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research scientist 1 resume example with 8+ years of experience

Jessica Claire
  • , , 609 Johnson Ave., 49204, Tulsa, OK 100 Montgomery St. 10th Floor
  • H: (555) 432-1000
  • C:
  • resumesample@example.com
  • Date of Birth:
  • India:
  • :
  • single:
Summary
  • Research Scientist with 8 years combined experience in designing, conducting, and managing full scale research projects involving in vivo, ex vivo, and in vitro studies in the field of Immunobiology, Biochemistry, and Cell Biology.
  • Demonstrated a record of research success in the field of diabetes and infectious diseases. A skilled Immunobiologist trained in ABSL2, BSL2, SOPs, ISO, GLP documentations. Have experience in GLP lab/instrument and electronic lab notebook management with strong communication skills.
  • Experienced in data analysis, grant writing, presentation, with strong managerial, and leadership skills. Have trained and mentored graduated students and technicians.
Skills
  • Method Validation
  • Research Program Planning
  • Laboratory Equipment Functions
  • Biochemical and Molecular Analysis
  • Cell culture and Animal Studies
  • Statistical Methodology
  • Data analysis
  • Corrective Actions
  • Assay development
  • Immunoassay
  • Result Interpretation
  • Research and Publication
  • Good Laboratory Practice (GLP)
  • Grant & Paper Writing
  • Experiment Design
  • Bioinformatics
  • Data Collections
  • MS office
Experience
Research Scientist 1, 08/2019 - Current
Northwest Hospital Downtown, WA,
  • Research Focus: Investigating the crosstalk between macrophages, platelets, and other immune cells of heart, liver, and systemic circulations in the development of hyper coagulability in Chagas disease
  • Job Responsibilities
  • Conceptualize assays for designing the experiments to support ongoing NIH-funded research projects
  • Successfully purify immune cells from blood and other organ tissues
  • Purify cardiomyocytes and hepatocytes from heart and liver, respectively
  • Perform routine maintenance of various types of cell lines (primary and transformed cells) and T.cruzi parasites in the invitro culture system
  • Able to design multi color antibody panels for flow cytometry and multiplex immunohistochemistry
  • Perform flowcytometry, multiplex and conventional immunohistochemistry and analyze data to probe different population of immune cells in organ tissues, in vivo circulation system and invitro cell cultures
  • Develop assays to detect biomarkers for Chagas disease mediated risk assessment
  • Successfully perform Western blot, ELISA, ELISPOT, Bio plex (23/26 plex), and chemiluminescent assays to quantify the expression and release of pro and anti-coagulation proteins, inflammatory markers, various transcription factor activity in various tissue samples and cell culture supernatants
  • Design various primers, and antibody targets for the successful run of Chromatin Immunoprecipitation-PCR, Immunoprecipitation-Western blot, RT-qPCR in identifying binding of transcription factors to DNA proteins /promoter region of DNA, and gene expression pattern for chemokines, cytokines and blood coagulation factors
  • Perform Martius Scarlet Blue, H&E, and Masson Trichrome stain in identifying connective tissue and vascular pathology development in Chagas disease
  • Curate and analyze data in Graph pad, SPSS, flowjo, STATA, image J, and Halo image analysis software
  • Using Bioinformatics, analyze genomic data and ChIP Sequence data from the UCSC genome browser and encode portal
  • Develop signaling pathway images in Bio Render software
  • Optimize and troubleshoot experiments, write research protocols, technical reports, manuscripts, and grants
  • Provide technical expertise to the laboratory staff, monitor work quality and supervise graduate students and technicians
  • Maintain laboratory reagent inventory along with the proper management of lab electronic notebook, and data record system
  • Identify the opportunities to improve laboratory work plan and processes
  • Accomplishments
  • Successfully completed one IHII (UTMB) funded pilot grant program
  • Conceptualized experimental design, generated preliminary data and contributed 90% in the writing of NIH R21 grant application on platelet activation and thromboinflammation in Chagas disease
  • The new research direction got a 20-impact fundable score from the NIH grant review committee
  • Successfully detected human blood biomarkers and their prognostic performance in Chagas disease
  • Developed and implemented protocols for Martius Scarlet Blue stain for staining fibrin clusters in the histological section of brain and heart
  • Productive research work resulted in publications in two high impact journals (Frontiers of Immunology and Microbiology Spectrum).
Post-Doctoral Fellow, 03/2018 - 08/2019
Rutgers University New Brunswick, NJ,
  • Research focus: Delineated the molecular mechanisms by which macrophages may contribute to chronic inflammation and cardiac fibrosis in Chagas disease
  • Purified extracellular (EVs) from plasma and in vitro cell culture supernatants
  • Performed molecular characterization of EVs by using nanoparticle tracking analysis, qPCR, conventional PCR, western blot and flow cytometry methods to demonstrate that EV containing DNA signal proinflammatory macrophage activation
  • Designed various primers, antibody targets and developed assays to detect the efficacy of PARP1 and cGAS inhibitor drugs in arresting proinflammatory activation of macrophages in response to T.cruzi infection
  • Performed conventional dual antibody-based immunohistochemistry (Impress duet) and histological H&E staining to probe the immune cells and inflammation in heart tissue of animals infected with T. Cruzi
  • Successfully transfected macrophages with a reporter vector and constitutively expressing Renilla luciferase vector
  • Performed dual Luciferase (Firefly-Renilla) assay to detect NFkB activity in macrophages by measuring luminescence, using a Glomax 96 microplate luminometer
