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Biological Scientist Resume Example

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BIOLOGICAL SCIENTIST
Professional Summary

Experienced public health laboratory scientist with three years of state public health laboratory experience focused in virology and close to three years as a biological scientist for a private company managing a government contract which provides testing for an environmental surveillance program.

Core Qualifications
  • Knowledge of basic laboratory safety procedures
  • Capable of performing aseptic, autoclave, and sterilization techniques
  • RNA/DNA extraction (manual kits, QiaCube, MagNA Pure)
  • Knowledge in performing real-time qRT-PCR (ABI 7500 Fast Dx and Roche LightCycler 96 well and LightCycler 2.0 capillary)
  • Reagent quality control & assurance
  • Instrument quality control & assurance
  • Experienced in media preparation for a variety of assays
  • BSL-3 laboratory trained
  • Ability to gather, interpret, and document experimental results
  • Knowledge of instructing individuals and groups
Experience
Biological Scientist
October 2012 to CurrentA-TEK, INC. － Jacksonville, FL

Responsibilities

  • Accessioning and processing environmental samples, as well as two quality assurance samples per day, while keeping chain of custody until samples are tested and results are confirmed
  • Performing DNA nucleic acid high throughput vacuum extraction using using stacked Millipore 96 well filter plates
  • Also performed Millipore single tube extraction using Amicon Ultra Centrifugal filters when extracting a smaller sample size
  • Screening purified DNA for select pathogenic organisms using real-time qRT-PCR specifically the Taqman assay
  • Analyzing and reporting daily results using Laboratory Response Network messenger website
  • Performing verification runs on positive quality assurance samples using appropriate primer and probe sets designed for verification of the particular agent the sample screened positive for
  • Performed monthly quality control testing on current reagents in use as well as when new reagent lots are received
  • Used MS Excel to conduct reagent control charting to monitor changes in daily used positive controls and investigated and troubleshooted control failures
  • Performed weekly environmental swabs on multiple surfaces throughout the laboratory for contamination surveillance in an effort to reduce cross-contamination
  • Assisted in scanning and record keeping of paperwork
  • Archiving samples and shipping them to NSWC according to timetables
  • Performed monthly inventory of stock and reagents
  • Performed all work with strict adherence to SOPs
Training Fellow
September 2011 to August 2012APHL/CDC Emerging Infectious Disease Fellowship － Albuquerque, NM

Responsibilites

  • Attempted to develop and validate a multiplex real-time qRT-PCR assay for the detection of WNV, WEEV, and SLEV
  • Performed in silico development of primer/probe sets for use in qRT-PCR detection of WNV, WEEV, and SLEV using BioEdit, PrimerExpress, and BLAST
  • Performed agarose gel electrophoresis in order to troubleshoot potential primer-dimers and hairpin loop issues with primer sets
  • Implemented and validated a method for arboviral RNA extraction from mosquito pools using the Tecan EVO 100 platform
  • Observed in rotation and was in instructed in various testing methods performed by different laboratory sections including: serology, food and general microbiology, and environmental water testing
  • Observed and was in instructed in various testing methods performed by the New Mexico Department of Agriculture Veterinary Diagnostic Services (VDS) including performing fusion protein ELISAs on horse serum to test for Equine infectious anemia virus and performing indirect immunofluorescence assay on canine serum to test for the presence of Ehrlichia canis, including the use of a light microscope to confirm positive or negative results
  • Observed and performed different techniques in trapping mosquitoes for arboviral surveillance
  • Trained in separating mosquitoes into pools by gender and species
Biological Scientist I
August 2009 to September 2011Florida Department of Health-Bureau of Public Health Laboratories － Albuquerque, NM

