Highly motivated, hard-working, skilled multitasker with excellent communication and problem-solving biochemist and structural biologist with 10+ years of extensive experience in protein sciences in support of therapeutics and drug development by performing biochemical, biophysical, enzymatic, and structural analysis for drug candidates. Developed a wide range of quantitative, bio-analytical methods for the understanding and evaluation of protein–ligand interactions for several therapeutic targets such as epigenetic enzyme PRMT5. Actively involved in early-stage drug discovery from hit identification, target validation, lead optimization to mechanism of action studies. Current research interests include drug discovery through multi-disciplinary techniques especially protein–ligand binding kinetics/affinity analysis and in vitro biological evaluation of drug candidates in the therapeutic product development pipeline and structure-based drug design using cryo-EM and/or X-ray crystallography.
1. Tuberculosis (TB), one of the oldest known human diseases, is still one of the major causes of human mortality. Although many medicines are available for TB, its therapy takes prolonged time before complete cure. For this very reason, better inhibitors of the mycobacterium tuberculosis have been persistently pursued. We found that exogenously added mycobacterium phosphoserine phosphatase (MtSerB2) induces microtubule rearrangements in THP-1 cells, which are progenitor cells for macrophage-like cells. My graduate studies demonstrated that the phosphatase activity is tightly correlated to the elicited microtubule rearrangements (https://doi.org/10.1371/journal.pone.0115409).
2. HIV-1 Nef is an important accessory protein contributing to multiple distinct functions of the virus, such as immune evasion, virion infectivity, and support for viral replication and survival. These functions are regulated by the interactions of Nef with more than 30 different partner proteins in membrane-anchored or cytoplasmic state and is puzzling how such a small protein (∼27 kDa) can control multiple important functions. We discovered how changes of the oligomeric state of HIV-1 Nef can help it to distinguish between its protein partners by showing the molecular details underlying a novel dimer-tetramer transition of Nef, a critical step for its interaction with its partners (https://doi.org/10.1371/journal.pone.0026629).
1. Mohammed E. Sayed, Ao Cheng, Gaya P. Yadav, Andrew T. Ludlow, Jerry W. Shay, Woodring E. Wright, and Qiu-Xing Jiang; Catalysis-dependent inactivation of human telomerase and its reactivation by intracellular telomerase-activating factors (iTAFs). J. Biol. Chem. June 11, 2019.
2. Gaya P. Yadav, Hui Zheng, Qing Yang, Lauren G Douma, Linda B Bloom, and Qiu-Xing Jiang; Secretory granule protein chromogranin B (CHGB) forms an anion channel in a membrane. Life Science Alliance, Sept 2018, 1(5) e201800139; DOI:10.26508/lsa.201800139.
3. Yadav GP, Current Dilemma on Granin Proteins: Proteins involved in various cellular functions without known mechanisms. Cell Cellular Life Science J (2017), 2(2):000115.
4. Liu H, Liang C, Kollipara RK, Matsui M, Ke X, Jeong BC, Wang Z, Yoo KS, Yadav GP, Kinch LN, Grishin NV, Nam Y, Corey DR, Kittler R, Liu Q. Mol Cell. (2016) 4; 63(3):420-32. DOI: 10.1016/j.molcel.2016.06.014. Epub 2016 Jul 14.
5. Yadav GP, Shree S, Maurya R, Rai N, Singh DK, et al. Characterization of M. tuberculosis SerB2, an Essential HAD-Family Phosphatase, Reveals Novel Properties. (2014) PLoS ONE 9(12): e115409.
6. Singh P*, Yadav GP*, Gupta S, Tripathi AK, Ramachandran R, Tripathi RK. A novel dimer-tetramer transition captured by the crystal structure of the HIV-1 Nef. PLoS One. 2011; 6(11): e26629. (*Equal author).
7. Vijay Singh, Samiran Hutait, Gaya P. Yadav, Prakas R. Maulik and Sanjay Batra* Unusual retention of isoxazole ring under the influence of 3-(substituted nitrophenyl)-2-isoxazoline during catalytic-hydrogenation of isoxazoline substituted isoxazole systems. Journal of Heterocyclic Chemistry, 2009, 46,762-769.
8. Uma Sharan Singh, Ravishankar nainavat, Gaya P. Yadav, Geetika kharkwal, Anila Dwivedi, Govind Keshri, M.M Singh, P. R Maulik, and K. Hajela. Synthesis and structure-guided evaluation of estrogen agonist and antagonist activities of some new tetrazolyl indole derivatives, Eur. J. Med. Chem., 2008, 43, 2149-2158.
