Skilled and accomplished cell biologist with 10+ years of experience in academic and industry settings. Strong background in mammalian cell culture and cell-based assay development. Enthusiastic researcher comfortable with working in cross-functional teams, learning cutting-edge technologies and developing novel applications. Eager to join new organization working in a fast-paced, stimulating R&D environment.
Methods for assaying biological cells in a microfluidic device
Patent date: Filed Nov 1, 2019
Patent issuer and number: WO2020092975A2
Proto-Antigen-Presenting Synthetic Surfaces, Activated T Cells, and Uses Thereof
Patent date: Filed Oct 17, 2019
Patent issuer and number: US20200139362A1
Methods for Selection and Generation of Genome Edited T Cells
Patent date: Filed Jun 27, 2019
Patent issuer and number: US20200048606A1
Methods, Systems and Devices for Selection and Generation of Genome Edited Clones
Patent date: Issued Mar 26, 2019
Patent issuer and number: US10239058B2
General functional assay
Patent date: Filed Dec 28, 2018
Patent issuer and number: WO2019133874A1
Antigen-presenting synthetic surfaces, covalently functionalized surfaces, activated T cells, and uses thereof
Patent date: Filed Jul 20, 2018
Patent issuer and number: WO2019018801A1
Tumor Infiltrating Cells Engineered to Express a Pro-Inflammatory Polypeptide
Patent date: Filed Apr 14, 2017
Patent issuer and number: US20170224734A1
Poster presentation at SITC, National Harbor, MD.
"Development of an antigen-presenting bead kit for activation and expansion of human antigen-specific T cells"
Poster presentation at AGBT, Hollywood, FL.
"Massively Parallel Phenotyping, Culturing, Assaying and Sequencing of Single Cells on an OptoFluidic™ Chip"
Oral presentation at the CIRM Stem Cell Fellows Symposium, Berkeley, CA.
“Transcriptional regulation of human embryonic stem cell differentiation by the deubiquitinating enzyme Usp44”
Oral presentation at the Global Research Laboratory Program meeting, Seoul, South Korea.
“Target identification of deubiquitinating enzymes”
Poster presentation at “The Spindle Checkpoint as an Oncology Target” meeting, Glasgow, UK.
“Genetic and Biochemical analysis of Cdc14 proteins in vertebrate cells”
Poster presentation at the ASCB Annual Meeting, San Francisco, CA.
“Role of Cdc14 proteins in vertebrate cells”
Cell biology: Culture of mammalian cancer cells, T cells, human and mouse embryonic stem cells in 2D and 3D systems; stem cell differentiation; stem cell reprogramming from primary cells; lentivirus production and purification; lentivirus transduction; transfection; multicolor flow cytometry; FACS; immunofluorescence; fluorescence in situ hybridization (FISH); epifluorescence and confocal microscopy; high throughput automated cell-based imaging; cytotoxicity and cell proliferation assays, cytokine release assays, immunosuppression assays.
Biochemistry: protein expression from bacteria, mammalian and insect cells; protein purification by affinity chromatography; SDS-PAGE; western blot; monoclonal and polyclonal antibody production and purification; immunoprecipitation: mass spectrometry; ChIP; in vitro binding assays; enzymatic assays.
Genetics: genome editing via homologous recombination, CRISPR/Cas9; generation of stable cell lines; single cell cloning; characterization of cell clones.
Molecular biology: molecular cloning; PCR, qPCR and RT-PCR; genomic DNA and RNA extraction; DNA electroporation and nucleofection; cDNA synthesis from total RNA; Southern blot; site directed mutagenesis; construction of vectors for gene targeting; design of customized vectors for expression of proteins in E. coli, insect cells and mammalian cells; generation of lentiviral vectors; NGS.
Software: Microsoft Word, Excel, and Power Point; Adobe Photoshop and Illustrator; Tableau; GraphPad Prism; ImageJ, Cell Profiler; BD FACSDiva, FlowJo.
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