  • Demonstrated differentiation of human cardio fibroblasts to myofibroblasts during T.cruzi infection by immunofluorescent staining and fluorescent microscopy based image analysis of the cells
  • Performed gelatin zymography, western blot, qPCR array, and ELISA to detect macrophage expression and release of matrix metalloproteinases and cytokines
  • Analyzed data and images in Graph pad, SPSS, image J software
  • Optimized and troubleshot experiments, wrote manuscripts, research protocols and reports for NIH funded RO1 grant
  • Conclusion: The research outcome showed chemical inhibition of the enzyme PARP-1 and cGAS offers a potential therapeutic target for mitigating extracellular vesicles driven inflammation in Chagas disease
  • Accomplishments
  • Standardized nano particle tracking analysis for EV characterization, which able to track the morphology of 90% circulating EVs in sample
  • Developed immunohistochemistry and immune fluorescence technique for the immune staining of cardiac immune cells and cardio fibroblasts for the first time in the laboratory to monitor the development of cardiac fibrosis in Chagas disease
  • Productive research work resulted in two high impact manuscripts published in two outstanding journals (Plos Pathogens and mBio journal).
Clinical Laboratory Scientist, 07/2015 - 02/2018
Common Spirit Knoxville, IA,
  • Demonstrated critical thoughts and root cause analysis skills to diagnose poor instrument performance and troubleshot all malfunctions
  • Interpreted critically alert clinical sample values and anomalous laboratory results
  • Designed, standardized, and validated different immunoassays by verifying the performance of different calibrators, and controls (inter assay and intra assay) in closed and open setup-based immunoassay instruments
  • Utilized fully automated biochemistry and electrolyte analyzers, ELISA and electro chemiluminescent immunoassay, and ion exchange HPLC system to increase patient sample throughput and to reduce report turnaround time
  • Conducted laboratory internal audits, developed SOPs for standardized test procedures, maintained GLP documents, and quality standards as per ISO15189, NABL, and NABH guidelines
  • Ensured all the work protocols being followed as per standard operating procedures
  • Supervised and directed the daily laboratory activities with the proper management of the laboratory electronic notebook
  • Conducted training programs and workshops to train laboratory scientific personnel and technicians for professional and scientific skill development
  • Worked on ‘Roche Diagnostics' funded project to evaluate dengue fever hemorrhagic risk assessment
  • Conceptualized and conducted several clinical trials and research
  • Worked with medical directors, clinical operations, and other groups to conduct clinical reviews of study data
  • Accomplishments
  • Effectively generated reliable patients results by ensuring proper quality of the testing system with minimal error and shortened patient report turnaround time by 2 hours
  • Successfully managed a team of 18 members including technicians, quality managers and research assistants
  • Efficient contribution to the clinical sample testing system resulted in getting accreditation from National Accreditation Board for Testing and Calibration Laboratories (India), during an internal audit conducted in 2016 and 2017
  • Successfully completed Roche Diagnostics funded research project in detecting the risk of Dengue fever mediated hemorrhagic manifestations in India
  • The study outcome resulted in publication in a high impact factor journal (Cytokine journal).
Research Associate, 07/2014 - 06/2015
Leidos Holdings Inc. Laconia, NH,
  • Research focus: Defined the inhibitory role of hyperlipidemia mediated increased formation of advanced lipoxidation end products (ALEs) and related adducts on erythrocyte G6PD and antioxidant enzymes in diabetic retinopathy
  • Purified leukocyte free packed erythrocytes from human blood samples
  • Successfully performed spectrophotometer and spectrofluorometer based enzymatic immunoassays to measure the activity of erythrocyte antioxidant enzymes, erythrocyte pyridine coenzymes (NADP, NADPH) and G6PD
  • Detected serum levels of cholesterol and other lipoproteins using a fully automated biochemistry analyzer (Roche Cobas 6000)
  • Detected serum ALEs and lipid adducts using indirect and sandwich ELISA
  • Reviewed study results, analyzed data in Graph pad, wrote manuscript, and prepared project report for ICMR funded projects.
Education and Training
PhD: Biochemistry And Immunobiology, Expected in 06/2014
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Calcutta University - Kolkata, India,
GPA:
Status -
MS: Biochemistry, Expected in 06/2009
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Vidyasagar University - West Bengal, India,
GPA:
Status -
BS: Medical Laboratory Science, Expected in 06/2007
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Vidyasagar University - West Bengal, India,
GPA:
Status -
Additional Information
  • Additional Information , Outstanding researcher award in Immunology: Issued by Chamber of Asia Research Awards, Asia’s Science Technology and Research Awards (ASTRA 2023), Tamil Nadu, India. Guest speaker award: Guest speaker award issued by Euro Obesity and Endocrine Congress Society · Euro Obesity and Endocrinology Congress, Sep 2017, Zurich, Switzerland. Young scientist travel grant award: Issued by Laboratory Medicine Congress Exhibition, Oct 2016, Seoul, South Korea.

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Resume Overview

School Attended

  • Calcutta University
  • Vidyasagar University
  • Vidyasagar University

Job Titles Held:

  • Research Scientist 1
  • Post-Doctoral Fellow
  • Clinical Laboratory Scientist
  • Research Associate

Degrees

  • PhD
  • MS
  • BS

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