9. B.K. Singh, Mridul Mishra, Nisha Saxena, G.P. Yadav, P.R. Maulik, M.K. Sahoo, R.L. Gaur, P.K.Murthy, R.P.Tripathi, Synthesis of 2-sulfanyl-6-methyl-1, 4-dihydropyrimidines as a new class of antifilarial agents, Eur. J. Med. Chem., 2008,43,2717-2723.
10. Virender Singh, Gaya P. Yadav, Prakas R. Maulik, Sanjay Batra, Synthesis of substituted 3-methylene-2-pyridones from Baylis–Hillman derivatives and its application for the generation of 2-pyridone substituted spiroisoxazolines. Tetrahedron,2008, 64, 13, 2979-2991.
11. Sudharshan Madapa, Divya Sridhar, Gaya P. Yadav, Prakas R. Maulik and Sanjay Batra. A General Approach to the Synthesis of Substituted Isoxazolo[4,3-c] quinolines via Chalcones. Eur. J. Org. Chem. 2007, 26, 4343–4351.
12. Nag, S. Yadav, G.P. Maulik, P.R. Batra, S. Sodium Hydride Mediated Cascade Reaction towards the Synthesis of 1, 5-Disubstituted Uracil from Cyanamides Derived from Baylis-Hillman Adducts. Synthesis; 2007, 06, 911-917.
13. V.Singh, G.P.Yadav, P. R. Maulik, S. Batra, Studies toward the construction of substituted piperidine-2-ones and pyridine-2-ones from Baylis–Hillman adducts: discovery of a facile synthesis of 5-methyl-4-oxo-6-aryl-3-aza-bicyclo[3.1.0]hexane-1-carboxylates, Tetrahedron,2006, 62, 37, 8731-8739.
14. V.Singh, S. Madapa, G.P.Yadav, P. R. Maulik, S. Batra, Interesting Results of catalytic hydrogenation of 3-(2-Nitrophenyl) isoxazoles and 3-(Nitrophenyl) 4,5-dihydroisoxazoles; Synthesis 2006,12,1995-2004.
15. Roy, Amrendra K.; Pathak, Richa; Yadav, Gaya P.; Maulik, Prakas R., Batra, Sanjay, Neighboring-Group Effect: DBU-Promoted Ring Transformation of Substituted Isoxazoles to Substituted Pyrroles, Synthesis 06, 2006, 1021-1027.
1. Gaya P. Yadav, Mani Annamalai, Clayton Mathews, and Qiu-Xing Jiang. Chromogranin B channel drives normal granule maturation in (neuro) endocrine cells (under review).
2. Gaya P. Yadav, Liang Shi, Hui Zheng and Qiu-Xing Jiang, Cryo-EM structure of a potassium ion channel in the inactivated state (Manuscript under review).
3. Gaya P. Yadav, Mahesh K. Chandak and Qiu-Xing Jiang, Competitive reconstitution reveal low-affinity protein-lipid interactions in the membrane. (Manuscript under preparation).
4. Gaya P Yadav‡, Wei Zhou‡, Xiaojie Zhan, Qiu-Xing Jiang, Chenglong Li, Cryo-EM structure of human PRMT5: MEP50 in complex with novel inhibitor at a resolution of 3.1Å (under review,‡ Equal first Author).
1. Synthesis and Post-Coital Contraceptive of some Tetrazolyl Indole Derivative. Uma Sharan Singh, Ravi Shankar, M.M. Singh, Govind Keshri, Gaya P. Yadav, P.R. Moulik and K. Hajela. Med. Chem. Res. 2007, 15, 306-307.
2. Electron Cryo-Microscopic Study of the type 1 IP3R. Hui Zheng, Gaya P. Yadav, Brian Borkowski, Qiu-Xing Jiang. Biophysical Journal, Volume 102, Issue 3, Supplement 1, 31 January 2012, P110a.
3. The Ion Channel Function of Mouse Chromogranin B. Gaya P. Yadav, Hui Zheng, Qing Yang, Qiu-Xing Jiang. Biophysical journal, 2015, 108, 2, p226a.
4. Chromogranin B serves the long-sought-after anion conductance in regulated secretion. Gaya P. Yadav, Qing Yang and Qiu-Xing Jiang. Biophysical Journal, 2017, Vol. 112, Issue 3, p184a.
1. Gaya P. Yadav and Qiu-Xing Jiang; Recosntituted Membrane Systems for Assaying Membrane Proteins in Controlled Lipid Environments. (Book Chapter in “New Techniques for Studying Biomembranes”,Taylor & Francis Group, April 7, 2020).
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