Structured Laboratory Technician offering background in collecting, processing and analyzing various specimens. Familiar with basic medical terminology, laboratory protocols and testing procedures. Expertise in in vitro and in vivo experiments involving mice. Recognized for performing laboratory tests and procedures with attention to detail, optimum quality and expedience.
- Molecular work: PCR, RT-qPCR, ELISA, western blot
- Cell culture of RAW and osteocyte-like MLO-Y4 cells
- Mice tissue sample collection and dissection
- en face analysis
- Histological sectioning and staining
- Light microscope and Confocal microscope Analysis
- microCT analysis
- Specimen and Quality Control Analysis
- Computer skills: MS Suite Proficiency (PowerPoint, Excel, Word), Image J, EndNote, GraphPad Prism 9
Advisor: Dr. No-Hee Park
- Thesis: Topical application of Porphyromonas gingivalis into the gingival pocket in mice leads to chronic-active infection, periodontitis and systemic inflammation
- 4.0 GPA
- Principal's List 2012, 2013, 2014
Charlotte, NC, 07/2022 - Current
- Continuing to perform in vitro and in vivo experiments with mice (i.e., inducing periodontitis on mice by placing silk ligatures around second maxillary molars.)
- Keeping research animals in good condition by upholding feeding and care schedules.
- Analyzing test data and presented findings using charts and graphs.
- Orienting and training new laboratory students and assistants in techniques.
- Executing new projects based on outlined research goals and project specifications.
Lexington, KY, 08/2020 - 07/2022
- Inoculated mice gingival pockets with highly concentrated Porphyromonas gingivalis (Pg).
- Performed en face analysis of mice artery and quantified lipids in the artery with Oil Red O staining and Sudan IV staining.
- Performed immunohistochemistry on paraffin-embedded maxillae and mice heart cyrosections.
- Performed PCR from mice gingival samples and RT-PCR for determining the expression of pro-inflammatory cytokines from gingival and artery samples.
- Performed In Situ Hybridization and localized Porphyromonas gingivalis RNA sequences.
- Performed microCT analysis on mice maxillae samples.
Warren, NJ, 10/2019 - 08/2020
- Performed in vitro experiments with mammalian cells.
- Performed animal experiments with mice i.e., general anesthesia, collection of whole blood, extraction of tissues, induction of periodontitis by placing silk ligatures around mice molars.
- Quickly learned new skills (i.e., PCR, RT-qPCR, immunohistochemistry, ELISA, mice heart cyrosectioning, en face analysis) and applied them to daily tasks, improving efficiency and productivity.
- Kept laboratory supplies prepared by inventorying stock, placing orders and verifying receipt.
- Cleaned and sterilized tools and equipment to maximize purity of specimens.
Publications:
- Jinsook Suh, Jessica Claire, Sung Hee Lee, Reuben Claire and No-Hee Park: Hyperlipidemia is necessary for the initiation and progression of atherosclerosis by severe periodontitis in mice. Molecular Medicine Reports, Aug;26(2):273-281. doi: 10.3892/mmr.2022.12789. Epub 2022 Jul 7.
- Sharon Claire , Yasuhiko Bando, Chungyu Chang, Jeonga Kwon, Berta Taverdi, Doohyun Claire, Sung Hee Lee, Hung Ton-That, Reuben Claire, Peter L. Nara, and No-Hee Park: Topical application of Porphyromonas gingivalis into the gingival pocket in mice leads to chronic‑active infection, periodontitis and systemic inflammation. International J of Molecular Medicine. June 15, 2022 https://doi.org/10.3892/ijmm.2022.5159
- Sharon Claire , Sung Hee Lee, Chungyu Chang, Hung Ton-That, Bo Yu, Reuben Claire, and No-Hee Park: Periodontitis, atherosclerosis and accumulation of Alzheimer's disease markers in the brain by topical Porphyromonas gingivalis into gingival sulcus in mice. In Preparation.
Scientific Presentations (Presented as a delegate for Dr. No-Hee Park):
- 2022 IADR Meeting (Virtual presentation)
“Hyperlipidemia is necessary for the initiation and progression of atherosclerosis by severe periodontitis in mice.”
- 2022 AADOCR/CADR Meeting (Virtual presentation)
“Systemic disorders induced by Porphyromonas gingivalis inoculation into gingival pocket.”
- 2021 IADR/AADR/CADR Poster Presentation
“Periodontitis/Systemic inflammation following topical Porphyromonas gingivalis into gingival pocket.”
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School Attended
- University of California, Los Angeles
- University of California, Los Angeles
- Korea KENT Foreign School
Job Titles Held:
- Laboratory Assistant
- Student Laboratory Assistant
- Laboratory Technician
Degrees
- Master of Science
- Bachelor of Science
- High School Diploma
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