R&D Scientist with over 15 years of experience in both academic and commercial enterprises Peer-recognized expert in Molecular and Cellular Biochemistry applied to human diseases Principal investigator / scientist in basic and translational research projects totalizing ~ 3.5 million US dollars Experienced in training and supervision of staff and in coordinating productive collaborations with cross-functional teams from within housing institutions, as well as with external professionals Proven track record of scientific success as evidenced by first authorship of 5, co-authorship of 3 publications in high-impact journals.
DNA/RNA isolation, PCR amplification, qRT-PCR, cloning, Microarray sample preparation, sample preparation for NGS systems, ECIS (TEER), VenaFlux, Protein expression and isolation, Western blotting, ELISA, LUMINEX (Bioplex 200 system), IP/CO-IP, pull-down assays, Intracellular signaling, Signal transduction, Live protein-protein interaction, Immuno-staining Immunofluorescence/Immunohistochemistry), Multicolor Flow Cytometry - FACS analysis (surface and intracellular staining), siRNA/RNAi transfection, lenti/adeno viral preparation and transduction.
Expert in in vitro, ex vivo and in vivo assay systems: embryonic pluripotent cell culture and differentiation in to specific cell types, primary cell isolation (immune and vascular cells) from lungs, organ culture; en-face staining, retina isolation/staining; transgenic/inducible/disease-based animal models, animal husbandry/ procedures,
Genotyping, tail-cuff blood pressure measurements and in vivo bioassays; Drug testing, molecular screening, genetic complementation and in vivo validation studies using Zebrafish.
Advanced and experienced imaging/quantification scientist: FlowJo, Alpha imager, Phase contrast/inverted/fluorescent microscopes, epifluorescent stereoscopes and confocal microscopy; Image J, Metamorph, Molecular imaging and NIS-Elements microscope imaging software.
Proficient in: MS Word, Excel, PowerPoint, Access, Outlook; Systat SigmaPlot; GraphPad Prism; Schrödinger PyMol; Adobe reader, Photoshop, Illustrator.
Research Scientist07/2013 to CurrentBlood Research InstituteMilwaukee, WI
Manage research project aimed at identifying the
mechanisms involved in hematologic disorders, cancer and
cardiovascular diseases (CVD).
Discovered novel functions of a small GTPase and
its therapeutic potential in arresting cancer growth.
Comprehensively conducted nonclinical research
studies involving human/ non-human systems and disease models: sepsis,
inflammation, permeability, Type-2 diabetes and tumor.
Extensive experience in isolating and culturing cancer
cells (melanoma); Primary cells: endothelial, smooth muscle and immune cells.
Form and coordinate an extensive network of
collaborators who provide additional expertise.
Trained over 15 scientists, 2 postdoctoral
fellows, 40 graduate students and 12 research technologists.
Published 4 peer-reviewed articles and presented
at several international meetings.
Postdoctoral Associate09/2006 to 07/2013Blood Research InstituteMilwaukee, WI
Execute research project focused on physiological and pathological (tumor) angiogenesis.
Expert in neovascularization research by utilizing human and non-human systems.
Project lead to identify novel molecules that regulates cancer and blood pressure (hypertension).
Research Scientist07/2004 to 08/2006Osho Biotech Research InstituteIndia
Manage research project aimed at identifying genetic mutations in Hypertrophic Cardiomyopathy patients, with the goal of gene therapy based heart failure prevention.
Extensive experience in culturing mammalian cells; pluripotent embryonic carcinoma cell line, embryoid bodies and successfully differentiating into beating cardiomyocytes.
Research Technologist06/1998 to 08/2000Department of Biochemistry, Madurai Kamaraj UniversityIndia
Successfully produced modified embryonic cardiac stem cells that express skeletal Myosin binding protein-C gene (MyBP-C) to replace mutated cardiac MyBP-C.
The long-term goal of this project is to prevent heart failure in cardiomyopathy patients by gene therapy.
Ph.D.: BiochemistryMay 2006Madurai Kamaraj University (MKU) and Unité INSERM 582, Institut de MyologieIndia and France
Masters of Science (BS)Apr. 1998Madurai Kamaraj University (MKU)India
Bachelor of Science (BS)Apr. 1996Madurai Kamaraj University (MKU)India
Able to work in teams, to coordinate efforts with peers or to delegate, and supervise when acceptable to accomplish goals timely in multiple projects.
*Strong analytical and problem-solving abilities, as evidenced by the content and depth of the scientific production.
*Strong oral communication skills, as recognized by peers at international conferences and by trainees.
*Strong written communication skills, as evidenced by research products (peer-reviewed articles and grant proposals).