Microsoft Word, Excel, PowerPoint, Adobe Acrobat, Adobe Photoshop, Infotrieve Electronic Laboratory Notebook (ELN), Filemaker Pro, GraphPad Prism, Igor Pro IVIS and FACS Express
Charles River Short Course, Boston, MA, 2002.
Gas Anesthesia Small Rodent Workshop, UCLA, CA, 2002.
Sterile Rodent Surgery: A Hands on Workshop, Amgen, CA, 2001.
Regulated Species: A Hands-On Workshop, Amgen, CA, 2000.
Staff Research Associate III January 2010 to CurrentUniversity of California Los Angeles UCLA MIMG － Los Angeles, CA.
Breeding core manager; breeding and maintenance of NSG (immunodeficient mice) colony.
Time matings of NSG mice.
Assist with small rodent surgeries and Xenogen imaging.
Perform tail vein injections and intratibial injections in adult mice.
Facial vein and intrahepatic injections in neonate mice.
CIRM project: Sickle cell disease gene therapy in rodent model, CD34+ human bone marrow transduced with lentiviral vector to correct genetic disease.
Senior Research Associate, Research Associate II January 2003 to January 2010City of Hope (COH), Beckman Research Institute CITI － Duarte, CA.
Murine model manager; responsible for organizing, performing and analyzing animal experiments.
Supervised Research Associate II and managed workflow.
Proctor and train research associates, research fellows, graduate students and summer students in experimental in-vivo procedures and equipment operation.
Responsible for training COH staff with the operation of the Xenogen IVIS system for the Small Animal Imaging Core Facility.
Write, revise IACUC protocols, amendments and standard operating procedures.
Long term tissue culture maintenance of suspension and adherent lines.
In vivo therapy of engineered IL-13zetakine T cells targeting glioma and medulloblastoma in the mouse model.
In vivo T cell proliferation assays; harvest blood, bone marrow, spleen and liver to determine T cell engraftment by FACS analysis Track bioluminescent human T cells in vivo with Xenogen IVIS system.
Inoculate human brain tumors and T cells in mice via stereotaxic device intracranially, including intraperitoneal, subcutaneous and intravenous injections in mice.
Propagation of Primary Brain Tumors subcutaneously in NSG immunodefecient mice.
Cryoanesthesia of murine neonates and intracranial tumor inoculations.
Drug optimization studies dosing and feed studies (ie.
Lapatinib, Tamoxifen and Dexamethasone) Gross Necropsy, post transcardial fixation, harvest tissue, fresh freeze tissue and/or paraffin embedding for histological staining and examination.
Inventory control of immunodefecient mouse colonies and equipment maintenance.
Animal Health Technician January 1998 to January 2003ALAT, and Lab Animal Caretaker, City of Hope, Beckman Research
Institute DCM Animal Resources Center ARC － Duarte, CA
Coordinated daily veterinary care activities at the ARC an AAALAC accredited facility.
Observed animal colonies for signs of illness, maintained thorough health and history records of animals.
Supervised and coordinated student internship program for the ARC.
Provided guidance and consultation to investigators in matters of anesthesia, euthanasia, and procedures.
Stocked, dispensed, administered and maintained records of medications and treatment.
Conduct veterinary diagnostic procedures, maintenance of preventive medicine programs including routine parasitology, maintenance of sentinel animal program, quality control testing, and submissions of animals, tumors and cell lines, and pathological specimens to contract diagnostic laboratories.
Coordinated shipping of domestic and international live laboratory animals and laboratory specimens.
Performed gross necropsies, pre and post surgical support, care and monitoring; and performed or assisted with surgical procedures.
Perform polyclonal antibody production in rabbits; blood collection of marginal ear vein.
Conduct artificial insemination in chickens for production of viable egg embryos.
Trained personnel, research associates, and interns in animal biomethodologies: intradermal, subcutaneous, intramuscular, intraperitoneal, intravenous injections and intracardiac puncture.
Writing of IACUC protocols and standard operating procedures.
Established the usage of isoflurane gas anesthesia in mice and rats.
Maintained and coordinated mouse breeding colonies.
Husbandry of rats, mice, xenopus laveis, white leghorn chickens and New Zealand rabbits.
Sanitation of cages, animal holding rooms and operation of cage wash.
Intern East Valley Emergency Pet Clinic － Diamond Bar, CA
June 2009 (60 hours) Assisted and restrained cats and dogs for euthanasia, catheter placements, intubations, and X-ray radiographs.
Blood collection of jugular and cephalic veins.
Demonstrated placement of intravenous catheters and IV pump.
