Interdisciplinary and innovative scientist who has collaborated and led teams of scientists and nonscientists on various translational and basic science projects. Over 12 years of successful research experience in immunology, oncology, drug discovery, diabetes, obesity, cell biology and biochemistry as demonstrated by 7 publications in prestigious journals including Journal of Medicinal Chemistry, Cell and Cell Metabolism. History of communicating goals to multidisciplinary teams, playing a significant role in strengthening and expanding research relationships to establish strategic collaborations.
Protein Science: Recombinant protein engineering and purification, proteomics and enzyme kinetics
Molecular biology: Cell culture, microscopy, Gene cloning and expression, qRT-PCR gene expression analysis, designing and generation of recombinant retrovirus, adenovirus and lentivirus
Cancer drug Discovery: HTS assay, new drug screening, small molecule secondary metabolite purification, HPLC, NMR, mass spectrometry, cancer cell migration and metastasis assay
Oncology: Working experience with Cross functional teams, Establishing in vivo animal models of cancer, metabolism, target identification and metabolic pathway analysis
Rockefeller UniversityNew York, USA, NYPostdoc Associate
Research Trainings: Protein science, Cell culture, molecular biology and new target identification, drug discovery Broad research topic: To discover the novel therapeutic target of a potential anti-obesity drug Accomplishments: Established biochemistry and protein chemistry lab for a startup laboratory Developed animal models of obesity Established the protocol for primary adipocyte cell culture and gene expression analysis Testing the potential small molecule drug for anti obesity and anti diabetic activity Developing retroviral and adenoviral mammalian protein expression systems Trouble shooter in protein chemistry, immunology and biochemistry Top skills: In vivo drug delivery in mouse models, weight curve analysis, tolerance tests (GTT/ITT), ELISA, gene expression analysis (qPCR), immunoprecipitation and western blot, cell culture, stable cell line generation, preparation and propagation of recombinant retrovirus and adenovirus for protein purification.
Yale UniversityNew Haven, USA, CTPostgrad and Postdoc Associate
Research Trainings: Cancer Biology, Cell & Molecular Biology, Metabolomics, and Diabetes Broad research topic: Role of posttranslational protein modifications in regulation of metabolic pathways of animal models of cancer and diabetes Accomplishments: As a part of team I published five high impact original research publication such as Cell, Cell Metabolism and JBC and two review articles My work led to one small grant (one year funding) and one large grant (three year funding) to the laboratory Helped four PhD students and 4 undergraduate students and one high school student in designing and executing and troubleshooting in their laboratory activities Introduced new technologies (Bio-ID in laboratory for efficient and specific protein purification Actively participated as team in writing various laboratory protocols for IRB approval Executed proteomics, Protein complex purification and characterization of binding partners Conducted in-vivo tumor generation experiments Executed expression and purification of mammalian epitope tagged recombinant fat metabolism and glycolytic pathway enzymes for mass spectrometry Performed tissue immunohistochemistry and biochemical studies on human cancer patient samples Hands on experience of multiplex Flow cytometery and microscopy Top skills: Mammalian cell culture, gene expression analysis, recombinant protein expression and purification, proteomics, protein chemistry, in-vivo tumor generation, ELISA, gene expression analysis (qPCR), protein extraction, immunoprecipitation, immunohistochemistry, microscopy and western blot.
National Chemical Laboratory NCLPune, IndiaSenior Research Fellow
Research Trainings: Biochemistry, Fermentation, Cancer, Drug discovery, and Medicinal Chemistry.
Broad research topic: Identification and characterization of novel low molecular mass protease inhibitor anticancer drug candidates from microbial sources.
I purified and characterized two small molecule secondary metabolite natural product purification from bacterial sources Anticancer drug discovery work was published in very prestigious Journal of medicinal chemistry During my four year stay, I helped my PI in writing two prestigious grants that were funded Handling and maintenance C4 and C18 reverse phase HPLC High throughput (HTS) assay protocol development to screen anticancer drug candidates Carried out native protein purification for enzyme kinetics Sample preparation for NMR spectrometry and structure elucidation of small molecules and peptides Mass spectrometry (Q-TOF, MALDI-TOF) of small molecule drug candidates Cancer cell culture, metastatic and cell migration assays Testing novel drug candidates for anti-metastatic potential on various cancer cell lines Top skills: Small molecule purification, NMR, Mass spectrometry, HPLC, Cell culture, fermentation and enzyme kinetics National Centre for Cell Science (NCCS), Pune, India September, 2002- October 2005 Junior Research Fellow Research Trainings: Immunology, molecular Biology and Stem Cell Biology Broad research topic: Role of co-stimulatory signals in generation and maintenance of T-cell immunity Accomplishments I established the two projects which generated external funding for my PIs the laboratory Cellular immunology aseptic isolation of immune cells such as B-Cells, T-cells, macrophage and dendritic cells cytotoxic T-lymphocyte assay, and immunologic assays Adaptive transfer and lineage tracing experiments using flow cytometry (FACS) Immunogenic formulations, immunization, and production monoclonal and polyclonal antibodies rDNA technology, gene editing, engineering of plasmids and adenovirus, retrovirus and lentivirus DNA and RNA isolation, PCR, RT-qPCR, and siRNA/miRNA/shRNA mediated gene knockout techniques Embryonic stem cells culture and differentiation to other cell types.
