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Senior Associate Scientist Resume Example

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SENIOR ASSOCIATE SCIENTIST
Experience
CelgeneCambridge, MASenior Associate Scientist11/2008 to Current
  • Promoted from Research Associate to Senior Research Associate
  • Developed TaqMan assays to screen for cytokine modulation in response to therapeutic exposure in pre clinical models.
  • Isolated RNA from tumor samples to screen for M1 and M2 macrophage gene expression in the tumor micro environment using Taqman.
  • Developed and validated immunoassays (ELISA, MesoScale and Luminex) to determine efficacy of lead drug candidates in cell lines, pre clinical models, human tissue biopsies and whole blood.
  • Transferred technology to CRO to be used screening clinical samples.
  • Responsible for determining drug occupancy in mouse, rat and monkey toxicology samples.
  • Utilized site-directed mutagenesis to create stable cell lines expressing clinically relevant mutations for drug screening.
  • Responsible for testing clinical biopsy samples to determine drug occupancy and reporting data across the organization.
  • Aided in clinical study protocol design for tissue sample preparation to be used in biomarker analysis of clinical compound.
Adnexus TherapeuticsWaltham, MASenior Research Associate04/2006 to 11/2008
  • Developed and validated ELISA assays used in primary drug efficacy screens 
  • Used the Luminex system to genotype SNPs associated with deep vein thrombosis.
  • Established SOPs for reagents to be used in commercial applications.
  • Aided in creating a GLP lab on site to screen clinical samples.
  • Completed necessary program to become GLP trained.
  • Executed experiments and wrote reports for stability studies, adhering to strict SOPs.
GenzymeCambridge, MaResearch Associate; Contract Position08/2005 to 04/2006
  • Evaluated effects of siRNA treatment on cancer cells' ability to survive and effects on downstream targets using Taqman.
  • Evaluated antibodies to be used in preclinical biomarker screening.
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Children's Hospital Oakland Research InstituteOakland, CAResearch Assistant lll09/2002 to 07/2005
  • Worked independently to develop a molecular-based assay to test for deletion of the alpha-globin gene to be used in a clinical setting.
  • Fostered collaborations with Los Alamos National Laboratories to create a Luminex- based assay to analyze various SNPs associated with thalassemias.
  • Studied lipid profiles in response to dietary modifications.
  • Established microlipid assay protocols for total cholesterol and triglycerides.
Sugen CorporationSouth San Francisco, CAResearch Assistant ll05/2001 to 09/2002
  • Utilized various cell and molecular biology techniques to validate signaling pathways as potential cancer targets.


Los Alamos National LaboratoryLos Alamos, NMResearch Assistant l06/1998 to 08/1998
  • Utilized flow cytometry to determine the binding properties of cholera toxin in an in vitro assay system.
Accomplishments
Developed primary screening assay used to validate clinical candidate through pre clinical and phase two clinical trials. Completed successful transfer of SOP to CRO.
Skills
  • Molecular Biology: RNA isolation from primary cells and tumor samples, RT-PCR, Taqman, genomic and plasmid DNA isolation, gene cloning, linker mediated PCR optimization, expression vector construction, site-directed mutagenesis, primer design, DNA sequencing analysis and siRNA.

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  • Tissue Culture: Experience with mammalian cell lines: RKO, Heks, Ramos, Jurkat, 293Ts, HeLa, HL-60's. Primary cell isolation: B-cells, PBMC's and T-cells. Transfections and stable cell line development.

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  • Protein Detection: Immunoassay development including troubleshooting and FDA validation, immunoprecipitation, western analysis, ELISA, MSD and Bio-Plex development and validation.

Education
Master of Science:Biochemistry and Molecular Biology2000University of Florida, Gainesville, FLCloned a novel gene from AT rich organism using linker mediated gene walking.
Bachelor of Science:Biochemistry1997California Polytechnic State University, San Luis Obispo, CA
Publications

1. Evans, E.K., Tester, R., Aslanian, S. et al. Inhibition of Btk with CC-292 provides early pharmacodynamic assessment of activity in mice and humans. J Pharmacol Exp Ther. 2013 Aug;346(2):219-28

 

2. Daniel W Pierce*,1, Sabine Ponader, PhD*,2, Kumudha Balakrishnan*,3, Varsha Gandhi, PhD3, William G. Wierda, MD*,4, Susan O'Brien, MD5, Carla Heise, PhD1, Mariana Nacht, PhD*,6, Sharon Aslanian*,6, Xiping Liu*,1, Sean Hong*,1, Shijuan Wu*,1, Marianna Zavodovskaya, MS*,1, Jeffrey Marine*,7, Evelyn Barnett*,7, Pilar Nava-Parada*,7, Jay Mei, MD7, Rajesh Chopra, MD7, Jan A. Burger, MD, PhD4, and Juswinder Singh*,6 Target Engagement, Pathway Inhibition, and Efficacy Of The Bruton's Tyrosine Kinase (Btk) Inhibitor CC-292.November 15, 2013; Blood: 122 (21)

 

3. Mamluk R1, Carvajal IM, Morse BA, Wong H, Abramowitz J, AslanianS, Lim AC, Gokemeijer J, Storek MJ, Lee J, Gosselin M, Wright MC, Camphausen RT, Wang J, Chen Y, Miller K, Sanders K, Short S, Sperinde J, Prasad G, Williams S, Kerbel R, Ebos J, Mutsaers A, Mendlein JD, Harris AS, Furfine ES.Anti-tumor effect of CT-322 as an adnectin inhibitor of vascular endothelial growth factor receptor-2. MAbs. 2010 Mar 25; 2(2)

 

4. Aslanian, S., Azimi, M., Noble, J., Hoppe, C. Application of flow cytometry-based genotyping for rapid detection of hemoglobin variants. Int J Lab Hematol. 2007 Aug;29(4):284-91 Malmuk et al.,  

 


Presentations
1. E. Evans, S. Ponader, R. Karp, R. Tester, M. Sheets, S. Aslanian, T. St. Martin, M. Nacht, Z. Zhu, P. Chaturvedi, S. Witowski, H. Lounsbury, K. Stiede, J. Burger, R. Petter, J. Singh, W.F. Westin. Covalent Inhibition of Btk with Clinical Development Compound AVL-292 Disrupts Signaling That Maintains the Microenvironment Necessary for Chronic Lymphocytic Leukemia Growth
2. Aslanian, S., Azimi, M., Hoppe, C. Rapid Detection of -thalassemia Mutations with the Luminex xMAP System. Newborn Screening and Genetic Testing Symposium, Portland Oregon
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Resume Overview

Companies Worked For:

  • Celgene
  • Adnexus Therapeutics
  • Genzyme
  • Children's Hospital Oakland Research Institute
  • Sugen Corporation
  • Los Alamos National Laboratory

School Attended

  • University of Florida
  • California Polytechnic State University

Job Titles Held:

  • Senior Associate Scientist
  • Senior Research Associate
  • Research Associate; Contract Position
  • Research Assistant lll
  • Research Assistant ll
  • Research Assistant l

Degrees

  • Master of Science : Biochemistry and Molecular Biology 2000
    Bachelor of Science : Biochemistry 1997

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