Qualified by over six years of research experience in molecular biology, microbiology, immunology and virology. I developed high-throughput gene expression and sequencing assays using RT-qPCR, RNA-seq, SDS-PAGE and 2D gel electrophoresis to establish scientific models in an international, team oriented environment. Additionally, I worked with military and industry leaders to develop novel, PCR-based detection methods (patent pending) for rapid, point-of-care diagnostics of highly infectious diseases (e.g. Plague, Anthrax and Tularemia) .
Investigated gene expression and characterization of secondary metabolite production in Yersinia pestis. Developed a novel diagnostic technique based on qPCR for the safe detection of highly pathogenic bacteria. Established an institute wide protocol for isolation of nucleic acids and quality control standards for RT-qPCR based on MIQE guidelines. Submitted data to peer reviewed journals and applied for government grants.
Pertinent skills: Assay setup and design using - RT-qPCR, RNA-seq, SDS-PAGE, 2D gels, MALDI-TOF MS, genome mining, bioinformatic analysis, diagnostics, nucleic acid extraction, quantification and quality control.
I worked in a team environment to develop a NeuroAIDS and retroviral drug resistance model by characterizing HIV strains exposed to ART and psychostimulants. Gene targets were studied from brain tissue cultures using RT-qPCR and viral antigen detection was performed from supernatants using ELISA.
Pertinent skills: ELISA, cDNA synthesis, RT-qPCR, cell culture, flow cytometry, antibody staining, fluorescent and confocal microscopy, gel electrophoresis
Expected defense date: Jan. 2014.
Wieser, A., Schneider, L., Jung, J. and Schubert, S. (2001). MALDI-TOF MS in microbiological diagnostics - identification of microorganisms and beyond. Applied Microbiology and Biotechnology 93, 965-974.
Rakin, A., Schneider, L. and Podladchikova, O. (2012). Hunger for iron: the alternative siderophore iron scavenging systems in highly virulent Yersinia. Frontiers in Cellular and Infection Microbiology 2, 1-7.
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