Bounngaseng A. Rio Meta strain of Plasmodium vivax in New World monkeys and anopheline mosquitoes. Journal of Parasitology. 2004 August; 90 (4): 685-8. Sullivan JS, Sullivan JJ, Williams A, Grady KK, Bounngaseng A, Huber CS, Nace D, Williams T, Galland GG, Barnwell JW, Collins WE. Adaptation of a strain of Plasmodium falciparum from Ghana to Aotus lemurinus griseimembra, A. nancymai, and A. vociferans monkeys. American Journal of Tropical Medicine and Hygiene. 2003 December; 69 (6): 593-600. Collins WE, Sullivan JS, Nace D, Williams T, Sullivan JJ, Galland GG, Grady KK, Bounngaseng A. Experimental infection of Anopheles farauti with different species of Plasmodium. Journal of Parasitology. 2002 April; 88(2): 295-8. Sullivan JS, Strobert E, Yang C, Morris CL, Galland GG, Richardson BB, Bounngaseng A, Kendall J, McClure H, Collins WE Adaptation of a strain of Plasmodium vivax from India to New World monkeys, chimpanzees, and anopheline mosquitoes. The Journal of Parasitology. 2001, 87(6): 1398-403. PRESENTATION & ABSTRACTS: J. Sullivan, AS Bounngaseng, HL Reddy, SD Keil, RP Goodrich. Pathogen Inactivation of Plasmodium Falciparum in Plasma and Platelet Concentrates with Riboflavin and UV Light. Poster presented at the XXXth International Congress: International Society of Blood Transfusion; June 7-12, 2008; Macao SAR, China. Collins WE, Sullivan JS, Nace D, Williams T, Sullivan JJ, Galland GG, Grady KK, Bounngaseng A. Experimental infection of Anopheles farauti with different species of Plasmodium. Poster presented at the American Society for Topical Medicine and Hygiene, December 3-7, 2003; Philadelphia, PA. Sullivan JS, Strobert E, Yang C, Morris CL, Galland GG, Richardson BB, Bounngaseng A, Kendall J, McClure H, Collins WE Adaptation of a strain of Plasmodium vivax from India to New World monkeys, chimpanzees, and anopheline mosquitoes. Poster presented at the American Society for Topical Medicine and Hygiene, 1997; Orlando, FL.
Additional Information
  • Interested in part-time and full-time

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School Attended

  • Emory University
  • University of Science

Job Titles Held:

  • Biologist


  • Bachelor of Science
  • Master of Arts
  • Master of Education

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