Manager resume example with 17+ years of experience
JessicaClaire
Montgomery Street, San Francisco, CA94105609 Johnson Ave., 49204, Tulsa, OK
Home: (555) 432-1000
Cell:
resumesample@example.com
Summary
R&D and cGMP development Manager with a PhD in Chemistry, > 2 years' cGMP experience in API and generic drug manufacturing.
*Over 9 years' experience in Peptide and small molecules synthesis (Solid and solution phase), cleavage, purification, characterization, and assay development via a variety of spectroscopic technique.
*Outstanding record of innovation and success in multiple projects over the course of graduate, post-doctoral and industrial career with a proven publication record.
Accomplishments
Organic Synthesis and Purification: Peptide/peptidomimetics synthesis, peptide and protein conjugation PEGylation, manual and automated peptide synthesis, multi-gram to kilogram API scale up and production, peptide analytical method validation using RP-HPLC, silica column chromatography.
Analytical Instruments: NMR (1H, 13C, 113Cd, 199Hg, 207Pb NMR and Mass Spectrometry, HPLC, GC, LC/ESI-MS, MALDI-TOF, Circular dichroism (CD), FT-IR, Near-IR, UV-Vis, Fluorescence spectrophotometer, Electron Paramagnetic Resonance (EPR) spectroscopy, Cyclic voltammetry (CV), Detection and quantification of trace amount of PEG using CAD detector.
Skills
Guest services
Inventory control procedures
Merchandising expertise
Loss prevention
Cash register operations
Product promotions
Experience
Manager, 08/2017 to Present Jones Lange Lasalle Inc. – Fresno, TX,
Published 12 peer reviewed research papers, attained several national and international conferences for poster and seminar presentations.
Manages, prioritize and directs the research and development programs to meet organizational needs.
Analyze scientific data, prepare project status reports and present as needed.
Oversees complex research projects, analyzes results and provides recommendations based on findings.
Develop documentation for new product projects.
Collaborate with cross functional department/team in QC, QA, Operations, Production and Finance.
Comply with cGMP requirements and assist the R&D team in development of API products.
Manages subordinate staff in the day-to-day performance of their jobs.
Sr. Chemist, 06/2015 to 07/2017 Ecolab Inc. – Beckley, WV,
Led several business strategic projects from synthesis, purification, lyophilization to final reconstitution as final API products by following all regulatory guidelines, cGMP guidelines and Environmental Health and Safety regulations.
Provided insights into production optimization; wrote investigation, technical protocol and reports, deviation, change requirements.
Developed purification schemes to purify peptide in large scale.
Trained entry level chemists in upstream and downstream process including use of analytical HPLC and chromeleon software.
Trained on Oligonucleotide upstream and downstream manufacturing process.
Research Associate Scientist, 09/2014 to 05/2015 Rutgers University – New Brunswick, NJ,
Studied transport mechanism of environmental pollutant (mercury) into soil bacteria.
Studied how mercury gets converted into methyl-mercury inside the bacterial cells.
Postdoctoral Research Associate, 07/2011 to 08/2014 Harris County – Houston, TX,
Developed artificial, biologically-inspired metallopeptides that have great potential in the area of chemical catalysis, tremendous importance to drug discovery, and industrial catalysis.
Synthesized/purified 3-amino acid cyclic and linear peptides including unnatural amino acids.
Trained graduate and undergraduate students on designing metal binding peptides and use of spectroscopic technique such as UV-vis, NMR, CD, EPR, HPLC, ESI-MS etc.
Involved in manuscripts, NSF- grants writing; presented research findings in regional and national ACS conferences.
Taught Bio-inorganic chemistry course to graduate and undergraduate students as guest lecturer.
Postdoctoral Fellow, 06/2009 to 06/2011 Metropolitan State University Of Denver – Denver, CO,
Explored the nature of cadmium and mercury binding to proteins which helps elucidate the molecular mechanism of heavy metal toxicity in humans.
Developed lead-NMR (207Pb NMR) spectroscopy, a technique that had never been successful with thiol (sulfur) rich coordination environments of the very toxic lead ion (Pb2+) due to its difficulty in acquiring the Pb-NMR spectra.
Not only pioneered the use of 207Pb-NMR spectroscopy in thiol rich proteins, also designed protein molecules that bind selectively Pb2+-ion which can be detected by 207Pb-NMR.
