Microsoft Word, Excel, and PowerPoint
BD FACSDiva Software
Professional Experience
The Ragon Institute of MGH, MIT and HarvardOctober 2013 to CurrentResearch Technician, Lab Manager Cambridge, MA
Investigate the role PD-1 plays in T cell exhaustion in the context of HIV infection and how blocking this receptor and its ligands influences CD4+ and CD8+ T cell functions Perform functional intracellular cytokine assays and CFSE proliferation assays to determine the impact of blockade of PD-l1 and other inhibitory pathways such as IL-10 on restoring function of T cells and other cell subsets Execute qRT-PCR assays using Taqman and SYBR green technologies to determine the fold difference between mRNA transcripts up-regulated when PD-l1/il-10 blockade is applied Examine HIV- specific T cell responses through class I and II tetramer staining Responsible for ordering lab supplies and reagents on multiple different grants Process patient samples to isolate peripheral blood mononuclear cells (PBMCs) via ficoll separation; store samples using cryopreservation Stain primary leukocyte cells for extracellular and intracellular to be used for flow cytometry or live cell sorts Separate primary blood cell using magnetic bead isolation techniques Evaluate PBMC samples via flow cytometry using BD FACS Diva software Prepare and maintain sterile cell culture media and reagents Culture, maintain, freeze and thaw mammalian primary cells Perform DNA/RNA/mRNA extraction using standard techniques Train all new lab members including summer students in accordance with BL2+ safety practices Ensure quality control of equipment, reagents and cellular components.
The Caroll Center for the BlindJuly 2013 to CurrentNightshift Supervisor Newton, MA
Assure the safety and comfort of the clients during the evening and weekend hours Keep updated with the nurse's reports regarding each client's health concerns Prepare meals for each client at a timely manner Carefully assist each client with mobility Respond to emergency health situation of each client immediately and appropriately.
The Ragon Institute of MGH, MIT and HarvardMay 2013 to September 2013Research Intern Cambridge, MA
Performed scientific research experiments in an attempt to understand epitope presentation and processing by MHC 1 molecules in the context of HIV.
Used cell isolation techniques to purify PBMCs from blood (Ficoll), select monocytes and CD4 T cells, differentiate and harvest dendritic cells and macrophages.
Analyzed protein concentration in cellular extracts with BSA assay, and protein expression by Western Blotting.
Cultured and maintained B cells and cancer cells.
Xiao Lab University of Massachusetts AmherstSeptember 2011 to May 2013Student Research Assistant Amherst, MA
Utilized MTT assay on different cancer cells to determine the synergistic effect between different compounds synthesized in the lab.
Cultured and maintained cancer cells.
Compiled records and interpreted experimental and analytical results.
University of MassachusettsJanuary 2010 to May 2013Computer Consultant Amherst, MA
Maintained a friendly, approachable and creative demeanor while assisting students and faculty utilize OIT facilities.
Responsible for handling all emergencies, as well as, troubleshooting technical issues related to equipment malfunction and hardware/software issues.
Assisted faculty members with accessing specialized software, as well as, audio and video editing tools.
Enforced policies and procedures and provided outstanding customer service to facility users.
UMass Medical School, Matthews LabMay 2012 to August 2012Summer Research Intern Worcester, MA
Investigated the folding of FUS/TLS RRM and its role in ALS.
Used molecular Biology technique to sub-clone the gene of interest.
Monitored secondary and tertiary structural changes of the protein as a function of denaturant using circular dichroism (CD) and Tryptophan.
Broad Institute of MGH, MIT and Harvard, Garraway LabJune 2011 to August 2011Research Intern Cambridge, MA
Analyzed the signaling pathways that modified sensitivity to PLX4720 in BRAFV600E melanoma.
Used Western Blotting to check protein phosphorylation of different cell signaling pathways.
Cultured and maintained different melanoma cell lines.
Massachusetts General HospitalJune 2010 to August 2010Patient Ambassador Boston, MA
Assisted in discharge of patients from the hospital.
Assisted in escorting and transporting patients to their appointment.
Chelsea High SchoolJune 2010 to August 2010Teacher's Assistant Chelsea, MA
Helped teach high school summer students the basics of Biology and Chemistry.
Helped the youth become responsible and caring citizens.
Developed young people's capacity to engage in positive behaviors that nurture their own well being, set personal goals and live successful as self-sufficient adults.
Education and Training
University of Massachusetts Amherst5 2013Bachelor of Science: BiologyAmherst, MABiology
Skills
audio, BCA, CD, hardware, client, clients, customer service, functional, grants, Excel, PowerPoint, Microsoft Word, PAGE, PCR, policies, quality control, research, safety, scientific, troubleshooting, video editing, Western Blotting