Pune UniversityIndiaPh.D: BiotechnologyBiotechnology
G.N.D. UniversityAmritsar, IndiaM. S. (Masters): BiotechnologyBiotechnology
Immunology, Biochemistry, Fermentation &Microbial Technology,
Genetics, Genetic Engineering, and Biostatistics
University of AllahabadIndiaB.S: Chemistry and BiologyChemistry and Biology
Fundamental Chemistry, Analytical Chemistry, Organic Chemistry, Biochemistry Medicinal Chemistry, Genetics, Botany, Zoology, Anatomy, Physiology & Metabolism ACADEMIC EXCELANCE, HONORS AND SCHOLARSHIPS National Scholarship funded by Council of Scientific and Industrial Research (CSIR), Department of Human Resource Development, Govt. of India towards studies for PhD degree (2003-2008) Junior Research Fellow, funded by department of biotechnology, Govt. of India (2002-2003) Awardee of eligibility for Lectureship' awarded by University Grants Commission Govt. of India (2002) Cleared Graduate Aptitude Test In Engineering (GATE) conducted by Indian Institute of technology (IIT) Govt. of India (2002) National Merit Scholarship funded by department of biotechnology, Govt. of India Towards studies for
Ruan HB, Dietrich MO, Liu ZW, Zimmer MR, Li MD, Singh JP, Zhang K, Yin R, Wu J, Horvath TL, et al. O-GlcNAc transferase enables AgRP neurons to suppress browning of white fat. Cell. 2014;159(2):306-17. 2. Li MD, Ruan HB, Hughes ME, Lee JS, Singh JP, Jones SP, Nitabach MN, and Yang X. O-GlcNAc signaling entrains the circadian clock by inhibiting BMAL1/CLOCK ubiquitination. Cell metabolism. 2013;17(2):303-10. 3. Ruan HB, Singh JP, Li MD, Wu J, and Yang X. Cracking the O-GlcNAc code in metabolism. Trends in endocrinology and metabolism: TEM. 2013;24(6):301-9. 4. Li MD, Ruan HB, Singh JP, Zhao L, Zhao T, Azarhoush S, Wu J, Evans RM, and Yang X. O-GlcNAc transferase is involved in glucocorticoid receptor-mediated transrepression. The Journal of biological chemistry. 2012;287(16):12904-12. 5. Ruan HB, Han X, Li MD, Singh JP, Qian K, Azarhoush S, Zhao L, Bennett AM, Samuel VT, Wu J, et al. O-GlcNAc transferase/host cell factor C1 complex regulates gluconeogenesis by modulating PGC-1alpha stability. Cell metabolism. 2012;16(2):226-37. 6. Singh JP, Tamang S, Rajamohanan PR, Jima NC, Chakraborty G, Kundu GC, Gaikwad SM, and Khan MI. Isolation, structure, and functional elucidation of a modified pentapeptide, cysteine protease inhibitor (CPI-2081) from Streptomyces species 2081 that exhibit inhibitory effect on cancer cell migration. Journal of medicinal chemistry. 2010;53(14):5121-8. 7. Singh JP, Zhang K, Wu J, and Yang X. O-GlcNAc signaling in cancer metabolism and epigenetics. Cancer letters. 2015;356(2 Pt A):244-50. 8. Singh JP, Lee JS, Han X, Ruan HB, Li MD, Zhang K, Qian K, P.Lee, Staron M, Zhao T, et al. Bifunctional O-GlcNAcase targets pyruvate kinase M2 to regulate tumor growth. Manuscript ready. 2016.
Ph.D : Biotechnology Medicinal Chemistry, Biophysical Chemistry, Biochemistry, Molecular Biology,
Fermentation, Microbial Technology, and Crystallography M. S. (Masters) : Biotechnology Immunology, Biochemistry, Fermentation &Microbial Technology,
Genetics, Genetic Engineering, and Biostatistics B.S : Chemistry and Biology Fundamental Chemistry, Analytical Chemistry, Organic Chemistry, Biochemistry Medicinal Chemistry, Genetics, Botany, Zoology, Anatomy, Physiology & Metabolism ACADEMIC EXCELANCE, HONORS AND SCHOLARSHIPS National Scholarship funded by Council of Scientific and Industrial Research (CSIR), Department of Human Resource Development, Govt. of India towards studies for PhD degree (2003-2008) Junior Research Fellow, funded by department of biotechnology, Govt. of India (2002-2003) Awardee of eligibility for Lectureship' awarded by University Grants Commission Govt. of India (2002) Cleared Graduate Aptitude Test In Engineering (GATE) conducted by Indian Institute of technology (IIT) Govt. of India (2002) National Merit Scholarship funded by department of biotechnology, Govt. of India Towards studies for M.S. Degree
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