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Postdoctoral Research Associate Resume Example

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Jessica Claire
  • , , 100 Montgomery St. 10th Floor
  • H: (555) 432-1000
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  • resumesample@example.com
  • Date of Birth:
  • India:
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Summary

Ambitious, innovative and results oriented biomedical research scientist having 3 years of postdoctoral research experience with wide expertise in protein structure biology, biochemistry and biophysics

  • Experienced in understanding molecular level interactions between antigens and neutralizing antibodies elicited post vaccination
  • Experienced in understanding molecular interactions between proteins involved during trafficking of viruses in host cells
  • Technically proficient with a diverse background in various human diseases like TB, HIV, HPV, SARS-COV2 and malaria
  • Ability to successfully meet scientific challenges and execute diverse scientific projects both individually and within teams
  • Experience with management of laboratory budgets, problem solving and troubleshooting, training and supervision of junior and technical staff
Skills

Technical Skills:

  • Molecular Biology: DNA isolation, purification and quantification, PCR, DNA cloning, Site-directed mutagenesis
  • Protein Biochemistry: Expression and purification of soluble and membrane proteins using E.coli and mammalian expression systems. Expression and purification of glycoproteins.
  • Protein purification Techniques: Chromatography (gel filtration, ion exchange, affinity, HPLC), dialysis.
  • Protein characterization using circular dichroism, DLS, Western blot, SDS PAGE, Native PAGE.
  • Characterizing protein-ligand/ antigen-antibody interactions using Bio-Layer Interferometry (BLI) and ELISA assay.
  • Protein Crystallography: Screening and optimizing crystallization conditions using manual and pipetting robot method, Data collection using home source, and Synchrotron. Data processing using XDS, MOSFLM, HKL2000 packages, Structure solution using SAD and MR methods, Refinement using CCP4i and PHENIX, Model building using Coot and Molecular visualization using Pymol and Chimera
  • Single Particle EM: Preparation and imaging of negative staining EM grids, preparation of cryo-EM grid using Vitroblot and Chameleon, imaging cryo-EM grids using Glacios and Krios, data processing using RELION, cryoSPARC and cisTEM
  • Cell culture: Maintenance of mammalian and insect cell cultures
  • Bioinformatics skills: Molecular biology based software's like Gene runner, SnapGene, Statistical data analysis using excel, Prism- GraphPad
  • Computer skills: Proficient in Microsoft office ( Word, Excel, PowerPoint). Expert in making spreadsheets, slide presentations, inventories, record keeping, lab notebooks, and project reports.

Management Skills:

  • Active in problem solving, troubleshooting and quality control to ensure project success
  • Supervised and managed technical staff and trainees.
  • Participated in lab budget management
  • Assist in ordering reagents, equipments and instruments for the lab

Team skills:

