Research Lab Specialist Resume Example

Proficient Laboratory Researcher with more than 10 years of experience working in academic, hospital and health care industry in collaborative settings. Highly skilled in molecular biology, cell therapy including CAR-T and NK-CAR, Next-gen sequencing etc. Proven expertise in devising and optimizing new assays. Currently, seeking Scientist/Senior Scientist role in industry settings.

Publications

1. Myeloid Sarcoma: The other side of Acute Leukemia.
B.Kahali
[Hematology latest research and clinical advances – Book Chapter]

2. Flavonoids from each of the six structural groups reactivate BRM, a possible cofactor for the anticancer effects of Flavonoids.
B.Kahali*, S.B. Marquez*, K.W. Thompson, J. Yu, S.J. Gramling, L. Liu, A. Aponick, D. Reisman (* - Equal Contribution)
[Carcinogenesis. 2014 Oct:35(10):2183-93]

3. The silencing of the SWI/SNF subunit and anticancer gene BRM in Rhabdoid tumors. B. Kahali, J. Yu, S.B. Marquez, K.W. Thompson, S.Y. Liang, L. Lu, D. Reisman [Oncotarget. 2014 May 30;5(10):3316-32]

4.Identifying targets for the restoration and reactivation of BRM.
B.Kahali, S.J.B. Gramling, S.B. Marquez, K. Thompson, L. Lu, D. Reisman [Oncogene 2014 Jan 30:33(5):653-64] [Awarded the highest impact publication (2012), Department of Medicine, University of Florida]

5. Downregulation of SWI/SNF chromatin remodeling factor subunits modulates cisplatin cytotoxicity. A. Kothandapani, K. Gopalakrishnan, B. Kahali, D. Reisman, S. M. Patrick [Exp Cell Res. 2012 Oct; 318(16): 1973-86.]

6. Evidence that the C-terminal domain (CtD) autoinhibits neural repression byDrosophila E(spl)M8.
B.Kahali, J.E. Kim, U. Karandikar, C.P. Bishop, A.P. Bidwai. [Genesis-2010 Jan;48(1):44-55]

7. Functional Dissection of Timekeeper (Tik) implicates opposite roles for CK2 and PP2A in Drosophila neurogenesis.

E. Kunttas-Tatli, A. Bose, B. Kahali, C.P. Bishop, A.P. Bidwai [Genesis. 2009 Oct;47(10):647-58]

8. On the mechanism underlying the divergent retinal and bristle defects of M8*(E(spl)D) in Drosphila.
B.Kahali, A. Bose, U. Karandiakar, C.P. Bishop, A.P. Bidwai [Genesis. 2009 Jul;47(7):456-68]

9. Drosophila CK2 phosphorylates Hairy and regulates its activity in vivo. B. Kahali, R. Trott, Z. Paroush, R. Allada, C.P. Bishop, A.P. Bidwai [Biochem Biophys Res Commun.2008 Sep 5;373(4):637-42]

10. Drosophila CK2 regulates lateral-inhibition during eye and bristle development A. Bose, B. Kahali, S. Zhang, JM Lin, R. Allada, , U. Karandikar, A.P. Bidwai [Mech Dev. 2006 Sep;123(9):649-64.]

Awards and Honors

2010 - WVU Dissertation Fellowship

2008 - WVU Travel Award (Awarded for presentation at the 49th Annual Drosophila Conference, Houston, TX)

2006 - WVU Travel Award (Awarded for presentation at the 47th Annual Drosophila Conference, San Diego, CA)

2000-2002 National Scholarship Award, Govt. of India.

• Business Foundations Specialization- Wharton Online (University of Pennsylvania)
• The Project Management Course: Beginner to Project Manager (Learn@Forbes)

• Member, Cancer Epigenetics Society, 2017 to Current

Molecular Biology

• Next-gen sequencing - extensive experience in library preparation, target enrichment etc.
• Cell-free DNA (cfDNA)/ Circulating tumor DNA (ctDNA).
• Cell-based therapy including CAR-T and NK cell based treatment.
• Methylation asssay.
• Homologous recombination based DNA manipulations - TALEN, CRISPR/Cas9.
• Chromatin Immunoprecipitation (ChIP) assay.
• PCR including qPCR, RT-PCR and ddPCR.
• Molecular cloning and site-directed mutagenesis.
• Southern Blot.
• RNA isolation and RNA interference based (siRNA and shRNA) gene silencing.
• Protein purification.
• Western Blot (both traditional and eWestern), Immunohistochemistry assay, ELISA.

In vivo Technique

• Murine modeling of Human disease.
• Trained in classical genetics and disease modeling in Drosophila.

Computer Skills

• Flow Jo
• Diva
• Python
• Adobe Illustrator
• Adobe Photoshop
• Graphpad
• DNASTAR
• Microsoft office