Staff Scientist, T Cell Immunology Resume Example
Skilled and accomplished cell biologist with 10+ years of experience in academic and industry settings. Strong background in mammalian cell culture and cell-based assay development. Enthusiastic researcher comfortable with working in cross-functional teams, learning cutting-edge technologies and developing novel applications. Eager to join new organization working in a fast-paced, stimulating R&D environment.
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- Design, conduct and validate single-cell immunoassays to characterize CAR-T cell populations on microfluidic devices (cytokine release, cytotoxicity, T cell activation and proliferation, metabolic and transcriptomic profiling).
- Train research associates and members of project teams on instruments and lab methodologies and manage day-to-day experiment execution.
- Actively interact with Operations, Software and Reagent teams to ensure effective technology transfer.
- Support Marketing team by generating data for customer-facing marketing collateral.
- Write content for user manuals/protocols, providing clear and comprehensive instructions for proper execution of processes on company's systems.
- Provide design suggestions and bug reports to software team during testing of development builds of company's proprietary software, resulting in release of production version of system for the analysis of assay data.
- Participate on project teams as biology technical lead to facilitate the team's activities and achievement of goals.
- Use of immunological techniques such as isolation of primary immune cell subsets, primary cell culture and expansion, transfection and viral transduction of immune cells, multicolor flow cytometry and cell-based functional assays.
- Contribute to the technical development of a synthetic system for selection and expansion of antigen-specific T cells.
- Serve as primary technical expert to establish novel automated, single-cell immunoassays for functional profiling of immune cells on microfluidic devices.
- Support technical development of a workflow for the identification of therapeutically relevant T cell receptors (TCRs).
- Fulfill project management responsibilities such as coordination of functional teams, budget estimation, resource allocation and scheduling for the release of new T cell applications on company's platforms.
- Provide accurate reporting on timelines, deliverables and project status to the executive team.
- Generate gene-edited primary human T cell lines using CRISPR-CAS9 technology.
- Develop assays for automated single-cell analysis of gene-edited T cells on microfluidic devices.
- Develop molecular biology pipelines for high-throughput validation of CRISPR-edited cell lines using single-cell sequencing.
- Manage collaborations with academic institutions, present research findings to senior staff and contribute to preparation of research reports, manuscripts and patent filings.
- Identify new targets of cancer therapeutics by high-throughput, automated siRNA screens.
- Establish a new line of research in the lab using human and mouse embryonic stem cells as model system.
- Dissect the function of deubiquitinating enzymes in human cancer cells and in human embryonic stem cell differentiation.
- As lab viral facility coordinator, collaborate with coordinators from different labs to establish the SOPs of the facility and to purchase and maintain equipment; train members of the lab on proper handling of lentivirus.
- As lab safety coordinator, accumulate extensive knowledge in hazardous materials handling; train new lab members; maintain detailed records of the chemical inventory and of the safety procedures of the lab.
- As lab microscopy facility coordinator, purchase and maintain epifluorescence and confocal microscopes; test demo version of microscopes and imaging softwares; train members of the lab on different microscopy and image analysis techniques.
- Oh E, Mark KG, Mocciaro A, Watson ER, Prabu JR, Cha DD, Kampmann M, Gamarra N, Zhou CY, Rape M. " Gene expression and cell identity controlled by anaphase-promoting complex". Nature. 2020 Mar;579(7797):136-140.
- Mocciaro A, Roth TL, Bennett HM, Soumillon M, Shah A, Hiatt J, Chapman K, Marson A, Lavieu G. "Light-activated cell identification and sorting (LACIS) for selection of edited clones on a nanofluidic device". Commun Biol. 2018 May 3;1:41.
- A.Mocciaro and M. Rape. “Emerging regulatory mechanisms in ubiquitin-dependent cell cycle control”. J Cell Sci. 2012 Jan 15;125(Pt 2):255-63.
- A.Mocciaro and E. Schiebel. “Cdc14: a highly conserved family of phosphatases with non-conserved functions?”. J Cell Sci. 2010 Sep 1;123(Pt 17):2867-76.
- A. Mocciaro, E. Berdougo, K. Zeng, E.Black, P. Vagnarelli,W. Earnshaw, D. Gillespie, P. Jallepalli and E. Schiebel.” Vertebrate cells genetically deficient for Cdc14A or Cdc14B retain DNA damage checkpoint proficiency but are impaired in DNA repair”. J Cell Biol. 2010 May 17;189(4):631-9.