Demonstrated placement of endotracheal intubation and surgically prepped dogs for general anesthesia.
Assist Veterinarian in surgery.
Independently ran CBC/Chem 17 test and electrolytes in IDEXX machine.
Independently ran FIV/FILV test and Parvo Elisa test.
Parenteral administration of drugs, SC, IM and IV.
Demonstrated euthanasia of dogs via cephalic vein.
Learned the assessment of emergency procedures in cats, dogs and exotic animals.
Volunteer, Equine Research Center, California Polytechnic University, Pomona, CA, June 2001 (80 hours) Study equine health, function exercise and physiology; high altitude acclimatize horses study locomotion and energetics.
Placement of electrocardiogram (ECG) leads on horses and continuous monitoring of ECG at rest and during exercise on treadmill.
Acclimated horses to trotting level and decline prior to data collection.
VO2 under moderate condition was measured (using open flow respirometry) during trotting and canter on a treadmill on low and high incline.
Analyzed serum samples detecting lactate levels in blood from mules acclimated at high altitude levels.
Bachelor of Science : Animal Science Physiology and Zoology, 2003California State Polytechnic University － Pomona, CAAnimal Science Physiology and Zoology
Registered Veterinary Technology Program, Alternate Route, 2002 CERTIFICATIONS/LICENSE Registered Veterinary Technician (RVT), CA, 2010 Registered Laboratory Animal Technologist (RLATG), AALAS 2009 Assistant Laboratory Animal Technician (ALAT), AALAS 2000 Mount San Antonio Community College － Walnut, CA
Christine E. Brown, Renate Starr, Catalina Martinez, Brenda Aguilar, Massimo D'Apuzzo, Ivan Todorov, Chu-Chih Shih, Behnam Badie, Michael Hudecek, Stanley R. Riddell, and Michael C. Jensen. Recognition and Killing of Brain Tumor Stem-like Initiating Cells by CD8+ Cytolytic T cells. Cancer Res 2009; 69: (23). December 1, 2009. Jelena Lazovic, Evette Ferkassian, Brenda Aguilar, Michael Jensen, Andrew Raubitschek, Russell E. Jacobs. Imaging immune response in vivo: Cytolytic action of genetically altered T-cells directed to glioblastoma multiforme. Clin Cancer Res. 2008 Jun 15;14(12):3832-9. Christine E. Brown, Reena P. Vishwanath, Brenda Aguilar, Renate Starr, Joseph Najbauer, Karen S. Aboody, and Michael C. Jensen. Tumor-Derived Chemokine MCP-1/CCL2 is Sufficient for Mediating Tumor Tropism of Adoptively Transferred T cells. J.Immunol. Sep; V.179(5):3332-41,2007. Pameijer CRJ, Naranjo A, Meechoovet B, Wagner JR, Aguilar B, Wright CL , Chang W-C , Brown CE and Jensen MC. Conversion of a tumor-binding peptide identified by phage display to a functional chimeric T cell antigen receptor. Cancer Gene Ther. Jan; 14(1): 91-7, 2007. Lufen Chang, Catalina Martinez, George McNamara, Brenda Aguilar, Julie R. Ostberg and Michael C. Jensen. Transgene Enforced Costimulation of CD4+ T cells leads to Enhanced and Sustained Anti-Tumor Effector Functioning". Cytotherapy-2007-0091. POSTER PRESENTATIONS/ABSTRACTS Posters: ASGT Meeting May 30-June 3, 2007 1. Reena P.Vishwanath, Christine E. Brown, Brenda Aguilar, Wen-Chung Chang, Michael C. Jensen Intracranial Glioma Tropism of Genetically Modified T Lymphocytes Following CNS Intraparenchymal Adoptive Transfer (Presenter) 2. Christine E. Brown, Renate Starr, Brenda Aguilar, Michael C. Jensen Targeted elimination of Stem-Like Glioma Progenitors with Genetically Engineered Cytolytic T Cells
Adobe Acrobat, Adobe Photoshop, oral, CA, consultation, SC, data collection, Elisa, Filemaker Pro, Image Processing, Imaging, Inoculate, ie, Inventory control, Excel, PowerPoint, 2000, Microsoft Word, optimization, organizing, personnel, Prism, protocols, quality control, Express, Research, shipping, Technician, workflow
Research Associate Organization (RAO) - Executive Committee Member, President, 2010 - present
Research Associate Organization (RAO) - Executive Committee Member - Secretary, 2007 - 2009
American Association of Laboratory Animal Science (AALAS), Member, 2000 - present