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research technician b promoted to research technician d resume example with 19+ years of experience

Jessica Claire
  • Montgomery Street, San Francisco, CA 94105 609 Johnson Ave., 49204, Tulsa, OK
  • H: (555) 432-1000
  • C:
  • resumesample@example.com
  • Date of Birth:
  • India:
  • :
  • single:
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Profile

Experienced senior research technician with excellent technical, analytical, and teaching skills demonstrated by 13 years of experience.

Skills
  • Cell and Tissue Culture
  • Nucleic acids and protein extraction
  • Design primers and probes
  • PCR, RT-PCR, Realtime/Quantitative PCR, Copy number assays
  • Western blotting, Immunohistochemical staining
  • Fluorescence in situ hybridization
  • DNA sequencing and data analysis
  • Transformation, Transfection and Cloning
  • Functional Tumorigenic assays
  • In vitro cytotoxicity assays
  • In vitro differentiation assays
  • Flow cytometry and analysis on Flowjo
Accomplishments/ Published Papers
  • Ganglioside GD2 expression is maintained upon recurrence in patients with osteosarcoma. Clinical Sarcoma Research, 2015 Jan 24;5(1):4
  • Development of a Model System to Evaluate Local Recurrence in Osteosarcoma and Assessment of the Effects of Bone Morphogenetic Protein-2. Clinical Cancer Research, 2014 Dec 3
  • Insulin-like growth factor 1 receptor and response to anti-IGF1R antibody therapy in osteosarcoma. PLoS One, 2014 Aug 29;9(8)
  • HER-2 expression is not prognostic in osteosarcoma; a Children's Oncology Group prospective biology study. Pediatrics Blood Cancer, 2014 Sep;61(9):1558-64
  • Ganglioside GD2 as a therapeutic target for antibody-mediated therapy in patients with osteosarcoma. Cancer, 2014 Feb 15;120(4):548-54
  • B-Catenin Does Not Confer Tumorigenicity When Introduced into Partially Transformed Human Mesenchymal Stem Cells. Sarcoma, 2012;2012:164803
  • Cell surface receptor expression patterns in Osteosarcoma. Cancer. 2011 Jul 12.
  • Preclinical activity of palifosfamide lysine (ZIO-201) in pediatric sarcomas including oxazaphosphorine-resistant Osteosarcoma. Cancer Chemother Pharmacol. 2009 Sep; 64(4): 733-40. Epub 2009 Feb 18.
  • Activation of the RAF/MEK/ERK pathway mediates apoptosis induced by chelerythrine in osteosarcoma. Clin Cancer Res. 2008 Oct 15; 14(20): 6396-404.
  • Platelet-derived growth factor receptor as a prognostic marker and a therapeutic target for imatinib mesylate therapy in Osteosarcoma. Cancer. 2008 May 15; 112(10): 2119-29.
  • Monoclonal antibodies to Mycobacterium tuberculosis CDC 1551 reveal subcellular localization of MPT51. Tuberculosis (Edinb). 2007 Nov;87(6):489-97. Epub 2007 Sep 19.
  • Antigenic Evidence of Prevalence and Diversity of Mycobacterium Tuberculosis Arabinomannan. J Clin Microbiol. 2004 Jul;42(7):3225-31.

Professional Experience
Research Technician B promoted to Research Technician D , 2005 - Current
Albert Einstein College Of Medicine Bronx, NY,

Lab manager at Dr Richard Gorlick Lab

Department of Pediatrics (Hematology/Oncology)

  • Derive primary cell lines from freshly resected osteosarcoma tissue
  • Children’s Oncology Group (COG) osteosarcoma tissue bank – maintenance and distribution of established cell lines, and cell preparation for xenograft injections
  • Identification of an osteosarcoma progenitor cell by differentiation of human mesenchymal stem cell to osteoblast by Flow Cytometry
  • Analyze different signal transduction pathways by immunoblotting and cytotoxicity assays to detect the effects of different chemical compounds/drugs in Osteosarcoma samples
  • Generate SAGE libraries from primary Osteosarcoma samples
  • Determine the genomic amplification of IGF1-R in Osteosarcoma
  • Introduce different genetic elements into human mesenchymal stem cell in order to transform into Osteosarcoma
  • analyze data, develop recommendations and action plans, and new approaches to obtain needed information
  • Oversee the whole laboratory, train students and fellow researchers
  • Monitor inventory and place orders

Research Technician B, 10/2002 - 2005
Jones Lange Lasalle Inc. Carlsbad, CA,

Research Technician at Dr. Arturo Casadevall Lab

Department of Pediatrics (Microbiology and Immunology)

  • Assist in the projects involving the development of carbohydrate conjugate vaccine for Mycobacterium Tuberculosis
  • Perform various ELISA assays, both qualitatively and quantitatively, to determine the presence of antigens and antibodies in patient samples
  • Work at BSL 3 environment for different assays involving live MTb strains
  • In-vivo characterization of monoclonal antibodies to various MTb strains
  • Maintain stocks of hybridoma cell lines
  • Familiar with techniques involving monoclonal antibody production

Research Intern , 2002 - 07/2002
Seattle Children's Hospital Bellevue, WA,

Research Intern at Dr. Peter Rabinovitch Lab

Department of Pathology

  • Telomere Length Assessment in G6 Telomerase knock out mouse tissues
  • Presented on HHMI poster symposium: “Chromosomal Instability in the Telomerase-Deficient Mouse”.

Student Helper, 09/2001 - 05/2002
Puc National Burbank, CA,

Student helper at Hematology Lab

  • Prepare solutions and media using sterile techniques
  • Ensure compliance with research protocols
  • Autoclave glassware and laboratory equipment
  • Pour agar plates
  • Responsible for laboratory management, stock evaluation, and order management

Tutor, 07/1999 - 11/1999
Onondaga Community College City, STATE, Myanmar

Tutored college students in Math and Science at Content Tutoring Center.

Education and Training
Bachelor's Degrees: Biochemistry, Expected in 08/2002
-
University of Washington - Seattle, WA
GPA: GPA: 3.2 /4.0
Status -

Biochemistry GPA: 3.2 /4.0


Associate of Science Degree: Mathematics/Science, Expected in 12/1999
-
Onondaga Community College - Syracuse, NY
GPA: GPA: 3.93/4.0
Status -

Mathematics/Science GPA: 3.93/4.0


Affiliations

American Association for Cancer Resesarch

Work History
Research Technician D, 06/2012 - Current
Albert Einstein College of medicine Bronx, NY
Research Technician B, 10/2002 - 06/2012
Albert Einstein College of Medicine Bronx, NY
Certificates and Trainings

  • Charge of Chemical Laboratory (C14), NYC Fire Department
  • Supervision of Commercial Cyrogenic Systems and for Storage and Handling of Cyrogenic Liquids (G97), NYC Fire Department
  • Certificate of Completion: 2 hour Lab Safety Training, Triumvirate Environmental
  • Biosafety Level II Awareness Training, Albert Einstein College of Medicine: Department of Environmental Health and Safety
  • Use of Barrier Maintained Rodents, Albert Einstein College of Medicine: Institutional Animal Care and Use Committee
Skills

MS Word, Excel, PowerPoint, EndNote, FlowJo Flow Cytometry Software, BD FACSDiva Software.



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Resume Overview

School Attended

  • University of Washington
  • Onondaga Community College

Job Titles Held:

  • Research Technician D
  • Research Technician B

Degrees

  • Bachelor's Degrees
  • Associate of Science Degree

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