Studied how Pb2+ ion coordinates with very important biological molecules glutathione (found in blood) and zinc finger proteins.
Synthesized 22 to 37 amino acids long peptides using a peptide synthesizer (ABI-433A).
Involved in manuscripts, NIH grant/proposal writing.
Graduate Teaching/Research Assistant, 08/2005 to 05/2009 University Of Nevada – City, STATE,
Gained bioorganic/bioinorganic synthetic skills, learned air-sensitive techniques and performed a number of biological assays and catalytic/mechanistic studies.
Performed a large number of research projects.
These involved the preparation and study of nickel containing metallopeptides as mimics for the cysteinate ligated metalloenzyme nickel containing superoxide dismutase (NiSOD).
Proved vital in understanding not only how NiSOD operates, but were also important in establishing how nickel-thiolates can tolerate O2.
Demonstrated why small nickel-thiolates could withstand oxidatively harsh conditions presented at the NiSOD active site.
Involved in manuscript writing; presented research findings in regional and national ACS conferences.
Instructed chemistry labs and quiz classes for 3 years (2005 - 2008).
Graduate Teaching Assistant, 08/2003 to 05/2005 Wayne State University – City, STATE,
Performed expression of the recombinant fusion protein (GST-MT) in E.
coli.
Isolated and purified expressed metallothionein protein using affinity chromatography, characterized the protein by SDS-PAGE and MALDI-TOF.
Purified thrombin digested metallothioneine (MT) protein from parent GST-MT protein.
Studied binding assay of MT to CdSe nanoparticles.
Taught general chemistry laboratory and quiz classes as a teaching assistant.
PROFESSIONAL SERVICES.
Education and Training
Ph.D: Bio-Inorganic Chemistry, Expected in May 2009 University of Nevada - Reno, NV GPA: Bio-Inorganic Chemistry Advisor: Prof. Jason Shearer), Dissertation: "Nickel Superoxide Dismutase: Insights into the Metalloenzyme Gained from Functional Metallopeptide Models".
M.S: Bio-Inorganic Chemistry, Expected in May, 2005 Wayne State University - Detroit, MI GPA: Bio-Inorganic Chemistry Advisor: Prof. David E. Benson), Thesis: "Developing Metallothionein Capped Cadmium Selenide (CdSe) Nanoparticles".
M.Sc: Inorganic Chemistry, Expected in 2000 Tribhuvan University - , GPA: Inorganic Chemistry
B.Sc: Chemistry & Biology, Expected in 1997 Tribhuvan University - Palpa, GPA: Chemistry & Biology
Publications
Protein expression, purification, characterization using SDS-PAGE, DNA extraction, PCR (limited)
* RESEARCH PUBLICATIONS (12 publications, > 320 citations)
*Shearer, J.*; Peck, K. L.; Schmitt, J. C. and Neupane, K. P. "Cysteinate Protonation at the ActiveSite of Nickel Superoxide Dismutase: Implication for the Mechanism of Superoxide Reduction" J. Am. Chem. Soc., 2014, 136, 16009-16022.
*Eshelman, M. R.; Aldous, A. R.; Neupane, K. P.; Kritzer, J. A.*, "Solution Structure of a designed cyclic peptide ligand for nickel and copper ions." Tetrahedron, 2014, 70, 7651-7654.
*Neupane, K. P.; Aldous, A. R.; Kritzer, J. A.*, "Metal-binding and redox properties of substituted lnear and cyclic ATCUN motifs", J. Inorg. Biochem., 2014, 139, 65-76.
*Neupane, K. P.; Aldous, A. R.; Kritzer, J. A.* "Macrocyclization of the ATCUN motif controls metal binding and catalysis" Inorg. Chem., 2013, 52, 2729-2735.
*Zampella, G.*; Neupane, K. P.; DiGioia, L. and Pecoraro, V. L.*, "The importance of stereochemically active lone pairs for influencing Pb(II) and As(III) protein binding", Chem. Eur. J., 2012, 18, 2040-2050.
*Neupane, K. P. and Pecoraro, V. L*. "Pb-207 NMR spectroscopy reveals that Pb(II) coordinates with glutathione (GSH) and tris-cysteine zinc finger protein (Cys2HisCys) in a PbS3 coordination environment" J. Inorg. Biochem. 2011, 105, 1030-1034.