Responsibilites

  • Received and processed avian sera specimens for the Arbovirus Sentinel Chicken Surveillance program
  • Performed hemagglutination inhibition (HI) assay on specimens to screen for the presence of antibodies specific to alpha- or flaviviruses
  • Prepared reagents for and conducted in house IgM capture ELISAs on HI positive specimens to confirm presence of antibodies specific to WNV, SLEV, and/or EEEV
  • Also performed IgM capture and IgG sandwich ELISAs for clinical specimen testing. Clinical specimen ELISAs included testing for the same arboviruses as the avian sera specimens with the inclusion of DENV as well
  • Analyzed and provided results to the Virology supervisor for reporting and faxed final results to counties participating in the Arbovirus Sentinel Chicken Surveillance program
  • Performed necropsies on horse brain and avian specimens to recover tissue as well as processed mosquito pools for viral RNA extraction
  • Conducted single tube extraction of necropsied tissues/mosquito pools for purified viral RNA using QIAamp Viral RNA Mini Kit
  • On occasion automated extraction would be used on mosquito pools for viral RNA extraction using the MagnaPure LC
  • Prepared master mix for WNV, SLEV, EEEV, and/or DENV screen testing using real-time qRT-PCR on the ABI 7500
  • Performed direct immunofluorescence assay (DFA) on clinical specimens to detect the presence of herpes simplex virus 1 and 2 using a light microscope. Also performed DFAs for the detection of Influenza A and B as well as for parainfluenza
  • Participation in quality control and quality assurance to ensure compliance with regulatory policy
Education
Master of Public Health : Global communicable disease, 2011University of South Florida － Tampa, FL, United States
Graduate Certificate : Infection Control, 2011University of South Florida － Tampa, FL, United States
Bachelor of Science : Health Science, 2008Florida International University － Doral, FL, United States
Volunteer

Florida Department of Health-Bureau of Public Health Laboratories

Jacksonville, FL
Volunteer
2012-current
  • Volunteered in the Virology department performing viral DNA or RNA extractions of clinical samples for influenza A & B, respiratory panels, enterovirus, norovirus, dengue virus, and herpes simplex virus 1 & 2 using the MagnaPure LC
  • Ran real-time qRT-PCR on ABI 7500 fast Dx for most clinical samples, except for when testing for herpes simplex virus 1 & 2 and norovirus G1 and G2 in which the LightCycler 2.0 with capillaries were used
  • Hydrated and aliquoted primer and probe sets for various molecular assays
​​
​​New Mexico Department of Health-Scientific Laboratory Division
Albuquerque, NM
Translator
2011
  • Partnered with NMDOH-SLD's laboratory director, various department supervisors, and biosafety officer and assisted their participation with the World Health Organization's laboratory twinning initiative project
  • Performed as a translator and guide for the biosafety representatives of the Central Public Health Laboratory of Paraguay (the national laboratory) in order to provide them with a thorough understanding of how the framework of SLD's biosafety and biosecurity plans are implemented and how they function within the laboratory
  • The goal of the project was to provide information and mentor the representatives in order for them to eventually be able to develop and implement a similar plan for the Central Public Health Laboratory in Asunción, Paraguay.
Research

University of South Florida

Location: La Ceibita, Dominican Republic

Master's Special Project

Efficacy Testing Of Filterpure Ceramic Filters


  • Established communications with the Instituto Superior de Agricultura in the Dominican Republic to form a partnership that allowed use of the institute's laboratory facility for a water safety project
  • Setup the laboratory space given to the team by the institute by sterilizing supplies, preparing media, and setting up plates to prepare for assays to be performed the following day
  • Managed a team of students in aseptically collecting drinking water samples in an isolated region of the country
  • Performed membrane filtration technique on intervention and control group samples to gauge the efficacy of Filterpure ceramic filters in removing fecal coliforms, specifically E. coli, in drinking water to improve the quality according to WHO guidelines
  • Recorded and analyzed data for written master's special project report
Licenses
  • Florida Board of Clinical Laboratory Personnel -Licensed Public Health Laboratory Technician in Microbiology, 2011-current, License #: PHTC 43363
  • Certified in Shipping Biological Substances, Infectious Substances and Related Hazards 2015-2017
  • Select Agent Clearance-Tampa, FL, Florida Department of Health, Bureau of Public Health Laboratories, 2009-2010
  • Select Agent Clearance-Jacksonville, FL, Florida Department of Health, Bureau of Public Health Laboratories, 2012-current
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Resume Overview

Companies Worked For:

  • A-TEK, INC.
  • APHL/CDC Emerging Infectious Disease Fellowship
  • Florida Department of Health-Bureau of Public Health Laboratories

School Attended

  • University of South Florida
  • Florida International University

Job Titles Held:

  • Biological Scientist
  • Training Fellow
  • Biological Scientist I

Degrees

  • Master of Public Health : Global communicable disease , 2011
    Graduate Certificate : Infection Control , 2011
    Bachelor of Science : Health Science , 2008

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