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Lab Manager Resume Example

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LAB MANAGER
Summary
Research Scientist with a strong immunology and stem cell background coupled with 12 years of innovative assay development skills and 4 years of GCLP lab managerial expertise.
Skills
Scientific Expertise- Sterile techniques, primary and cell-line culture, characterization by flow cytometry, neutralization assays, ELISpot, ADCP, basic HPLC, cytospinning, sonication, cell isolation and sorting, sectioning using microtome, spectrophotometry, X-ray film development , microplate reading and Smartspec. Scientific Publications- 18 PubMed indexed publications in reputed journals CTL Immunospot , Viacount and Guava Expressplus, Microsoft Office, Adobe Photoshop, Live Cycle Designer and DC.
Work History
Department of Viral Pathogenesis
Department of Hematology & Oncology
Accomplishments
  • Graduate Teaching and Research Assistant, Department of Bioengineering, University of Texas at Arlington, Arlington, Texas, USA (June 2005- May 2006).
  • Designed teaching aides to help students understand the concepts of Tissue Engineering.
  • Developed a student friendly lab module covering the basics of cell culture, scaffolding, protein coatings, histological procedures and mammalian cell culture techniques.
  • In-vitro culturing and growth rate measurement of Human Mesenchymal Stem Cells.
  • Prepared slides by cytospin deposition and performed H&E staining Graduate Thesis Optimization Of Methods For In-vitro Expansion And Cryopreservation Of Mammalian Stem Cells.
  • Compared the serum-free versus serum rich media to arrive at the optimum serum concentration.
  • Recommended the addition of HB-EGF as a growth stimulant.
  • Researched the effects of cryoprotectants and freezing techniques.
  • Evaluated various methods to improve the viability of cells in liquid Nitrogen.
  • Studied the effect of cell density in the cryovial on the survival rate.
  • Arrived at the best possible cryopreservation technique for mammalian cell lines and stem cells Under-Graduate Thesis Intelligent Cane Using Mixed Signal Processing (MSP).
  • Conducted surveys to improve the ordinary white cane used by the visually challenged.
  • Eliminated bulky electronic interfaces by using an MSP chip thereby making it more affordable and ergonomic than other smart canes.
  • Designed a successful collision prevention alarm system to detect obstacles.
  • Improved user-safety by incorporating water detection and smoke detection modules to the cane.
Experience
Lab Manager Nov 2013 to Current
 Center for Virology and Vaccine research, Beth Israel Deaconess Medical Centre/Harvard Medical University － Boston, USA, Massachusetts
  • Managing and advancing a GCLP certified pre-clinical Non-Human Primate HIV vaccine research lab.
  • Hiring, training and mentoring junior researchers.
  • Planning and designing standard operating procedures (SOPs) for laboratory equipment and immune assays to maximize productivity and accountability.
  • Collaborating with research teams nation-wide to standardize critical assays.
  • Assisting the PI and the Program Manager with budgeting and grant writing.
  • Ensuring institutional biosafety compliance as the lab biosafety officer.
  • Scaled-up hybrid SHIV virus challenge stocks of varying infectivity for pre-clinical trials.
  • Developed new assays to enhance the lab portfolio - B cell ELISpot, viral inhibition assay.
  • Analyzed multi-dimensional data generated from multi-faceted studies.
  • Cell sorting using AutoMACS for downstream applications.
  • Generate Batch Record templates for all the standardized assays for stream-lined record maintenance.
Sr. Research Associate-Assistant II Nov 2006 to Nov 2013
 Beth Israel Deaconess Medical Center/Harvard Medical University － Boston, USA, Massachusetts
  • Tested efficiency of SIV/HIV vaccination regimens using ELISpot and Flow Cytometric assays on mice, non-human primates and humans.
