Talented research scientist with strengths in communication and collaboration. Offering 9 + years of success in intensive research environments studying neurobiology and cellular aging. Strategic thinker
with proven ability to proactively lead effective teams that meet team, individual, and management objectives. Committed to identifying and leveraging opportunities for growth.
Skills
Microscopy (Fluorescent, compound, confocal),
Metamorph imaging software
Spectrofluorimetry,
Cell Staining and viability assays
RT-PCR/ QPCR
Molecular cloning
Protein/DNA/RNA extraction
Western Blot,
Flow Cytometry
C. Elegans lifespan/healthspan assays
C. elegans lifespan and healthspan quantification,
RNAi screening
Microbial culturing and transformations
Microfluidics
Transfection/Infection(viral)
Animal (rodent) handling and crosses
Intracranial Injection (mouse)
DNA sequencing/processing
RNA seq (Next Generation Sequencing)
MS Office Suite, Python, MATLAB, Graphpad Prism, ImageJ
Equipment maintenance and repair
MATLAB
Experience
Research Technician, 03/2014 to Current Basf – Caldwell - Tx, TX,
Conduct research experiments for the lab across multiple projects; design experimental projects while both supporting and reporting to Principal Investigator.
For NASA, collaboratively worked with the ESA (European Space Union) to launch C.
Elegans to space on the Falconx 9 rocket and the first to identify the effects of space environment on aging of individual neurons of various types.
First to comprehensively establish C.
Elegans as a model to study the molecular pathways activated by aerobic exercise and its effects on gene expression, cellular metabolism, and health of individual tissues and systemic health.
Co-managed and lead a team of technicians and undergraduate interns to perform preclinical lifespan experiments as part of a multi-institutional project funded by the National Institute of Aging to assess the effects of various compounds.
Utilize spinning-disc confocal microscopy to identify the effects of various stressors on mitochondrial health in multiple tissue types (including individual neurons and muscle cells).
Lead equipment maintenance and repair processes for entire laboratory.
Create novel genetic mutant strains of C.
Elegans to improve our understanding of the effects of single mutations on neuronal aging.
Study and characterize the effects of protein aggregation on neurodegeneration in C.
Elegans.
Quantify and qualify the morphological and metabolic effects of neuronal aging.
Handle time-lapse video imaging in conjunction with intracellular staining techniques of neuroblastoma cells (both mouse and human) to study and characterize extracellular vesicle dynamics in vitro.
Research Technician, 12/2013 to 05/2014 Basf – Cincinnati, OH,
Conducted DNA Sequencing, Processing and Post Reaction Purification to improve DNA sequencing quality and throughput.
Performed sales-related tasks to generate new customers.
Ran projects using ABI 3730xl DNA Sequencer.
Research Technician/Lab Manager, 09/2010 to 10/2013 MEMORIAL SLOAN KETTERING – City, STATE,
PI: Jason Huse.
Performed RT-PCR and QPCR and analyzed the results to identify genes targeted by therapeutic microRNAs for Glioblastoma Multiforme tumor (GBM).
Handled microbial Culturing and manipulation (transformations, mini/maxi prep); gel electrophoresis (genotyping/cloning/DNA extraction via gel purification); protein/DNA/RNA extraction (from frozen brain tissue/ cell culture/ mouse tail).
Handled lab maintenance and management, including equipment maintenance and repair, vendor interface, ordering and inventory of lab supplies.
Conducted Western Blot experiments to measure proteins overexpressed in GBM as well as the effectiveness of therapeutic miRNAs.
Performed cell growth and survival assays, DNA/RNA/protein extraction, generated hybridomas, and analyzed drug treatment and response (i.e w/ Gleevec) on GBM, Team Lead of Rutgers MBS Externship Exchange with Colgate Palmolive.
Education and Training
Masters of Business And Science (MBS): Biotechnology & Genomics, Expected in 05/2020 The State University of New Jersey - New Brunswick, NJ, GPA:
Bachelor of Arts (BA): Cell Biology And Neuroscience ; Nutrition, Expected in 05/2010 Rutgers, The State University of New Jersey - New Brunswick, NJ GPA:
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