Efficient and productive Cytogenetics technologist well-rounded in the different types of tests execution and analysis, including FISH tests, chromosome and MicroArray and with extensive knowledge of genetics, molecular biology, medicine and biochemistry.
•ASCP Board of Certification for technologist in cytogenetics, CG(ASCP).
•License in cytogenetic technologist: State of Florida, License No.
•License in clinical cytogeneticist scientist: State of California, License No.
•Routinely performed the different kinds of specimens; including amniotic fluid, peripheral blood, bone marrow and paraffin slides for fluorescence in site hybridization (FISH) tests.
•Properly analyzed FISH slides using a computer-assisted microscope imaging system; counted signals and recorded the results with proper ISCN nomenclature.
•As a team leader of the FISH section, highly motivated to improve the quality of FISH signals and contributed to improve FISH processes for paraffin slides. Additionally, oversaw and trained other techs and trainees.
•Prepared specimens using standardized cytogenetic culture, harvest, slides-making procedures, and using routine G-band chromosome staining for the different types of samples including amniotic fluid, product of concept, CVS, peripheral blood, bone marrow and solid tumors.
•Screened and imaged cytogenetic specimens using a computer-assisted microscope imaging system, counted chromosomes, made karyotype and described the abnormalities of chromosomes with proper ISCN nomenclature.
•Performed Array-Based CGH for Genomic DNA analysis, including DNA extraction, digestion, labeling and preparation of labeled genomic DNA for hybridization.
•Troubleshot instruments and/or procedure failures.
•Performed quality control and validation/implementation for new tests/methodologies.
•Maintained accurate accession records of specimens.
•Monitored the daily workflow and sent results within TAT goals in FISH section.
Closely followed and learned knowledge and techniques in cytogenetics laboratory testing.
•Protein extraction and analysis, Western blot for proteins using chemiluminiscent reagents.
•Isolation of DNA and RNA, RT-PCR, Real-Time RT-PCR.
•Performed immunuhistochemistry and immunofluorescence double staining.
•Isolation and maintenance of smooth muscle cells from pulmonary artery.
•Embryonic lung explant culture.
•Captured histochemical images from fluorescence microscope.
•Animal handling and maintenance.
•Surgery, dissection, tissue collection (including fresh/fixed lung and embryonic lung)
•Systemic and local in vivo drug dosing and administration via IV (implantation of catheter into the vein), IP (intraperitoneal), SC (subcutaneous).
•Perfusion (using paraformaldehyde fixative).
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