PhD in Life Science( Molecular Oncology and Medical Biotechnology) with 11 years of postdoctoral research experience
8 years research experience in Human Solid Tumor and Hematological Malignancies
More than 15 years of research experience in Cell and Molecular Biology field
4 years of postdoctoral research experience in HIV research field
10 years experience in designing, analyzing research data, writing articles for publication and presentation of data in meetings and conference
Extensive experience in Human genetics and functional genomics, bioinformatics
Experience in Managing research groups and projects.
Extensive experience in planning and executing new research projects, experiment, preparing protocol, statistical analysis of data, interpretation of results and preparation of report
10 years of experience in collaborative research skills
Excellent oral and written communication skills
Nucleic Acid and protein Isolation from Human solid tumor
Southern and Western Blotting
Nucleic Acid Pull down
Site Directed Mutagenesis
Gel Shift Assay (EMSA)
GST-pull down assay
Mammalian Cell culture
Isolation of primary cells from tissue and culture
Generation of stable cell lines
Mouse Tumor Model
Cell Proliferation assay
CD4T cell isolation and culture
Cell surface marker staining
Genomic software (CLC, Primer3, Genomatix)
Statistical Data analysis
PhD (Molecular Oncology): Life Science2004Utkal UniversityBhubaneswar, Odisha, India
Thesis: Molecular Markers of solid tumors and leukemia With Special Reference to p53 tumor suppressor gene
Collected Breast cancer, oral cancer, cervical cancer tissue from cancer center
Isolated DNA, RNA, tissue extract and nuclear extract from cancer tissue
Detected p53 gene mutation in solid tumor tissue by PCR-SSCP
Detected gene alteration in p73 gene by methylation assay
Conducted rearrangement analysis of p53 gene in cervical cancer by southern blotting
Conducted polymorphism analysis in HPV 16 and 18 types in oral and cervical cancer
Investigated regulation of apoptosis by poly ADP polymerase
Performed experiments on protein expression and accumulation in Human cancer tissue by western blotting and immunohistochemistry
Investigated binding of transcription factors (NF1, YY1, SP1, CAAT, PAX) in different grades of tumor tissue using Gel shift assay (EMSA)
Master of Science: Zoology and Biochemistry1997Berhampur UniversityBerhampur, Odisha, India
Bachelor of Science: Zoology1993Berhampur UniversityBerhampur, Odisha, India
Research Scientist in Virology11/2014 to CurrentGeorge Washington UniversityWashington, DC
Investigated a project focused on " effect of cocaine on HIV replication with reference to epigenetic modification in HIV promoter''
Designed a project focused on "Inhibition of HIV replication by DNA PK inhibitor"
Written and published a first author article in Virology journal
Established viral reporter cell lines and produced lentivirus
Isolated CD4T cells from Human Blood and performed surface marker staining for flow cytometry analysis
Trained 2 postdoctoral fellow, 3 summer trainee and one lab technician
Research Associate in Immunology09/2011 to 11/2014St Jude Children's Research HospitalMemphis, TN
Investigated a project entitled “ Regulation of Myc induced nuclear antigen (MINA) by TGF-b and smad3 in naive CD4T cells of Th2 biased mice”
Developed transgenic mice (Villin-Mina) by recombinant DNA technology
Isolated and cultured naive CD4T cells from spleen and lymphnode of mouse
Constructed various clones for conditional reportermouse project using cre-loxp technology
Used CLC software for sequence analysis of cloned plasmid
Used Graph pad and Image J software for statistical and protein expression analysis
Perfomed Real Time PCR for RNA quantitation
Performed transcription factor binding site analysis in gene promoter using UCSF and Genomatix website
Constructed GST-Mina clones to express and purify proteins in BL21 cells
Presented weekly research reports to supervisors and peer groups
Postdoctoral Research Scientist in Oncology06/2009 to 09/2011Georgetown UniversityWashington, DC
Investigated and completed a project entitled “ Regulation of Mitochondrial Citrate Transporter by Mutant p53”In Breast Cancer
Investigated "Interaction between mutant p53 and FOXO1 protein in breast cancer"
Isolated and cultured Microvascular endothelial cells from rat lungs for various cellular assay
Injected breast cancer cell lines to mice for tumor developement
Constructed various mutant clones by site directed mutagenesis
Analysed transcription factor binding sites in gene promoter by Genomatix software
Used Graph pad prism software for statistical analysis
Investigated DNA-Protein interaction by Nucleic Acid Pull down and chromatin immunoprecipitation assay (CHIP).
