• High throughput/Automation equipment usage: Automated Liquid Handling (BioTek- MultiFlo), Robotic microplate handling (Orbitor- Thermo Scientific), High Content screening (CellInsight), Live C. elegans sorting using large particle flow cytometer
• Molecular biology: RT-PCR, PCR optimization, Cloning, DNA/RNA extraction, Western Blot, Transfection, Genotyping, RNA interference, primer design, gel electrophoresis, sample preparation for sequencing
• Cell culture/ Cell based assays: Culturing human fibroblasts, cell staining, cell counting, FACS analysis, cell viability assays, aseptic techniques, cryopreservation
• In-vivo experience: C.elegans (Culture, gene expression, behavioral assays), D.rerio (Genotyping) complying with IACUC guidelines
• Microscopy: Bright-field, Fluorescence microscopy, slide preparation, live cell in-vitro and in-vivo high content imaging
• Microbiology: MIC-MBC Tests, Gram Staining, Microbial identification using biochemical tests
• Software/Bioinformatics tools: Galaxy (Mapping DNA sequence & analysis of INDELS), GraphPad Prism, HCS CellInsight, Microsoft office, ImageJ, BioRender Illustrator, using NCBI database (including BLAST, GenBank, Pubmed)
GPA: 3.79
GPA: 3.45
Dean's List Honoree - Academic Year 2016
• Highly motivated researcher with 3 plus years of experience in biotechnology, cell biology and infectious diseases
• Performed molecular and cell-based assays to support research in the fields of HIV-1 and mitochondrial medicine
• Furthered preclinical studies in C. elegans models of Down Syndrome including assay development and troubleshooting
• Conducted high throughput screens assaying mitochondrial physiology in cells and C. elegans models of mitochondrial diseases
• Proficient in using automated liquid handlers, plate movers and high content screening platforms
• Effective team member with excellent written/oral communication skills and the ability to summarize research findings
• Planning and prioritizing experiments to conduct drug screens assessing the therapeutic efficacy of lead single and combinatorial therapies for mitochondrial diseases in C. elegans models of Down syndrome
• Using high throughput screening approaches to study mitochondrial physiology to measure mitochondrial unfolded protein response through reporter gene, membrane potential, and mitochondrial content in C. elegans models of mitochondrial diseases
• Developing and troubleshooting high content cellular imaging analysis in human fibroblasts with mitochondrial deficiencies
• Liaising on mitochondrial research with pharmaceutical companies for conducting therapeutic compound screens for pre-clinical studies in C. elegans
• Culturing human primary fibroblasts and performing FACS analysis with an emphasis on mitochondrial biology
• Collaborating with cross functional research teams for modeling mitochondrial diseases in C. elegans
• Carrying out DNA extraction and genotyping in D. rerio model of mitochondrial dysfunction
• Preparing reagents for the lab when required, writing and protocols to support assay documentation
• Optimized PCR conditions and designed primers to study the transcriptional profile in latent HIV-1 infection using U1 promonocytic cell line as a model
• Cultured U1 and HEK293 cells, performed RNA extraction and TA cloning of HIV-1 PCR products
• Worked closely with fellow members to develop an understanding on plasmid construction, T-cell biology, transfection of HEK293
• Assessed the antioxidant property of phenols from Cocos nucifera by performing colorimetric assays
• Optimized reaction conditions for solvent extraction using response surface methodology
• Analyzed the active fraction with high phenolic content by performing column chromatography and purification using HPLC
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