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Research Fellow Resume Example

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RESEARCH FELLOW
Professional Overview

Organized postdoctoral scientist specialized in biochemistry and molecular biology, genetic regulation of secondary metabolism and fungal biology. Adept and successful in writing grant proposals and manuscripts.

Core Qualifications
  • Cell culture, cell line generation and cell-based assay
  • Mammalian cell culture including embryonic stem cells, iPS cells, neuronal progenitor cells, primary neurons, cancer cell lines, isolation and immortalization of mouse embryonic fibroblasts
  • CRISPR/Cas9-mediated genome editing, shRNA-mediated gene silencing
  • Reporter gene construction and lentiviral transduction
  • Imaging and analytical techniques
  • Immunohistochemistry, confocal microscopy, PALM MicroBeam microdissection platform, Oddyssey Infrared imaging system
  • High-throughput FACS analysis, ELISA, quantitative RT-PCR
  • HPLC, GC/LC-MS, Chromatography (ion exchange, size exclusion)
  • Statistical analysis and computer software skills
  • Proficient in statistical analysis using GraphPad PRISM and R
  • Proficient in image analysis using ImageJ, CellProfiler, and confocal microscope software (quantification, colocalization, Z-stack)
  • Proficient in gene ontology and pathway analysis using genomics and proteomics data
Accomplishments
  • Trained and supervised undergraduate researchers and research associates.
  • Teaching assistant for Biochemistry class (2001, 2004).
  • Teaching assistant for Biochemistry laboratory (2000).
Education
Ph.D : Biochemistry and Molecular Biology, 2007 University of Minnesota － Minneapolis, MN GPA: Dr. Frederick J. Bollum Research Award (2006) Graduate Student Travel Award (2006, 2004) Summer Research Fellowship in Structural Biology Biochemistry and Molecular Biology Dr. Frederick J. Bollum Research Award (2006) Graduate Student Travel Award (2006, 2004) Summer Research Fellowship in Structural Biology
M.A : Biochemistry, 2002 Biochemistry
B.A : Genetic Engineering, 1999 Chonnam National University South Korea Genetic Engineering Tuition Scholarships (1996-1999)
Experience
Research Fellow
January 2007 to Current Harvard Medical School － Boston
  • Studied molecular mechanisms of brain aging and Alzheimer's disease focusing on DNA damage repair, cell cycle, and cell death pathway and epigenetic regulation.
  • Performed CRISPR-mediated genome editing to correct disease-associated mutations and SNPs in iPS cells and iPS-derived neural progenitor cells.
  • Contributed to team projects by providing expertise in gene silencing, CRISPR-mediated genome editing, protein expression, cell line generation, and immunohistochemistry.
  • Obtained and managed clinical samples from various organization.
Research Assistant
January 2002 to January 2007 University of Minnesota
  • Studied chromatin structure and expansion mechanism of repetitive DNA elements that cause polyglutamine diseases.
  • Broadened knowledge on various rare genetic disorders and neurodegenerative diseases.
  • Designed a screening method to identify specific DNA binding proteins and molecular biology techniques to characterize the function of protein and local chromatin structure.
  • Managed laboratory safety procedures and hazardous materials including radioactive isotopes.
Research Assistant
January 2000 to January 2002 Chonnam National University
  • Studied enzyme kinetics and lipid metabolism.
  • Performed molecular cloning, site-directed mutagenesis, protein expression and purification, and enzyme assays.
  • Maintained analytical instruments including HPLC, FPLC and spectrophotometer.
  • Managed laboratory inventories and safety procedures.
Research Assistant
January 1998 to January 2000 Pohang University of Science and Technology
  • Studied the genetics of plant senescence.
  • Screened mutant plants with longer longevity and performed genetic mapping and positional cloning using RFLP and other SNP detection methods.
  • Performed biochemical studies to characterize the function of cloned genes.
Publications
Presented a poster in Yeast Genetics Meeting, University of Washington, Seattle WA (2004) Presented a poster in Salk meeting on DNA Replication and Genome Integrity, La Jolla CA (2006) Presented to Department of Pathology, Harvard Medical School (2011) Presented to Data and Journal club of Department of Genetics, Harvard Medical School (2013) PUBLICATIONS Lu T, Aron L, Zullo J, Pan Y, Kim H, Chen Y, Yang TH, Kim HM, Drake D, Liu XS, Bennett DA, Colaiácovo MP, Yankner BA. (2014) REST and stress resistance in aging and Alzheimer's disease. Nature. 507(7493):448-54. Mosammaparast N, Kim H, Laurent B, Zhao Y, Lim HJ, Majid MC, Dango S, Luo Y, Hempel K, Sowa ME, Gygi SP, Steen H, Harper JW, Yankner B, Shi Y. (2013) The histone demethylase LSD1/KDM1A promotes the DNA damage response. J Cell Biol. 203(3):457-70. Kim H, Livingston DM. (2009) Suppression of a DNA polymerase delta mutation by the absence of the high mobility group protein Hmo1 in Saccharomyces cerevisiae. Curr Genet. 55(2):127-38. Kim H, Livingston DM. (2006) A high mobility group protein binds to long CAG repeat tracts and establishes their chromatin organization in Saccharomyces cerevisiae. J Biol Chem. 281(23):15735-40. Myung K, Ghosh G, Fattah FJ, Li G, Kim H, Dutia A, Pak E, Smith S, Hendrickson EA. (2004) Regulation of telomere length and suppression of genomic instability in human somatic cells by Ku86. Mol Cell Biol. 24(11):5050-9. Kim ES, Kim H, Park RD, Lee Y, Han O. (2002) Dual positional specificity of wound-responsive lipoxygenase from maize seedlings. J Plant Physiol. 159(11): 1263-5. Kim C, Kim H, Han O. (2001) The role of serine-246 in cytochrome P450eryF-catalyzed hydroxylation of 6-deoxyerythronolide B. Biosci Biotechnol Biochem. 65(4):752-7.
Skills
editing, ELISA, GC, HPLC, image, Imaging, materials, exchange, neural, pathway, PRISM, Reporter, RT-PCR, safety, Statistical analysis
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Resume Overview

Companies Worked For:

  • Harvard Medical School
  • University of Minnesota
  • Chonnam National University
  • Pohang University of Science and Technology

School Attended

  • University of Minnesota
  • Chonnam National University

Job Titles Held:

  • Research Fellow
  • Research Assistant

Degrees

  • Ph.D : Biochemistry and Molecular Biology , 2007
    M.A : Biochemistry , 2002
    B.A : Genetic Engineering , 1999

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