  • Participated in hiring interns, trainees, and postdocs
  • Organized lab social interactions and events
  • Guided and trained technicians, junior PhDs, Masters and summer trainees in the success of their research projects during my PhD. and postdoc career
Experience
Postdoctoral Research Associate, 01/2021 to Current
Rutgers University New Brunswick, NJ,
  • Expressed full length SARS COV2 spike protein and GPCR-like membrane proteins using mammalian expression system and purified these protein using affinity and size exclusion chromatography.
  • Studied molecular level interaction of SARS COV2 spike protein and host proteins using BLI and X-ray crystallography.
  • Deposited the related PDB's structures in PDB database.
  • Cloned, expressed and purified interacting host partners for SARS COV2 spike protein using E.coli expression system.
  • Advanced in the technical skills of Cryo-EM by understanding the glycosylation pattern of SARS COV2 spike protein.
  • Mentored fellows, trainees and juniors to execute assigned tasks with validity.
  • Collected, input, analyzed and presented results of research.
  • Searched pertinent scientific literature to support efforts with empirical data.
  • Kept laboratory and office environments clean, tidy and well-stocked with supplies.
  • Oversaw media preparation, maintenance and quality control analysis of reagents.
Postdoctoral Research Fellow, 07/2018 to 12/2020
University Of Kansas Medical Center Osawatomie, KS,
  • Solved the structures of vaccine- and natural infection-elicited antibodies from different B cell compartments as apo and in complex with HPV16 (Human papilloma virus) L1 protein in order to understand the differences and the type of immune response elicited at the molecular level.
  • Did structural characterization of PfMSP1-19 in complex with antibodies to determine novel epitopes of vulnerability and guide the design of blood stage vaccine candidate for Plasmodium falciparum.
  • Structural characterization of one of the antibodies GM9_TH8, specific for HIV envelope glycoprotein, explaining the effect of somatic hypermutation in the complementary determining regions on the neutralizing capacity of lineage of antibody.
  • Performed characterization of the epitopes of HPIV3 Pre-fusion protein recognized by neutralizing antibodies using negative staining and cryo-EM structures.
  • Analyzed and fine tuned the data from structural and biochemical findings and reported the data in research publications.
  • Helped in writing grants and manuscripts.
Ph.D. Fellow, 07/2012 to 06/2018
Institute Of Microbial Technology, (JNU) City, STATE,
  • Implemented strategies to express and purify a difficult to express hypothetical transcriptional regulator protien from Mycobaterium tuberculosis using E.coli expression system.
  • Designed and performed experiments to characterize the function of this hypothetical protein using biochemistry based technique like EMSA (Electrophoretic Mobility Shift Assay). Characterized the stability of protein using Circular Dichroism. Characterized the structure of protein using SAD phasing method.
  • Performed the structural characterization of protein (Protein Kinase A) for a collaborative research group and revealed the presence of ordered activation loop in the protein that showed the inactive form of protein.
  • Participated with the research team to perform enzyme kinetic activity assay to determine the affinity of enzyme with its substrate and small molecule inhibitors.
  • Provided mentorship and supervision to summer trainees in teaching cloning, expression, purification and crystallization of protein.
  • Constructed academic papers, presentations and grants to assist research study efforts.
  • Collaborated with colleagues to discuss research programs and methodologies to influence leading edge practices.
M.Sc. Scholar, 07/2009 to 06/2011
Banasthali University City, STATE,
  • Qualified entrance exam to get scholarship from Department of Biotechnology (DBT), Govt. of India during M.Sc. Course
  • Leveraged Microsoft Word and PowerPoint to prepare reports, presentations and articles.
  • Leveraged excellent project management and leadership skills at school to coordinate group activities, complete projects and expand knowledge.
  • Obtained and organized files and research papers to make important documents easier to find.
Education and Training
Ph.D.: Protein Biochemistry And Structure Biology, Expected in 06/2018
to
Jawahar Lal Nehru University (IMTECH, Chandigarh) - New Delhi,
GPA:
  • Thesis: Structural and Biochemical Characterization of an Essential Transcriptional Regulator from Mycobacterium tuberculosis
  • Completed coursework in Biophysics, Cell Biology, Genetics and Bioinformatics
  • Received 5 years research Fellowship from CSIR-UGC in India to pursue Ph.D.
  • Ranked in Top 5% of class
  • Member of Indian Biophysical Society
Master of Science: Biotechnology, Expected in 06/2011
to
Banasthali University - Jaipur, Rajasthan, India,
GPA:
  • Ranked in Top 10% of class
  • Received scholarship from Department of Biotechnology (DBT), India during the program
Bachelor of Science: Biotechnology, Expected in 06/2009
to
Banasthali University - Rajasthan, India,
GPA:
Websites, Portfolios, Profiles
  • https://www.linkedin.com/in/suruchi-Claire-98761615b/
  • https://scholar.google.com/citations?user=Y4advMsAAAAJ&hl=en

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Resume Overview

School Attended
  • Jawahar Lal Nehru University (IMTECH, Chandigarh)
  • Banasthali University
  • Banasthali University
Job Titles Held:
  • Postdoctoral Research Associate
  • Postdoctoral Research Fellow
  • Ph.D. Fellow
  • M.Sc. Scholar
Degrees
  • Ph.D.
  • Master of Science
  • Bachelor of Science