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Associate Research Scientist Resume Example

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ASSOCIATE RESEARCH SCIENTIST
Links
  • linkedin.com/in/gaya-yadav-56085721
  • https://www.researchgate.net/profile/Gaya_Yadav
  • https://scholar.google.com/citations?hl=en&user=GJ2E2fEAAAAJ
Professional Summary

Analytical Research Specialist with experience in experimental research and data analysis. Detail-oriented and methodical professional successful in developing in-depth research projects. Reliable team player with excellent communication and problem-solving skills.

Skills
  • Documentation expertise
  • Enzyme kinetics/affinity and mechanism of inhibition results analysis
  • Laboratory Management.
  • Electron microscopes: Titan Krios, Glacios, Tecnai F20, ZEOL2200FS etc.
  • Single particle Analysis (cryo-EM) using different packages such as RELION, cisTEM, cryoSPARC, etc
  • Writing Research grants
  • Good Laboratory Practices (GLP)
  • Organization and Time management
  • Written Communication
  • Recombinant protein expression in E. coli, insect cell and mammalian systems,
  • Protein purification by FPLC (AKTA/NGC purification system, affinity/IEX/size exclusion chromatography), western blotting, ELISA, immuno-precipitation, etc.
  • Surface plasmon resonance (SPR), fluorescence polarization (FP), isothermal titration calorimetry (ITC), differential scanning flourimetry (DSF), dynamic light scattering (DLS), circular dichroism (CD), fluorescence, etc.
  • Cryo-EM, X-ray crystallography
  • Microsoft office suite, Prism, ImageJ, Pymol, Chimera, Autodock4, Glide, Relion, Coot, etc.
Work History
Associate Research Scientist01/2020 to Current
Company Name – City, State
  • Performed day-to-day operation of transmission electron microscopes (cryo-TEMs), prepared cryo-EM samples and collected data.
  • Process cryo-EM data to determine structures of protein complexes in near native states.
  • Conducted protein purification from different over-expression systems such as bacterial, Insect cells (baculoviral), yeast (Pichia and cerevisiae), and mammalian over-expression systems.
  • Handled different kind of cells cultures (bacterial, insect cells, yeast cells and mammalian cells).
  • Performed biochemical and biophysical assays to characterize proteins functions and conformations.
Biological Scientist III02/2019 to 01/2020
Company Name – City, State
  • Wrote research papers, reports, reviews and summaries regarding role of chromogranin ion channels in regulation of pH in secretory granules.
  • Conducted protein purification from different over-expression systems such as bacterial, Insect cells (baculoviral), yeast (Pichia and cerevisiae), and mammalian over-expression systems.
  • Handled different kind of cells cultures (bacterial, insect cells, yeast cells and mammalian cells).
  • Performed biochemical and biophysical assays to characterize proteins functions and conformations.
  • Used Microsoft office suite, Prism, ImageJ, Pymol, Chimera, Autodock4, Glide, Relion, Coot, cryoSPARC, cisTEM, ORIGIN, ChemDraw, EPU, Leginon, etc.
Postdoctoral Associate 02/2016 to 01/2019
Company Name – City, State
  • Wrote research papers, reports, reviews and summaries regarding role of chromogranin ion channels in regulation of pH in secretory granules.
  • Performed cryo-EM data collections using transmission electron microscopes (cryo-TEMs).
  • Process cryo-EM data to determine structures of protein complexes in near native states.
  • Conducted protein purification from different over-expression systems such as bacterial, Insect cells (baculovirus), yeast (Pichia and cerevisiae), and mammalian over-expression systems.
  • Handled different kind of cells cultures (bacterial, insect cells, yeast cells and mammalian cells).
  • Mentored and led many rotation/intern and graduate students in their research and experiment design.
Postdoctoral Researcher08/2011 to 01/2016
Company Name – City, State
  • Wrote research papers, reports, reviews and summaries regarding role of chromogranin ion channels in the regulation of pH in the secretory granules.
  • Process cryo-EM data to determine the structures of protein complexes in near native states.
  • Handled different kind of cells cultures (bacterial, insect cells, yeast cells and mammalian cells).
  • Performed biochemical and biophysical assays to characterize proteins functions and conformations.
Education
Ph.D.: Biochemistry And Biophysics08/2011Jawaharlal Nehru University- City

1. Characterized role of exogenously added mycobacterium phosphoserine phosphatase (MtSerB2) induces microtubule rearrangements in THP-1 cells. (https://doi.org/10.1371/journal.pone.0115409).

