Senior Research Scientist Resume Example

Cell culture including cell lines and primary keratinocytes and fibroblasts
Establishment of primary keratinocytes and fibroblasts lines from human skin biopsies
CRISPR/CAS9 gene-editing in primary keratinocytes and fibroblasts
Next generation sequencing approaches and tools
Retroviral and Lentiviral-based gene delivery to generate stable-expression cell lines
Cloning to make mammalian expression constructs
Site-directed mutagenesis
Promoter activity assays including reporter gene assays and CHIP assays
Small siRNA inference technology
DNA and RNA extraction
Various types of PCR
Southern blot, Northern blot, Western Blot and ELISAs
Immunoprecipitation
Recombinant proteins' generation and purification using both eukaryotic and prokaryotic cell system
Human peripheral blood cell isolation and purification
Histology staining and confocal microscopy
Multicolor cytometer
Skin inflammatory and viral infection mice models
Exceptional ability to trouble-shooting experimental problems
Exceptional ability to optimizing experimental system and study strategies
Maintenance of excellent lab records
Comprehensive protocol design to analyze gene function
Human disease oriented translational research protocol design
Implementation of human subjects research policies in translational studies.
Proficiency in office software, prism, endnote, flowjo, imagej, DNA sequence analysis tools.
Proficiency in writing manuscript, standard operating procedures, IRB protocols.
Group leader management