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senior research associate resume example with 10+ years of experience

Jessica Claire
  • , , 609 Johnson Ave., 49204, Tulsa, OK 100 Montgomery St. 10th Floor
  • H: (555) 432-1000
  • C:
  • resumesample@example.com
  • Date of Birth:
  • India:
  • :
  • single:
  • :
Professional Summary

Self-motivated and detail-oriented scientist with strong leadership skills and proven expertise in microbial fermentation and process development, molecular biology, and microbiology, Jessica offers 8 years industry experience which includes upstream high throughput strain screening in small scale fermentation and bench scale ferment's up to 10L . Experience producing target proteins for drug design . Experience working with heterologous enzymatic production in yeast and bacteria for biofuels and biochemicals, in anaerobic and aerobic conditions.

Skills
  • Process development and fermentation.
  • Mini and bench scale bioreactor validation.
  • High throughput strain screening and media optimization.
  • Protein purification: AKTA FPLC, his-tag column, biotin streptavidin column, TFF.
  • Molecular and Cellular Biology: ELISA, Primer designing, PCR, Real time PCR. Molecular gene Cloning
  • Outstanding leadership, presentation and communication skills written and verbal communication in English and Portuguese with intermediary of Spanish
Work History
PhD Graduate Assistant, 08/2019 - Current
Brown University Providence, RI,
  • Setting up bioreactors and starting fermentation bioprocess
  • Training operation of Bioflo15 to the team, data analysis, set up of the bioreactor.
  • Post run enzyme purification assay development.
  • Experience in batch, fed batch, chemo stats and semi continuous feed and bleed method of fermentation in mid-size bench top bioreactors 250mL, 1L to 10L, Applikon and Eppindorff
  • Lead the project on Identification, characterization of microbial consortia from mammalian herbivores for the production of volatile fatty acids and other valuable biochemicals for food industry and animal nutrition.
  • Design, set up and maintain bench scale stirred tank bioreactor, 1L to 5L, for anaerobic fermentation of lignocellulosic biomass and hydrogen gas.
  • Experience in process development in 1L-5L Applikon stirred tanks.
  • Protein purification: Anion exchange chromatography (FPLC AKTA), affinity chromatography (histag)
  • Responsible for improvement of production of biochemicals in anaerobic conditions.
  • Bacterial DNA and RNA isolation and Real time PCR using nonspecific Sybr green.
  • Detail knowledge in primer designing, using of lasergene, seqbuilder, sequence alignment, sequence analysis, designing of site directed mutagenesis.
  • Cell culture: bacterial and archean cells
  • Proficient in keeping batch record, writing SOPs.
Consultant Biological, 04/2017 - 08/2019
Johnson & Johnson Santa Rosa, CA,
  • Developed and starting fermentation team that involves setting up bioreactor (1L to 10L Eppindorff and Applikkon) and assisting in process development and data analysis in micro fermenters using genetically modified yeast strains for the production of enzymatic cocktails used for saccharification of lignocellulosic-biomass for biofuel production
  • Mid-size bench scale and mini bioreactor fermentation team lead in developing engineered yeast to produce enzymes for hydrolysis of lignocellulosic biomass: with expertise in fermentation and upstream process development in a high throughput fashion.
  • Optimized production in 10L bioreactors to determine best pH, temperature, DO%, duration of run, inoculation density, air flow rate, RPM.
  • Developed assay for high through put strain evaluation, media optimization, and process development in benchscale and mini scale and qualify all micro and mini fermenters in regular workflow
  • Proficient in tech transfer following ICH Q10 and quality by design guidelines, working with CROs writing process description, SOP, keeping batch record, validation protocol using OQ and PQ.
  • Actively working with interdisciplinary areas with strain engineers, data scientists, pilot plant, analytics, and downstream team.
  • Developed a successful team in an organization with leadership proficiency within remarkable time frame
Research Associate, 01/2015 - 02/2017
Instil Bio Tarzana, CA,
  • Design, set up and maintain bench scale bioreactor, 1L to 3L, for Pichia stipitis for ethanol production
  • Expertise in downstream biomass processing: cell harvest, homogenization, protein purification using differential centrifugation and TFF.
  • Protein purification: Anion exchange chromatography (FPLC AKTA), affinity chromatography (histag)
  • Experience in vector design and manipulation, over expression of genes of interest by promoter optimization, switching to strong constitutive promoter, or heterologous promoter. Manipulation with RBS optimization, codon optimization.
  • Proficient in keeping batch record, writing SOPs.
Research Associate I, 04/2014 - 12/2014
Covance Detroit, MI,
  • A key member in QC team: protein expression
  • Used a combination of HPLC, PAA gels for product analysis
  • Performed Monoclodal and Polyclodal Western Blotting
  • Responsible for expression of proteins of pathogens that were of national interest in partnership with the Center for Infectious Disease Research
  • Handled molecular biology techniques to clone target genes into in-house expression vectors for E. coli systems at high-throughput
  • Presented current research of scientific breakthroughs in infectious diseases field to scientific audiences
  • Successfully expressed novel proteins in bacterial hosts for novel studies of structure of medical-relevant viral diseases, such as Ebola.
  • Worked in coordination with cloning, purification, and crystallography groups in order to timely deliver protein targets
Internship Student, 08/2012 - 11/2012
Company Name City, State,
  • Researched for correlation of TOR signaling pathway and ER stress
  • Used Fluorescent Microscopy to observe vacuolar morphology changes in S. cerevisiae
  • Maintained records of morphologies
  • Reported research findings
  • Analyzed 126 non-essential gene delete strains of S. cerevisiae
  • Maintained detailed records of experiments to maintain accuracy of results
  • Independently performed the experiment, and developed troubleshooting capabilities to acquire data
  • Discussed data with researchers to help determine future strains to be analyzed
Education
Ph.D.: Biological Systems Engineering, Expected in 05/2023
-
Washington State University - Richland, WA,
GPA:
Status -
  • Awarded Fellow of the 2020 cohort for the Foundation for Food and Agriculture Research
  • Scholarship WSU Research Assistantship
Master of Science: Chemical And Biological Technologies, Expected in 02/2017
-
UNB - Universidade De Brasilia - Brasilia, Brazil,
GPA:
Status -
  • Dissertation: “Production of expansin protein BsEXLX1 in P. pastoris.”
Bachelor of Science: Biology With An Emphasis On Biotechnology, Expected in 08/2006
-
Brigham Young University - Idaho - Rexburg, ID
GPA:
Status -