*Neupane, K. P and Pecoraro, V. L.* "Probing a homoleptic PbS3 coordination environment in a designed peptide using Pb-207 NMR: Implications for understanding the molecular basis of lead toxicity" Angew. Chem. Int. Ed. 2010, 49, 8177-8180. (Rated as Very Important Paper (VIP) status)
*Shearer, J. *; Neupane, K. P.; Callan, P.E. "Metallopeptide based mimics with substituted histidines approximate a key Hydrogen bonding network in the metalloenzyme nickel superoxide dismutase" Inorg. Chem. 2009, 48, 10560-10571.
*Dupont, C., Neupane, K. P., Shearer, J.; Palenik, B.* "Diversity, function, and evolution of genes coding for putative Ni-containing superoxide dismutases" Environ. Microbiol., 2008, 10, 1831-1843.
*Neupane, K. P.; Gearty, K.; Francis, A.; Shearer, J.* "Probing variable axial ligation in nickel superoxide dismutase utilizing metallopeptide-based models: insight into the superoxide disproportionation mechanism" J. Am. Chem. Soc. 2007, 129, 14605-14618.
*Neupane, K. P.; Shearer J.* "The influence of amine/amide versus bisamide coordination in nickel superoxide dismutase" Inorg Chem. 2006, 45, 10552-10566.
*Aryal B. P.; Neupane K. P.; Sandros M. G.; Benson D. E.* "Metallothioneins initiate Semiconducting nanoparticle cellular toxicity" Small 2006, 1159-1163.
* SELECTED PRESENTATIONS
Oral:
*Neupane, K. P.; Aldous, A. R.; Kritzer, J. A. "Cu(II) and Ni(II) binding by ATCUN-like cyclic peptides", American Chemical Society, 38th Northeast Regional Meeting, Rochester, NY, Sep 30th-Oct 3rd, 2012.
*Neupane, K. P.; Pecoraro, V. L., "De novo Designed of Heavy metal (PbII & CdII) binding Peptides: Implications for Understanding the Basis of heavy metal Toxicity", International Conference on Advanced Materials and Nanotechnology, Kathmandu, Nepal, Oct 21st-Oct 23rd, 2011.
Poster:
*Neupane, K. P.; Aldous, A. R.; Kritzer, J. A, "Macrocyclization of the ATCUN motif controls metal binding and catalysis "247th ACS National Spring Meeting, Dallas, TX, March, 16-20, 2014.
*Neupane, K. P.; Aldous, A. R.; Kritzer, J. A, "Macrocyclization of the ATCUN motif controls metal binding and catalysis "245th ACS National Spring Meeting, New Orleans, LA, April, 07-11, 2013.
*Neupane, K. P.; Zastrow, M. and Pecoraro, V. L. "A thermodynamic study of Pb(II) binding to de novo designed peptides: Implications for Understanding the Molecular Basis of Lead Toxicity" Vaughan Symposium, University of Michigan, August, 2010.
*Neupane, K. P.; Zastrow, M. and Pecoraro, V. L. "A thermodynamic study of Pb(II) binding to de novo designed peptides and insight into Pb(II) toxicity", Biophysical Symposium, University of Michigan, April, 2010.
*Neupane, K. P.; Chakraborty, S.; Touw, D. S.; and Pecoraro, V. L. "Preference of Pb(II) Binding in Designed CoilSer Peptide", Vaughan Symposium, University of Michigan, August, 2009.
*Neupane, K. P., Shearer, J. "Nickel Superoxide Dismutase: Insight Gained from Peptide Based Models" Poster at 237th ACS National Meeting, Salt Lake City, UT, March, 2009.
*Neupane, K. P., Shearer, J. "A Peptide Based Nickel Superoxide Dismutase (NiSOD) Model Compound." Poster at 61st ACS Northwest Regional Meeting, Reno, NV, June, 2006.
*Neupane, K.P., Benson, D.E. "Developing Metallothionein Capped Cadmium Selenide Nanoparticles" Poster at 61st ACS Northwest Regional Meeting, Reno, NV, June, 2006.
AWARDS
*Travel Award 2009: By Graduate Student Association, University of Nevada, Reno (for 237th ACS National Meeting, Salt Lake City, UT; March 22-27, 2009
*National Institute of Health (NIH)-funded Postdoctoral Research Fellow (2009-2011, 2011-2014)
* AFFILIATION
*2008- date: ACS member
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