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Research Technician III- Mitochondrial Medicine Resume Example

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RESEARCH TECHNICIAN III- MITOCHONDRIAL MEDICINE
Skills

• High throughput/Automation equipment usage: Automated Liquid Handling (BioTek- MultiFlo), Robotic microplate handling (Orbitor- Thermo Scientific), High Content screening (CellInsight), Live C. elegans sorting using large particle flow cytometer
• Molecular biology: RT-PCR, PCR optimization, Cloning, DNA/RNA extraction, Western Blot, Transfection, Genotyping, RNA interference, primer design, gel electrophoresis, sample preparation for sequencing
• Cell culture/ Cell based assays: Culturing human fibroblasts, cell staining, cell counting, FACS analysis, cell viability assays, aseptic techniques, cryopreservation
• In-vivo experience: C.elegans (Culture, gene expression, behavioral assays), D.rerio (Genotyping) complying with IACUC guidelines
• Microscopy: Bright-field, Fluorescence microscopy, slide preparation, live cell in-vitro and in-vivo high content imaging
• Microbiology: MIC-MBC Tests, Gram Staining, Microbial identification using biochemical tests
• Software/Bioinformatics tools: Galaxy (Mapping DNA sequence & analysis of INDELS), GraphPad Prism, HCS CellInsight, Microsoft office, ImageJ, BioRender Illustrator, using NCBI database (including BLAST, GenBank, Pubmed)

Education and Training
12/2018
Master of Science - Infectious Disease
Drexel University College of MedicineCity

GPA: 3.79

  • Completed coursework in Immunology, Clinical correlations in Infectious diseases and Immunology and Advanced Cell and Molecular Biology
2017
Bachelor of Technology - Biotechnology
SASTRA University

GPA: 3.45

Dean's List Honoree - Academic Year 2016

Summary

• Highly motivated researcher with 3 plus years of experience in biotechnology, cell biology and infectious diseases
• Performed molecular and cell-based assays to support research in the fields of HIV-1 and mitochondrial medicine
• Furthered preclinical studies in C. elegans models of Down Syndrome including assay development and troubleshooting
• Conducted high throughput screens assaying mitochondrial physiology in cells and C. elegans models of mitochondrial diseases
• Proficient in using automated liquid handlers, plate movers and high content screening platforms
• Effective team member with excellent written/oral communication skills and the ability to summarize research findings

Experience
Research Technician III- Mitochondrial Medicine Penn State University | University Park , PA04/2019 - Current

• Planning and prioritizing experiments to conduct drug screens assessing the therapeutic efficacy of lead single and combinatorial therapies for mitochondrial diseases in C. elegans models of Down syndrome
• Using high throughput screening approaches to study mitochondrial physiology to measure mitochondrial unfolded protein response through reporter gene, membrane potential, and mitochondrial content in C. elegans models of mitochondrial diseases
• Developing and troubleshooting high content cellular imaging analysis in human fibroblasts with mitochondrial deficiencies
• Liaising on mitochondrial research with pharmaceutical companies for conducting therapeutic compound screens for pre-clinical studies in C. elegans
• Culturing human primary fibroblasts and performing FACS analysis with an emphasis on mitochondrial biology
• Collaborating with cross functional research teams for modeling mitochondrial diseases in C. elegans
• Carrying out DNA extraction and genotyping in D. rerio model of mitochondrial dysfunction
• Preparing reagents for the lab when required, writing and protocols to support assay documentation

Graduate Student ResearcherDrexel University College Of Medicine | City , STATE11/2017 - 12/2018

• Optimized PCR conditions and designed primers to study the transcriptional profile in latent HIV-1 infection using U1 promonocytic cell line as a model
• Cultured U1 and HEK293 cells, performed RNA extraction and TA cloning of HIV-1 PCR products
• Worked closely with fellow members to develop an understanding on plasmid construction, T-cell biology, transfection of HEK293

Undergraduate ResearcherSASTRA University | City , STATE07/2015 - 05/2017

• Assessed the antioxidant property of phenols from Cocos nucifera by performing colorimetric assays
• Optimized reaction conditions for solvent extraction using response surface methodology
• Analyzed the active fraction with high phenolic content by performing column chromatography and purification using HPLC

Activities and Honors
American Society for Microbiology (2018) ¨ Teaching/ Mentoring: Conducted weekly recitations and laboratory sessions in Fundamentals of Physics (2017-2018)
Peer-Reviewed Publications
  • M Venkitasubramani, S Ganesh, V Vellingiri; ‘Optimization of recovery of antioxidants from coconut shell using Response Surface Methodology'; J. Biomed. Ther. Sci., 2017, 4(1), 27-33.
  • Smruthi Ganesh, M Venkitasubramani, V Vellingiri, S Savariraj, R Perumal, B Pemiah; ‘Anti-Diabetic Potential of Selected Indian Traditional Medicinal Plants – An Updated Review'; J. Pharm. Sci. & Res. Vol. 8(10), 2016, 1144-1158
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Resume Overview

School Attended

  • Drexel University College of Medicine
  • SASTRA University

Job Titles Held:

  • Research Technician III- Mitochondrial Medicine
  • Graduate Student Researcher
  • Undergraduate Researcher

Degrees

  • Master of Science - Infectious Disease
    Bachelor of Technology - Biotechnology

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