Research Specialist Resume Example

Project 1: Quantifying Environmental Variables affecting Airborne Influenza Transmission.

• Understanding individual environmental variables like temperature, humidity and airflow in transmission of influenza virus using Guinea Pig as model animal.
• Performing experiments to understand influenza virus transmission in Guinea Pig model in environmental chambers.
• Hemagglutination, plaque and focus forming assays to quantify influenza viruses.
• Neuraminidase assay to quantify the neuraminidase activity of various influenza viruses.
• Maintaining mammalian cells in culture in BSL2 facilities.
• Propagation of Influenza Virus in embryonated eggs and cell cultures and Plaque purification of influenza viruses.
• Immunostaining.
• Transfection, Virus neutralization assays.
• Worked with Aerodynamic Particle Sizer (APS) to measure the size of particles.

Project 2: Visualization of virus site deposition in infected lungs using clearing protocols combined with different microscopy techniques.

• Cryopreservation of guinea pig respiratory tract infected with fluorescent influenza virus for cryomicrotome.
• PARS tissue clearing procedure for Guinea Pig lungs.
• Immunohistochemistry staining.
• Working with Stereomicroscopy, Light Sheet Microscopy, Confocal Microscopy and Multi-photon Microscopy to view the fluorescence in lungs.

Project 3: Generation of Primary cell lines from Guinea Pig Respiratory tissues.

• Establishing cell lines from guinea pig trachea, for in-vitro studies.
• Flow cytometry.
• Extraction of DNA and RNA.
• Molecular Biology techniques-PCR, qPCR, SDS PAGE, Electrophoretic Techniques, Immunological techniques like RIA, Immunosorbent Assay (ELISA), Western Blot.

Lab Manager Responsibilities

• Managing laboratory functions – inventory of lab supplies, vendor management, cytotoxic waste management and equipment maintenance.
• Assigned as the Lab Safety Officer to work with EHS.
• Trained and supervised associates for laboratory experiments.
• Certified in shipping 6.2 Infectious Substances, Biological Specimens, Dry Ice and Related Materials.
• FDNY C-14 certificate of fitness holder.
• Informing PI's and Laboratory Supervisors of chemical related health and safety requirements and assisting with the selection of appropriate safety controls.
• Working with researchers to develop SOPs for processes using hazardous chemicals.
• Ensuring laboratory workers receive chemical and procedure specific training.
• Conducting periodic inspection and taking immediate steps to abate hazards.
• Review and approve hazardous substances and fume hood for multiple protocols.
• Annually reviewing and evaluating the effectiveness of Laboratory Safety manual.

• Managing laboratory functions – inventory of lab supplies, vendor management, cytotoxic waste management and equipment maintenance.
• Assigned as the Lab Safety Officer to work with EHS.
• Trained and supervised associates for laboratory experiments.
• Certified in shipping 6.2 Infectious Substances, Biological Specimens, Dry Ice and Related Materials.
• FDNY C-14 certificate of fitness holder.
• Informing PI's and Laboratory Supervisors of chemical related health and safety requirements and assisting with the selection of appropriate safety controls.
• Working with researchers to develop SOPs for processes using hazardous chemicals.
• Ensuring laboratory workers receive chemical and procedure specific training.
• Conducting periodic inspection and taking immediate steps to abate hazards.
• Review and approve hazardous substances and fume hood for multiple protocols.
• Annually reviewing and evaluating the effectiveness of Laboratory Safety manual.