-Dexterous and dynamic Research Scientist with hands on experience working in an industrial and academic setting
-Highly result oriented along with proven ability to multitask and prioritize tasks to meet deadlines
-Proficient in MS WORD, EXCEL and familiar with biostatistics software
-Excellent communication skills and experience working in both, horizontal as well as vertical groups
Summary of Skills
MS Windows, MS Word, MS Excel, Microsoft Outlook, SPSS,
EXTRA CURRICULAR & VOLUNTEERING
Residence Safety Office, Northeastern University 01/12-08/13
InstituteMaster of ScienceBiotechnologyBiotechnology
1 2013Northeastern University － Boston, USBachelors of Pharmacy
1 2010Pune University － Pune, India
Research Technician II, 09/2013 － CurrentMassachusetts General Hospital, Cancer Center , Ellisen Lab
Assisted in studying the molecular mechanism of Redd1 mediated metabolism Studied protein expression under impaired autophagy in Redd1 null mice using western blots Analyzed levels of autophagy markers under basal and stress conditions in Redd1 null mice using calorimetric assays, fluorescence assay and spectrophotometric assays Studied LC3 pucta formation in GFP transfected cells under basal & stress condition in REDD1 wild type & null cells Analyzed dysfunctional mitochondria using flow cytometry Isolated RNA & compared mitochondrial & Nuclear protein mRNA levels using qPCR in REDD1 Wild type & Null samples Assisted in successfully reinserting the Redd1 gene to rescue Redd1 knockout cells Assisted in performing metabolomic study to elucidate the role of Redd1 in pancreatic cancer Successfully designed & optimized primers to genotype large cohorts of mice to maintain the gene of interest Processed human blood samples as a part of an ongoing clinical trial and established a database from their results Successfully established primary cell lines from pancreatic tumors to access levels of various biomarkers in pancreatic tumor progression in a REDD1 null background.
Research Intern, 05/2012 － 01/2013Tufts University, School of Dental Medicine
Studied the temporal expression of RB1 mRNA in wild type mice to better understand the role of RB1 in dental stem cell differentiation using immunohistochemistry on formalin fixed and frozen tissue sections.
Performed a forward ENU screen on zebrefish and successfully identified a phenotype mutant with mineralization defect which could be eventually translated into human models for craniofacial, dental or skeletal disorders.
Successfully bred and maintained large shoals of zebrafish.
Participated and often presented my work in weekly meetings.
Research Assistant, 11/2010 － 06/2011Centaur Pharmaceuticals
Assisted in developing generic formulations for oral dosage forms implementing the concepts of Quality by Design.
Successfully upscaled the protocol for multiple formulations in order to transfer it to the production department.
Performed accelerated stability studied and accurately determined the shelf life for various formulations.
Efficiently maintained an inventory for API and excipients.
Assisted in writing/ updating SOP's.
Formulation R&D Department
Molecular Biology Nucleic Acid Isolation Nucleic Acid Quantification Gel Electrophoresis Forward ENU Screening Animal Tissue Harvesting Alizarin Red Staining Alician Blue Staining PCR RT-qPCR qPCR Florescence Assays Tissue Fixation Cryopreservation Genotyping Cloning Transformation Transfection Double Digestion Diagnostic Digestion Mini Preps Gel Purification Protein Biochemistry Protein Extraction Protein Quantification (BCA) Western Blotting SDS-PAGE ELISA Spectrophotometric Assay Cell Biology Immunofluorescence Staining Fluorescence Microscopy Flow Cytometry Insitu Hybridization Cell Culture Establish Primary Cell Cultures Freeze/Thaw Cell Lines Mammalian Cell Culture Bacterial Cell Culture Thesis, Pune University 12/09-05/10 Successfully completed a thesis on 'Development of an Economic Culture Media for Plant Tissue Culture' which was recognized among the top ten dissertations in the university for that academic year Publication: A REDD1/TXNIP complex regulates ROS to control ATG4B activity and stress-induced autophagy, Nature Cell Biology (Under Review) Posters: Recent Advances in Chemotherapy-A Review Mainly Focusing on Nano-Technological Methods Used in Cancer Treatment Enzyme Immobilization in Organogels.
Responsible for the safety of undergraduate students living in school dorms Attending to queries during moving-out/ moving-in sessions Maintain a written record of visitors in the dorm Student member of the Indian Pharmaceutical Association 05/08-04/10 Member of the General Student Body 09/09-05/10 Organized annual sports meet on an intra college level Organized college festivals and annual cultural gatherings
API, oral, database, forms, inventory, meetings, access, MS Excel, Office, Microsoft Outlook, MS Windows, MS Word, Quality, Safety, SOP, SPSS, type
Proctor Responsible for the safety of undergraduate students living in school dorms Attending to queries during moving-out/ moving-in sessions Maintain a written record of visitors in the dorm Student member of the Indian Pharmaceutical Association 05/08-04/10 Member of the General Student Body 09/09-05/10 Organized annual sports meet on an intra college level Organized college festivals and annual cultural gatherings
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Companies Worked For:
Massachusetts General Hospital, Cancer Center , Ellisen Lab
Tufts University, School of Dental Medicine
Formulation R&D Department
Job Titles Held:
Research Technician II
Master of Science Biotechnology Bachelors of Pharmacy
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