Highly motivated, interactive and flexible Bioinformatics Analyst with five years of NGS
and academic laboratory experience related to genomic projects. Strong technical
backgrounds and hands-on skills in molecular & cell biology, computational biology and
Next Generation Sequencing data collection, processing, mining and analysis.
Bioinformatics Next Generation Sequencing
Computational Biology Genomic data analysis (RNA-seq, WES, ChIP-seq
Statistical methods & ATAC-seq)
Linux/Unix environment Data processing, mining, analysis & management
High performance computing R/Bioconductor, Python, Java, and SQL
Network-based methods (WGCNA & other methods)
NGS Lab Manager & Business DevelopmentJun 2015 to Jul 2016 Quick Biology, Inc － Pasadena, CA
Work as an important member of co-founding team of Quick Biology, Inc, major contributor in establishing key NGS service pipelines for serving academic, medical and biotechnology customers.
Maintain and develop analysis pipelines for active customers' NGS genomic data (RNA-seq, WES, WGS, ChIP-seq, and ATAC-seq), regular back-up & testing of NGS server and analysis scripts.
Perform data analysis for whole genome, exome, and transcriptome related analysis variant detection, structural variant, transcriptome, and alternative splicing).
Coordinate and perform NGS data management for current customers: arrange, monitor the acquisition & delivery of sequencing data and final results to customer within schedule.
Manage daily operations of NGS lab including project management, personnel tasks assignment, budgeting, and managing lab inventory.
Provide training and guidance to new or current personnel in designing, conducting experiments, and bioinformatics analysis for NGS applications.
Implement and direct effective marketing & business plans to promote service to potential NGS customers in the market, resulted in a total of $350,000 orders in 5 months.
Manage customer accounts, invoicing, and maintain good customer relationships.
Provide technical service to customers in designing, troubleshooting, and verifying appropriate alternative analysis for NGS experiments.
Bioinformatics AnalystJan 2014 to Jun 2015 UCLA － Los Angeles, CA
Applied bioinformatics, molecular biology and large-scale data analysis to support various Next Generation Sequencing (NGS) research projects.
Processed, quality-controlled and analyzed NGS data for alignment, transcriptomic gene expression, canonical pathways using Python, Java and R, plus other publicly-available computational methodologies.
Performed statistical and bioinformatics analysis of large biological Next Generation Sequencing (NGS) data.
Conducted biological data mining and proposed testable hypothesis based on gene expression data to support research activities.
Managed and maintained sequencing data, records, reports, and files according to protocols.
Prepared summaries, presentations, manuscript sections and figures for the visualization and publication of genomic, metabolic and epigenomic data.
Participated and contributed in weekly group meetings on experimental design, activity planning, and troubleshooting.
Research ScientistJan 2010 to Jan 2014 Department of Veterans Affairs ? － Los Angeles, CA
Assisted principle investigator to design, plan and execute experiments for various cancer research projects.
Collected, generated and analyzed data and communicated research findings.
Selected and developed appropriate methods for experiments and reached project goals within schedule.
Wrote manuscripts for publication and participated in research grant funding applications.
Elucidated potential disease pathways and identified cancer-specific biomarkers incorporating transcriptomic data with IPA analysis.
Implemented alignment, variant calling, gene expression, alternative splicing and fusion genes analysis from RNA-seq data using online and commercial resources and programs.
Master of Science, Computer Science2017Georgia Institute of TechnologyComputer Science
Master of Science, Biochemistry2010California State University － Long BeachBiochemistry
Bachelor of Science, Biochemistry & Molecular Biology2006University of California － DavisBiochemistry & Molecular Biology
Liao, W., & Sharma, S. (2016). Modulation of B-cell receptor and microenvironment signaling by a guanine exchange factor in B-cell malignancies. Cancer Biology and Medicine, 13(2), . doi:10.20892/j.issn.2095-3941.2016.0026 Liao, W., Jordaan, G., Nham, P., Phan, R. T., Pelegrini, M., & Sharma, S. (2015). Gene expression and splicing alterations analyzed by high throughput RNA sequencing of chronic lymphocytic leukemia specimens. BMC Cancer, 15(1), . doi:10.1186/s12885-015-1708-9 Liao, W., Jordaan, G., Coriaty, N., & Sharma, S. (2014). Amplification of B cell receptor-Erk signaling by Rasgrf- 1 overexpression in chronic lymphocytic leukemia. Leukemia & Lymphoma, 55(12), 2907-2916. doi:10.3109/10428194.2014.898759 Jordaan, G., Liao, W., & Sharma, S. (2014). Identification of Histone epigenetic modifications with Chromatin Immunoprecipitation PCR array in chronic lymphocytic leukemia specimens. Journal of Cancer Science & Therapy, 06(09), .doi:10.4172/1948-5956.1000290 Liao, W., Jordaan, G., Srivastava, M., Dubinett, S., Sharma, S., & Sharma, S. (2013). Effect of epigenetic histone modifications on E-cadherin splicing and expression in lung cancer. American journal of cancer research, 3(4), 374-89. Jordaan, G., Liao, W., & Sharma, S. (2013). E- cadherin gene re-expression in chronic lymphocytic leukemia cells by HDAC inhibitors. BMC Cancer, 13(1), . doi:10.1186/1471-2407-13-88 Sharma, S., Liao, W., Zhou, X., Wong, D. T. W., & Lichtenstein, A. (2011). Exon 11 skipping of E-Cadherin RNA Downregulates its expression in head and neck cancer cells. Molecular Cancer Therapeutics, 10(9), 1751-1759. doi:10.1158/1535-7163.mct-11-0248