Published three research papers
Supervised one postdoc and one technician
Visiting Fellow (Molecular Virology)12/2005 to 05/2009National Institute of HealthBethesda, MD
Completed a training project focused on "Integration of Tat into HIV virion that enhances viral replication"
Transfected plasmid to generate HIV virus
Performed western blotting, luciferase assay and ELISA
Maintain mammalian cell lines
Performed in vitro transcription and translation assay
Concentrated HIV virion by sucrose gradient
Identified efficacious siRNAs for multiple target genes for in vivo studies
Sahu G, Farley K, EL-Hage N, Aiamkitsumrit B, Fassnacht R, Kashanchi F, Ochem A, Simon GL, Karn J, Hauser KF, Tyagi M (2015), Cocaine Promotes both initiation and elongation phase of HIV-1 transcription by activating NF-kB and MSK1 and inducing selective epigenetic modifications at HIV-1 LTR, Virology, 483:185-202. (Impact factor : 3.278)
Kolukula VK, Sahu G, Wellstein A, Preet A, lacobazzi V, D'Orazi G, Albanese C, Palmieri F, Avantaggiati ML (2014), SLC25A1, or CIC, is a novel transcriptional target of mutant p53 and a negative tumor prognostic marker, ONCOTARGET, 5(5):1212-25.(Impact factor: 6.63)
Kirilyuk A, Simoji M, Catania J, Sahu G, pattabiraman N, Albanese C, Giordano A, Mocchetti I,Toretsky JA, Uversky VN, Avantaggiati ML (2012), An intrinsically disordered region of acetyltransferase p300 with similarity to prion like domains plays a role in aggregation, PLOS ONE, 7(11):e48243. (Impact factor: 4.1)
Kandasamy K, Sahu G, Parthasarathi K (2012), Real time imaging reveals endothelium mediated leukocyte retention in LPS treated lung microvessel, Microvascular Research, 83(3):323-31. (Impact factor: 2.929)
Sahu G, Jena RK (2011), Clinical Significance of p53 and Bcl-2 in Acute myeloid Leukemia Patients of Eastern India, Hematology Report, Oct 19; 3(3):e28.
Perez RE, Knights CD, Sahu G, Catania J, Kolukula VK, Stoler D, Graessmann A, Ogryzko V, Pishvaian M, Albanese C, Avantaggiati ML (2010), Restoration of DNA binding and growth suppressive activity of mutant forms of p53 via a PCAF mediated acetylation pathway, Journal of cellular Physiology, 225(2):394-405. (Impact factor: 4.218)
Sahu G, Wang DF, Chen Claudia B, Victor B Zhurkin, Rodney E Harrington, Ettore Appella, Gorden L Hager, Akhilesh K Nagaich (2010), p53 Binding to Nucleosomal DNA Depends on the Rotational positioning of DNA response element, Journal of Biological Chemistry (JBC), 285(2): 1321-1332. (Impact factor: 4.77)
Sahu G, Das BR (2005), Alteration of p73 in denovo pediatric Acute Lymphoblastic Leukemia, Biochemical and Biophysical Research Communication, 327(3): 750-5.(Impact factor: 2.4)
Sahu G, Jena RK (2005). Significance of intracellular Arsenic trioxide for Therapeutic response in Acute promylocytic leukemia, American Journal of Hematology, 78 (2): 113-6. (Impact factor: 4.138)
Sahu G, Nayak B, Patnaik S, Parija T, Das BR (2002). Rearrangement of p53 gene with overexpressed p53 protein in primary cervical cancer. Oncology Report, 9: 433-437.(Impact factor: 2.297)
Sahu G, Das BR (2002).Prognostic significanceof p53 and Bcl-2 in lymphoblastic leukemia. Oncology Report, 9: 1391-1398. (Impact factor: 2.297)
Therapeutic targeting of the mitochondrial citrate career, CIC, in tumors harboring p53 mutations: Rationales and Challenges, 16th International p53 workshop, (2014), June (15-19). Stockholm, Sweden.
Dermal Mast Cells and Fibrosis: Correlation to Keloid Pathology, Society for Investigative Dermatology, Annual Meeting,(2014), May (7-10). Albuquerque, New Mexico, USA.
Real-time fluorescence microscopy reveals ICAM-1-dependent leukocyte margination in micro vessels of LPS- treated lungs. Experimental Biology Conference,(2011), April (9-13).Washington, DC [FASEB Journal: 2011; 25:lb639].
Corruption of the activity of mitochondria citrate transporter, CIC, by mutant forms of p53.American Association for Cancer Research, 101ST Annual Meeting 2010, April 17-21, (2010), and Washington DC. Cancer Research: 2010; Volume 70, Issue 8, Supplement 1.
p53 Binding to Nucleosome at the Dyad is Dependent on the Rotational Positioning of Response Element DNA and is accompanied by Partial Loss of Histone H3.NIH Research Festival (2008) October 14-17, Bethesda, Maryland.
Plasmid Vectors for in vitro and in vivo delivery of siRNAs. 23rd Annual Convention of the Indian Association for Cancer Research, (2004).
Studies on tissue expression pattern of PRMT family members using Anti-PRMT Polyclonal Antibodies. 22nd Annual Convention of the Indian Association for Cancer Research, (2003).
Molecular Markers involved in Breast Cancer Patients of Eastern India with special reference to p73 tumor suppressor gene. 22nd Annual Convention of the Indian Association for Cancer Research, (2003).
Involvement of p53 and Bcl-2 gene in acute leukemia with reference to overall survival analysis- A study of 100 cases. 21st Annual Convention of the Indian Association for Cancer Research, (2002).
Involvement of Molecular Prognostic Markers in Acute Leukemia. 42nd Annual National Conference of Indian Society of Hematology and Transfusion Medicine, (2001).
Cytogenetic as prognosticator in Acute Lymphoblastic Leukemia.42ndAnnual National Conference of Indian Society of Hematology and Transfusion Medicine, (2001).
Awards and Fellowship
Award from American Physiological Society as a featured speaker in Experimental Biology Conference, Washington, DC (2011)
ORISE Fellow Award, Oak Ridge Institute for Science and Education, Oakridge, TN (2007)
NIH Visiting Fellow Award, Bethesda, MD (2005)
Senior Research Fellow, Council for Scientific and Industrial Research, India (2000-2002)
Junior Research Fellow, Council for Scientific and Industrial Research, India (1998-2000)
Qualified Graduate Aptitude Test in Engineering (GATE-97), India
American Association for Advancement of Science (AAAS)