  • Mononuclear cell extraction from peripheral blood and mucosal biopsy specimens for immunologic evaluation.
  • Evaluated vaccine efficacy by cytokine characterization using intercellular cytokine staining.
  • Economized in-lab monoclonal antibody production cost (200 times more cost-efficient than the comparable commercial product).
  • Neutralization antibody profile generation for longitudinal clinical trial timepoints.
  • B-lymphoblastoid cell line generation from primate and human blood cells for immortalized cell culture.
  • Quality Controlled all the above mentioned assays by inventory control and bridge testing between consecutive lots.
Research Assistant May 2006 to Oct 2006
 Veterans Affairs Hospital － Dallas, USA, Texas
  • Evaluated chronic myelogenous leukemia medications by suspension and adherent cell culture techniques.
  • Performed cell proliferation assays to understand the pharmacokinetics of treatment options.
  • Protein estimation and Western blotting on bone marrow stem cells to understand drug dosage effects.
Education and Training
Master of Science, Biomedical Engineering May 2006 University of Texas － Arlington, USA Biomedical Engineering 3.22 Optimization Of Methods For In-vitro Expansion And Cryopreservation Of Mammalian Stem Cells
Bachelor of Engineering, Electronics & Communication May 2003 Bharathiar University － Coimbatore, India Electronics & Communication Distinction Intelligent Cane Using Mixed Signal Processing (MSP)
Publications
Bradley T, Pollara J, Santra S, Vandergrift N, Pittala S, Bailey-Kellogg C, Shen X, Parks R, Goodman D, Eaton A, Balachandran H, Mach LV, Saunders KO, Weiner JA, Scearce R, Sutherland LL, Phogat S, Tartaglia J, Reed SG, Hu SL, Theis JF, Pinter A, Montefiori DC, Kepler TB, Peachman KK, Rao M, Michael NL, Suscovich TJ, Alter G, Ackerman ME, Moody MA, Liao HX, Tomaras G, Ferrari G, Korber BT, Haynes BF. Pentavalent HIV-1 vaccine protects against simian-human immunodeficiency virus challenge. Nat Commun. 2017 Jun 8;8:15711. doi: 10.1038/ncomms15711. Pardi N, Hogan MJ, Pelc RS, Muramatsu H, Andersen H, DeMaso CR, Dowd KA, Sutherland LL, Scearce RM, Parks R, Wagner W, Granados A, Greenhouse J, Walker M, Willis E, Yu JS, McGee CE, Sempowski GD, Mui BL, Tam YK, Huang YJ, Vanlandingham D, Holmes VM, Balachandran H, Sahu S, Lifton M, Higgs S, Hensley SE, Madden TD, Hope MJ, Karikó K, Santra S, Graham BS, Lewis MG, Pierson TC, Haynes BF, Weissman D. Zika virus protection by a single low-dose nucleoside-modified mRNA vaccination. Nature. 2017 Mar 9;543(7644):248-251. doi: 10.1038/nature21428. Epub 2017 Feb 2. Asmal M, Lane S, Tian M, Nickel G, Venner C, Dirk B, Dikeakos J, Luedemann C, Mach L, Balachandran H, Buzby A, Rao S, Letvin N, Gao Y, Arts EJ. Pathogenic infection of Rhesus macaques by an evolving SIV-HIV derived from CCR5-using envelope genes of acute HIV-1 infections. Virology.
Skills
Adobe Photoshop, basic, budgeting, cell culture techniques, cell culture, clinical trials, DC, designing, downstream, film, grant writing, Hiring, HPLC, inventory control, laboratory equipment, Managing, mentoring, Microsoft Office, Optimization, Publications, Quality, reading, research, Scientific, sorting, vaccine, Western blotting, X-ray
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Resume Overview

Companies Worked For:

  • Department of Viral Pathogenesis
  • Department of Hematology & Oncology
  • Center for Virology and Vaccine research, Beth Israel Deaconess Medical Centre/Harvard Medical University
  • Beth Israel Deaconess Medical Center/Harvard Medical University
  • Veterans Affairs Hospital

School Attended

  • University of Texas
  • Bharathiar University

Job Titles Held:

  • Lab Manager
  • Sr. Research Associate-Assistant II
  • Research Assistant

Degrees

  • Master of Science , Biomedical Engineering May 2006
    Bachelor of Engineering , Electronics & Communication May 2003

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