Two years of experience in induced pluripotent stem cells. · Experience in primary culture of islets of Langerhans, cardiomyocytes and microglia. · Skilled in microscopy, basic molecular biology techniques and immunoassays · Three years of strong experience in pharmaceutical industry in new drug discovery program with strong knowledge of the target to candidate process which lead molecule reached up to phase two clinical trial. · Excellent interpersonal, communication skills and team working capability which resulted in numerous collaborations and 12 publications. · Simultaneously handled various drug screening projects which reflects high flexibility and proactive with strong analytical and problem-solving skills, ability to prioritize work across a complex range of objectives, and quick adaptability to changes in priority.
Stem Cells: Reprogramming of fibroblast to iPSCs, characterization of iPSCs for pluripotency, neuronal stem cell generation from iPSCs · Cell culture: Culture and maintenance of different cell lines, isolation and purification of rat pancreatic islets and cardiomyocytes · Molecular Biological Techniques: Isolation of DNA & RNA, PCR, cDNA preparation, Gel Electrophoresis, TOPO-TA Cloning, siRNA, Western blot, Immunofluorescence · Biochemical Assays: ELISA, enzyme assays, estimation of biomolecules · Microscopy techniques: 2-Photon laser scanning microscopy, EVOS FL Auto Cell Imaging System · Mitochondrial Physiology: Mitochondrial functionality measurement with Seahorse instrument, isolation of mitochondria, mitochondrial membrane potential measurement by TMRM dye, ROS measurement from mitochondria.
Leadership · Played an important leadership role in the lab for maintaining the cell culture room to ensure coordination between all users, maintaining cleanliness and consumable inventory.
Lead member in organizing scientific conference at the Maharaja Sayajirao University of Baroda.
I undertook responsibilities involving publication of advertisement for the conference, arrangement of accommodation and travel for the guest speakers from the different parts of India.
Postdoctoral FellowUniversity of Maryland, Baltimore － Baltimore, MD
Exploring new targets to inhibit proinflammatory microglia.
Established siRNA delivery method for hard to transfect microglial cells · Co-authored a peer-review publication.
Postdoctoral FellowJohns Hopkins University School of Medicine － Baltimore, MD
Developed induced pluripotent stem and neural stem cells from the blood of Ataxia-Telangiectasia (A-T) patient to study as a cellular model system.
One first author paper and one report published · Successful generation of patient derived stem cell/neuronal stem cells resulted in additional funding of $ 0.1 million.
Research associateSun Pharma Advanced Research Company Limited Gujarat
Played a pivotal role in new chemical entity (NCEs) hit-to-lead discovery program · Performed efficacy studies of anti-histamine, anti-inflammatory and anti-cancer NCEs in various animal models.
Carried out cardiovascular safety pharmacological studies and drug-drug interaction studies of selected NCEs · Directed in vitro and in vivo assay optimization and reproducibility with a focus on cost efficiency and time constraints · Preplanned and executed experiments with intense precision, documentation, and methodology improvement with each iteration · Followed good laboratory practice (GLP) guidelines for experiments and written standard operating procedures (SOPs) Niraj M Bhatt, Ph.D · Trained and supervised two junior staff.
Was a Key player on preclinical studies of one of the anti-histamine compound that reached phase two clinical trial in 2011.
Promoted to research associate position in a short span of three years of tenure.
Education and Training
Doctor of PhilosophyThe Maharaja Sayajirao University of Baroda － VadodaraIndia
Masters of Business Administration: Health Care ServicesSikkim Manipal University Distance Education － VadodaraIndiaHealth Care Services
Masters of Science: BiochemistryThe Maharaja Sayajirao University of Baroda － VadodaraIndiaBiochemistry
Bachelors of Science: Botany, Zoology, ChemistryThe Maharaja Sayajirao University of Baroda － VadodaraIndiaBotany, Zoology, Chemistry
Selected publications 1. Jaber, S. M., Bordt, E. A., Bhatt, N. M., Lewis, D. M., Gerecht, S., Fiskum, G., & Polster, B. M. (2017). Sex differences in the mitochondrial bioenergetics of astrocytes but not microglia at a physiologically relevant brain oxygen tension. Neurochem Int. doi:10.1016/j.neuint.2017.09.003 2. Srivastava, A., Bhatt, N. M., Patel, T. P., Dadheech, N., Singh, A., & Gupta, S. (2016). Anti-apoptotic and cytoprotective effect of Enicostemma littorale against oxidative stress in Islets of Langerhans. Pharm Biol, 54(10), 2061-2072. doi:10.3109/13880209.2016.1141222 3. Bhatt, N., Ghosh, R., Roy, S., Gao, Y., Armanios, M., Cheng, L., & Franco, S. (2016). Robust reprogramming of Ataxia-Telangiectasia patient and carrier erythroid cells to induced pluripotent stem cells. Stem Cell Res, 17(2), 296-305. doi:10.1016/j.scr.2016.08.006 4. Bhatt, N. M., Aon, M. A., Tocchetti, C. G., Shen, X., Dey, S., Ramirez-Correa, G., . . . Cortassa, S. (2015). Restoring redox balance enhances contractility in heart trabeculae from type 2 diabetic rats exposed to high glucose. Am J Physiol Heart Circ Physiol, 308(4), H291-302. doi:10.1152/ajpheart.00378.2014 5. Aon, M. A., Bhatt, N., & Cortassa, S. C. (2014). Mitochondrial and cellular mechanisms for managing lipid excess. Front Physiol, 5, 282. doi:10.3389/fphys.2014.00282 2 of 2
biochemistry, cancer, diabetic, experiments, GLP, good laboratory practice, peer-review
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Companies Worked For:
University of Maryland, Baltimore
Johns Hopkins University School of Medicine
Sun Pharma Advanced Research Company Limited Gujarat
The Maharaja Sayajirao University of Baroda
Sikkim Manipal University Distance Education
Job Titles Held:
Doctor of Philosophy Masters of Business Administration : Health Care Services Masters of Science : Biochemistry Bachelors of Science : Botany, Zoology, Chemistry
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