  • Minor in Chemistry

Associate of Science: General Studies, Expected in 04/2010
-
LDS Business College - Salt Lake City, UT
GPA:
Status -
  • IC3 Certificate- Excel, Microsoft Word, PowerPoint proficiency
Professional Internship: Biochemistry, Molecular, Cellular And Biology, Expected in 12/2012
-
University of California - Davis - Davis, CA
GPA:
Status -
Accomplishments


Publications:


Karekar, Supriya, Jessica Stefanini, and Birgitte Ahring. "Homo-Acetogens: Their Metabolism and Competitive Relationship with Hydrogenotrophic Methanogens." Microorganisms 10.2 (2022): 397.


Conference Presentations:

  • Teixeira, T. Rodrigues, K. Mendes, T. Silva, C. Souza, A. Anastacio, G. Lo Sciuto, D. Lopes, R. Santana, M. Mello, V. Ribeiro, J. Gonçalves, S. Salum, T. Damaso, M. Siqueira, F. Rodrigues, D. Abdelnur, P. & Favaro, L. (2018, November). “Functional studies of Thermobifida fusca and Trichoderma reesei LPMOs produced by Komagataella phaffii and their application in saccharification of sorghum and sugarcane bagasse,” 2nd LPMO Symposium, Marseille, France.
  • Stefanini, R. Pacheco, T. Machado, F & Gonçalves, S. (2017, September). “Production of recombinant expansin BsEXLX1 in P. pastoris using glucose as carbon source”. XXI Simposio Nacional de Bioprocessos, Aracaju, Brazil.
  • Stefanini, R. Pacheco, T. Machado, F. & Gonçalves, S. (2017, February). “A factorial design analysis of growth kinetics of Pichia stipitis.” 39th Symposium on Biotechnology for Fuels and Chemicals, San Francisco, USA.


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Resume Overview

School Attended

  • Washington State University
  • UNB - Universidade De Brasilia
  • Brigham Young University - Idaho
  • LDS Business College
  • University of California - Davis

Job Titles Held:

  • PhD Graduate Assistant
  • Consultant Biological
  • Research Associate
  • Research Associate I
  • Internship Student

Degrees

  • Ph.D.
  • Master of Science
  • Bachelor of Science
  • Associate of Science
  • Professional Internship

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