2. Determined X-ray structure of HIV-1 Nef : an important accessory protein contributing to multiple distinct functions of the virus, such as immune evasion, virion infectivity, and support for viral replication and survival (https://doi.org/10.1371/journal.pone.0026629).

Master of Science: Chemistry07/2003University Of Lucknow- City
Accomplishments
  • Used Microsoft Excel to develop inventory tracking spreadsheets.
  • Supervised team of undergraduate students members.
  • Streamlined the automatic data collection pipeline for the cryo-EM image acquisition.
  • Academic Editor: Journal of Cellular Signaling (https://www.scientificarchives.com/editor/gaya-prasad-yadav).
  • Academic Editor: Trends in Clinical Microbiology.
  • Editorial board member: Journal of Pharmaceutics and Drug Research. (http://www.scitcentral.com/editorboard.php?journal=17)
  • Associate Editor: Endocrinology & Metabolism International Journal (https://medcraveonline.com/EMIJ/editorial-board).
  • Reviewer: multiple journals.
Additional Information

SELECT PUBLICATIONS

1. Mohammed E. Sayed, Ao Cheng, Gaya P. Yadav, Andrew T. Ludlow, Jerry W. Shay, Woodring E. Wright, and Qiu-Xing Jiang; Catalysis-dependent inactivation of human telomerase and its reactivation by intracellular telomerase-activating factors (iTAFs). J. Biol. Chem. June 11, 2019.

2. Gaya P. Yadav, Hui Zheng, Qing Yang, Lauren G Douma, Linda B Bloom, and Qiu-Xing Jiang; Secretory granule protein chromogranin B (CHGB) forms an anion channel in a membrane. Life Science Alliance, Sept 2018, 1(5) e201800139; DOI:10.26508/lsa.201800139.

3. Yadav GP, Current Dilemma on Granin Proteins: Proteins involved in various cellular functions without known mechanisms. Cell Cellular Life Science J (2017), 2(2):000115.

4. Liu H, Liang C, Kollipara RK, Matsui M, Ke X, Jeong BC, Wang Z, Yoo KS, Yadav GP, Kinch LN, Grishin NV, Nam Y, Corey DR, Kittler R, Liu Q. Mol Cell. (2016) 4; 63(3):420-32. DOI: 10.1016/j.molcel.2016.06.014. Epub 2016 Jul 14.

5. Yadav GP, Shree S, Maurya R, Rai N, Singh DK, et al. Characterization of M. tuberculosis SerB2, an Essential HAD-Family Phosphatase, Reveals Novel Properties. (2014) PLoS ONE 9(12): e115409.

6. Singh P*, Yadav GP*, Gupta S, Tripathi AK, Ramachandran R, Tripathi RK. A novel dimer-tetramer transition captured by the crystal structure of the HIV-1 Nef. PLoS One. 2011; 6(11): e26629. (*Equal author).

Book Chapters

1. Gaya P. Yadav and Qiu-Xing Jiang; Recosntituted Membrane Systems for Assaying Membrane Proteins in Controlled Lipid Environments. (Book Chapter in “New Techniques for Studying Biomembranes”,Taylor & Francis Group, April 7, 2020).

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Resume Overview

School Attended

  • Jawaharlal Nehru University
  • University Of Lucknow

Job Titles Held:

  • Associate Research Scientist
  • Biological Scientist III
  • Postdoctoral Associate
  • Postdoctoral Researcher

Degrees

  • Ph.D. : Biochemistry And Biophysics 08/2011
    Master of Science : Chemistry 07/2003

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