Protein expression, purification, characterization using SDS-PAGE, DNA extraction, PCR (limited)
* RESEARCH PUBLICATIONS (12 publications, > 320 citations)
*Shearer, J.*; Peck, K. L.; Schmitt, J. C. and Neupane, K. P. "Cysteinate Protonation at the ActiveSite of Nickel Superoxide Dismutase: Implication for the Mechanism of Superoxide Reduction" J. Am. Chem. Soc., 2014, 136, 16009-16022.
*Eshelman, M. R.; Aldous, A. R.; Neupane, K. P.; Kritzer, J. A.*, "Solution Structure of a designed cyclic peptide ligand for nickel and copper ions." Tetrahedron, 2014, 70, 7651-7654.
*Neupane, K. P.; Aldous, A. R.; Kritzer, J. A.*, "Metal-binding and redox properties of substituted lnear and cyclic ATCUN motifs", J. Inorg. Biochem., 2014, 139, 65-76.
*Neupane, K. P.; Aldous, A. R.; Kritzer, J. A.* "Macrocyclization of the ATCUN motif controls metal binding and catalysis" Inorg. Chem., 2013, 52, 2729-2735.
*Zampella, G.*; Neupane, K. P.; DiGioia, L. and Pecoraro, V. L.*, "The importance of stereochemically active lone pairs for influencing Pb(II) and As(III) protein binding", Chem. Eur. J., 2012, 18, 2040-2050.
*Neupane, K. P. and Pecoraro, V. L*. "Pb-207 NMR spectroscopy reveals that Pb(II) coordinates with glutathione (GSH) and tris-cysteine zinc finger protein (Cys2HisCys) in a PbS3 coordination environment" J. Inorg. Biochem. 2011, 105, 1030-1034.
*Neupane, K. P and Pecoraro, V. L.* "Probing a homoleptic PbS3 coordination environment in a designed peptide using Pb-207 NMR: Implications for understanding the molecular basis of lead toxicity" Angew. Chem. Int. Ed. 2010, 49, 8177-8180. (Rated as Very Important Paper (VIP) status)
*Shearer, J. *; Neupane, K. P.; Callan, P.E. "Metallopeptide based mimics with substituted histidines approximate a key Hydrogen bonding network in the metalloenzyme nickel superoxide dismutase" Inorg. Chem. 2009, 48, 10560-10571.
*Dupont, C., Neupane, K. P., Shearer, J.; Palenik, B.* "Diversity, function, and evolution of genes coding for putative Ni-containing superoxide dismutases" Environ. Microbiol., 2008, 10, 1831-1843.
*Neupane, K. P.; Gearty, K.; Francis, A.; Shearer, J.* "Probing variable axial ligation in nickel superoxide dismutase utilizing metallopeptide-based models: insight into the superoxide disproportionation mechanism" J. Am. Chem. Soc. 2007, 129, 14605-14618.
*Neupane, K. P.; Shearer J.* "The influence of amine/amide versus bisamide coordination in nickel superoxide dismutase" Inorg Chem. 2006, 45, 10552-10566.
*Aryal B. P.; Neupane K. P.; Sandros M. G.; Benson D. E.* "Metallothioneins initiate Semiconducting nanoparticle cellular toxicity" Small 2006, 1159-1163.
* SELECTED PRESENTATIONS
Oral:
*Neupane, K. P.; Aldous, A. R.; Kritzer, J. A. "Cu(II) and Ni(II) binding by ATCUN-like cyclic peptides", American Chemical Society, 38th Northeast Regional Meeting, Rochester, NY, Sep 30th-Oct 3rd, 2012.
*Neupane, K. P.; Pecoraro, V. L., "De novo Designed of Heavy metal (PbII & CdII) binding Peptides: Implications for Understanding the Basis of heavy metal Toxicity", International Conference on Advanced Materials and Nanotechnology, Kathmandu, Nepal, Oct 21st-Oct 23rd, 2011.
Poster:
*Neupane, K. P.; Aldous, A. R.; Kritzer, J. A, "Macrocyclization of the ATCUN motif controls metal binding and catalysis "247th ACS National Spring Meeting, Dallas, TX, March, 16-20, 2014.
*Neupane, K. P.; Aldous, A. R.; Kritzer, J. A, "Macrocyclization of the ATCUN motif controls metal binding and catalysis "245th ACS National Spring Meeting, New Orleans, LA, April, 07-11, 2013.
*Neupane, K. P.; Zastrow, M. and Pecoraro, V. L. "A thermodynamic study of Pb(II) binding to de novo designed peptides: Implications for Understanding the Molecular Basis of Lead Toxicity" Vaughan Symposium, University of Michigan, August, 2010.
*Neupane, K. P.; Zastrow, M. and Pecoraro, V. L. "A thermodynamic study of Pb(II) binding to de novo designed peptides and insight into Pb(II) toxicity", Biophysical Symposium, University of Michigan, April, 2010.
*Neupane, K. P.; Chakraborty, S.; Touw, D. S.; and Pecoraro, V. L. "Preference of Pb(II) Binding in Designed CoilSer Peptide", Vaughan Symposium, University of Michigan, August, 2009.
*Neupane, K. P., Shearer, J. "Nickel Superoxide Dismutase: Insight Gained from Peptide Based Models" Poster at 237th ACS National Meeting, Salt Lake City, UT, March, 2009.
*Neupane, K. P., Shearer, J. "A Peptide Based Nickel Superoxide Dismutase (NiSOD) Model Compound." Poster at 61st ACS Northwest Regional Meeting, Reno, NV, June, 2006.
*Neupane, K.P., Benson, D.E. "Developing Metallothionein Capped Cadmium Selenide Nanoparticles" Poster at 61st ACS Northwest Regional Meeting, Reno, NV, June, 2006.