Safety-conscious Clean Room Technician with two-years background maintaining clean work areas, conducting preventive maintenance and operating filling machine. Comfortable handling and disposing of hazardous waste. Skilled Microbiology Technician and Quality Technician with experience in the microbiologist field.
Skills
Protein Purification techniques- Thin-Layer Chromatography, Gel-Filtration Chromatography, Ion-Exchange Chromatography, High Performance Liquid Chromatography, Affinity Chromatography, SDS-PAGE
DNA extraction techniques- Polymer Chain Reaction, Agarose Gel Electrophoresis
Protein Characterization- FTIR, NMR, UV-Vis Spectroscopy
Karl Fischer Titration
3D Visualization of macromolecules using PyMOL
Origin Lab, LIMS, MS Office Suite
Work History
Quality Control Technician, 09/2021 - Current A & E Television Networks – Los Angeles, CA,
Determined root cause of deviations and non-conforming results and implemented appropriate corrective and preventive actions throughout product development process.
Collect viable and non-viable samples within controlled environments.
Collect viable and non-viable samples for compressed gases.
Collect personnel glove and gown samples.
Responsible for supporting Quality Control testing for release of clinical products.
Participate in supporting document management, projects, CAPAs and investigations in accordance with the organizations policies, procedures and state, federal and local laws and compliance with current Good Manufacturing Practices (cGMP), USP, EP, JP and other regulatory requirements at all times.
Microbiology Laboratory Technician, 08/2020 - 08/2021 Beyond Meat – King Of Prussia, PA,
Aseptically performs microbiology sampling, plating, and testing of samples daily.
Always adhere to safety regulations by following safety standards and protocols regarding safe handling materials and wearing proper protective equipment.
Conducts microbiological qualitative or quantitative analyses by meticulously following all SOP’s and laboratory methods.
Daily check and record temperature of all refrigerators, freezers, and water baths in Microbiology department.
Maintain proper record keeping for media preparation, autoclaves, pH.
Kept laboratory and work areas clean and organized.
Recorded data on computer using LIMS by following standard operating methods.
Preparation of 10x diluent solution using stir plate.
Preparation of media and test solutions for detection of Nitrifying bacteria.
Colony counting using automatic colony counter flash and go.
Discard of biohazard waste by using puncture proof biohazard waste containers.
Preparation of Media using AES Chemunex APS ONE Masterclave 528.
Quality control of culture media by checking parameters such as growth supporting characteristics and batch contamination.
Determination of Escherichia coli and Coliforms using CHROMagar, determination of Acid-Producing Bacteria using BTI-APB Media, determination of Denitrifying Bacteria using nitrile broth tubes.
Enumeration of Sulfate Reducing Bacteria, Clostridium, Bacterial spores, Pseudomonad's, Pigmented Bacteria, Mucoid Bacteria, and Molds and Yeasts.
Kept laboratory equipment clean and in good working order for optimal functionality and longevity.
Recorded, reported and posted test results using LIMS.
Maintain operating policies and procedures of company as well as requirements specified by FDA, OSHA and other governmental agencies.
Making of sterile pre-filled syringes including Normal Saline I.V. Flush, Heparin Lock Flush (1Unit/mL, 10 units/mL and 100 units/mL) by using automated filling machine.
Complied with cGMP activities.
Read and understood all SOPs to related area.
Participated in cleaning and sanitization cleanroom complex after production is complete.
Participated in Environmental monitoring activities.
Disinfected and sterilized filling machine parts to destroy microorganisms.
Prepared equipment by performing sterile cleaning and conducting preventive maintenance checks.
Documented [perform activities in batch records.
Used different tools to make minor mechanical adjustments to equipment.
Maintained clean, neat, and organized work areas to prevent safety hazards, reducing workplace incidents.
Performed cleaning techniques by thoroughly vacuuming clean room area, removing trash and bio-hazard materials and mopping walls, ceilings and floors.
Used allen wrenches to make minor mechanical adjustments to equipment.
Education
Bachelor of Science: Biochemistry, Expected in 05/2020 - University of Illinois At Chicago - Chicago, IL GPA:
Research Assistant Experience
Worked as volunteer research assistant in University laboratory.
Analysed organic and inorganic compounds from water using HPLC and Gas chromatography.
Characterized products with 1 H NMR, UV-Vis, and IR spectroscopy.
Operated wet methods and instrumentation analysis.
Bachelor of Science: Microbiology, Expected in 12/2015 - Sardar Claire University - Gujarat, India, GPA:
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