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Senior Research Scientist Resume Example

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SENIOR RESEARCH SCIENTIST
Professional Summary

Experienced senior scientist with over 15 years of experience in biomedical research field from basic research to disease-related translational studies. Her expertise includes cell biology, molecular biology, immunology, innate immunity of viral recognition and host defense and skin inflammatory diseases. She is always enthusiastic and eager to team success through hard work, attention to detail, excellent organization skills and leadership of encouraging creative work environment.

Skills

Cell culture including cell lines and primary keratinocytes and fibroblasts
Establishment of primary keratinocytes and fibroblasts lines from human skin biopsies
CRISPR/CAS9 gene-editing in primary keratinocytes and fibroblasts
Next generation sequencing approaches and tools
Retroviral and Lentiviral-based gene delivery to generate stable-expression cell lines
Cloning to make mammalian expression constructs
Site-directed mutagenesis
Promoter activity assays including reporter gene assays and CHIP assays
Small siRNA inference technology
DNA and RNA extraction
Various types of PCR
Southern blot, Northern blot, Western Blot and ELISAs
Immunoprecipitation
Recombinant proteins' generation and purification using both eukaryotic and prokaryotic cell system
Human peripheral blood cell isolation and purification
Histology staining and confocal microscopy
Multicolor cytometer
Skin inflammatory and viral infection mice models
Exceptional ability to trouble-shooting experimental problems
Exceptional ability to optimizing experimental system and study strategies
Maintenance of excellent lab records
Comprehensive protocol design to analyze gene function
Human disease oriented translational research protocol design
Implementation of human subjects research policies in translational studies.
Proficiency in office software, prism, endnote, flowjo, imagej, DNA sequence analysis tools.
Proficiency in writing manuscript, standard operating procedures, IRB protocols.
Group leader management

Work History
Senior Research Scientist11/2012 to Current
Company Name – City, State

Conduct research to identify risk factors causing increased viral infections in atopic dermatitis patients. To be specific:

1. Design study protocols to investigate blood and skin specimens collected from atopic dermatitis patients, atopic dermatitis patients with a history of disseminated herpes simplex virus infections (eczema herpeticum) and normal non-atopic healthy individuals.

2. Design in vitro and in vivo experimental systems to investigate the roles of candidate genes, genetic variants and pathways in the pathogenesis of atopic dermatitis and eczema herpeticum using a variety of cellular, molecular and immunological approaches.

3. Write scientific manuscripts and grant applications to apply for fundings from NIH agency and pharmaceutical companies.

4. Train and supervise technicians in the laboratory to conduct projects.

  • Planned and conducted experiments for translational studies of atopic dermatitis.
  • Wrote research papers, reports, reviews and summaries regarding atopic dermatitis etiologies.
  • Supervised junior staff, including technicians and junior scientists.
  • Collected samples and carried out fieldwork.
  • Disseminated research findings to various personnel in different formats, including white papers, presentations and spreadsheets.
  • Validated incoming data to check information accuracy and integrity while independently locating and correcting concerns.
Instructor08/2007 to 11/2012
Company Name – City, State

Conduct research to investigate atopic dermatitis.

1. Investigated the roles of S100 proteins in keratinocytes' host defense against viccinia virus infection.

2. Investigated the roles of specific protein 1 in keratinocytes' biology and host defense aganist herpes simplex virus and vaccinia virus infection.

3. Investigated the effects of staphylococcus aureus virulent factors in keratinocyte viral infections.

4. Wrote scientific manuscripts, presented scientifc data in American Academy of Allergy, Asthma and Immunology annual meetings.

Regular Fellow05/2005 to 07/2007
Company Name – City, State

1. Investigated different formats of TRAIL agonists in killing cancer cells with different TRAIL receptor mutations found in human cancer patients.

2. Investigated different chemotherapy drugs in overcoming cancer cells' resistance to TRAIL induced apoptosis.

Research Associate07/2000 to 05/2005
Company Name – City, State

Conducted research to identify novel genes involved in TRAIL and TLR signaling pathways.

1) Utilized retroviral-based mammalian expression libraries to identify genes that confer resistance to TRAIL-induced apoptosis.

2) Utilized retroviral-based mammalian expression libraries and identified that Marco was the unique receptor of a lung soluble protein, Uteroglobin- related protein 1.

3) Identified the interaction partners of an adaptor protein TIRP (TICAM2) in TLR4 signaling pathway.

4) Made and analyzed the phenotypes of RELT transgenic mice.

Education
M.D.: Clinical Medicine07/1992Hunan Medical University- City
Master of Science: Pathology07/1995Hunan Medical University- City
Ph.D.: Pathophysiology07/2000Central South University- City
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Resume Overview

School Attended

  • Hunan Medical University
  • Central South University

Job Titles Held:

  • Senior Research Scientist
  • Instructor
  • Regular Fellow
  • Research Associate

Degrees

  • M.D. : Clinical Medicine 07/1992
    Master of Science : Pathology 07/1995
    Ph.D. : Pathophysiology 07/2000

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