Methods for assaying biological cells in a microfluidic device
Patent date: Filed Nov 1, 2019
Patent issuer and number: WO2020092975A2
Proto-Antigen-Presenting Synthetic Surfaces, Activated T Cells, and Uses Thereof
Patent date: Filed Oct 17, 2019
Patent issuer and number: US20200139362A1
Methods for Selection and Generation of Genome Edited T Cells
Patent date: Filed Jun 27, 2019
Patent issuer and number: US20200048606A1
Methods, Systems and Devices for Selection and Generation of Genome Edited Clones
Patent date: Issued Mar 26, 2019
Patent issuer and number: US10239058B2
General functional assay
Patent date: Filed Dec 28, 2018
Patent issuer and number: WO2019133874A1
Antigen-presenting synthetic surfaces, covalently functionalized surfaces, activated T cells, and uses thereof
Patent date: Filed Jul 20, 2018
Patent issuer and number: WO2019018801A1
Tumor Infiltrating Cells Engineered to Express a Pro-Inflammatory Polypeptide
Patent date: Filed Apr 14, 2017
Patent issuer and number: US20170224734A1
November 2019
Poster presentation at SITC, National Harbor, MD.
"Development of an antigen-presenting bead kit for activation and expansion of human antigen-specific T cells"
March 2017
Poster presentation at AGBT, Hollywood, FL.
"Massively Parallel Phenotyping, Culturing, Assaying and Sequencing of Single Cells on an OptoFluidic™ Chip"
September 2015
Oral presentation at the CIRM Stem Cell Fellows Symposium, Berkeley, CA.
“Transcriptional regulation of human embryonic stem cell differentiation by the deubiquitinating enzyme Usp44”
July 2014
Oral presentation at the Global Research Laboratory Program meeting, Seoul, South Korea.
“Target identification of deubiquitinating enzymes”
September 2009
Poster presentation at “The Spindle Checkpoint as an Oncology Target” meeting, Glasgow, UK.
“Genetic and Biochemical analysis of Cdc14 proteins in vertebrate cells”
December 2008
Poster presentation at the ASCB Annual Meeting, San Francisco, CA.
“Role of Cdc14 proteins in vertebrate cells”
Cell biology: Culture of mammalian cancer cells, T cells, human and mouse embryonic stem cells in 2D and 3D systems; stem cell differentiation; stem cell reprogramming from primary cells; lentivirus production and purification; lentivirus transduction; transfection; multicolor flow cytometry; FACS; immunofluorescence; fluorescence in situ hybridization (FISH); epifluorescence and confocal microscopy; high throughput automated cell-based imaging; cytotoxicity and cell proliferation assays, cytokine release assays, immunosuppression assays.
Biochemistry: protein expression from bacteria, mammalian and insect cells; protein purification by affinity chromatography; SDS-PAGE; western blot; monoclonal and polyclonal antibody production and purification; immunoprecipitation: mass spectrometry; ChIP; in vitro binding assays; enzymatic assays.
Genetics: genome editing via homologous recombination, CRISPR/Cas9; generation of stable cell lines; single cell cloning; characterization of cell clones.
Molecular biology: molecular cloning; PCR, qPCR and RT-PCR; genomic DNA and RNA extraction; DNA electroporation and nucleofection; cDNA synthesis from total RNA; Southern blot; site directed mutagenesis; construction of vectors for gene targeting; design of customized vectors for expression of proteins in E. coli, insect cells and mammalian cells; generation of lentiviral vectors; NGS.
Software: Microsoft Word, Excel, and Power Point; Adobe Photoshop and Illustrator; Tableau; GraphPad Prism; ImageJ, Cell Profiler; BD FACSDiva, FlowJo.
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Resume Overview
School Attended
- ZMBH, Ruprecht-Karls University
- University of Palermo
Job Titles Held:
- Staff Scientist, T Cell Immunology
- Senior Scientist, T Cell Immunology
- Biologist, Genome Editing
- Postdoctoral Research Fellow
Degrees
- Ph.D. : Molecular And Cellular Biology 06/2010
Master of Science : Molecular And Cellular Biology 12/2004
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