Research Associate III Resume Example
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RESEARCH ASSOCIATE III
Professional Overview
Committed and detail-oriented biomedical professional experience in multi-parameter flow cytometry, western blotting, cell culture and leukocyte isolation. Responsible for generating lab reports with data analysis, result summary and graph presentation.
Core Qualifications
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Education
Master of Science : Biotechnology, 2005JOHNS HOPKINS UNIVERSITY － City, State
Bachelor of Science : Medical and Research Technology, 1999UNIVERSITY OF MARYLAND, BALTIMORE － City, StateGraduated with High Honor, Excellence in Clinical Chemistry
Experience
Research Associate III
April 2006 to CurrentPlanet Pharma － Santa Ana , CA
April 2006 to CurrentPlanet Pharma － Santa Ana , CA
- Gel electrophoresis and western blot with colormetric and enhanced chemiluminescence methods
- Multi-color flow cytometric evaluation of granulocytes hydrogen peroxide production using dihydrorhodamine (DHR) and the upregulation of various cell surface markers in activated neutrophils
- Culture and cryopreservation of EBV transformed mononuclear cells
- Actin spin-down and polymerization assays to determine the mechanism of cytoskeleton defect in leukocyte
- Immunoprecipitation of granulocyte protein using magnetic beads
- Quantify superoxide generation by activated neutrophils with spectrophotometer and nitroblue tetrazolium (NBT) staining
- Adherence assay to detect neutrophil's ability to adhere to extracellular matrix proteins
- Ficoll cell isolation for PBMC and neutrophil
Research Assistant
May 2001 to April 2006Boyce Thompson Institute － Ithaca , NY
May 2001 to April 2006Boyce Thompson Institute － Ithaca , NY
- Monitored immune functions in patients with HIV infection using multi-parameter flow cytometry
- In vitro BrdU labeling to evaluate the effects of HIV infection on T cell turnover
- Data analysis and quality control procedures
- Staining of leukocytes with fluorochrome-labeled monoclonal antibodies
- Performed clinical hematology testing
- Training of new employees
- Worked in BL-3 laboratory
Medical Technologist
July 1999 to April 2001Catholic Health Initiative － Nicholasville , KY
July 1999 to April 2001Catholic Health Initiative － Nicholasville , KY
- Performed clinical laboratory testing which included chemistry, special chemistry, hematology and urinalysis; responsible for lab management - evaluated Levy-Jennings charts, prepared procedure manual.
Research Technician
August 1998 to June 1999Agricultural & Life Sciences － Madison , WI
August 1998 to June 1999Agricultural & Life Sciences － Madison , WI
- Worked at the cardiac-renal molecular research laboratory; researched on apoptosis on cardiovascular tissue using ELISA; wrote review paper on Apoptosis in Cardiovascular Diseases; poster presentation at Experimental Biology'99.
Awards
- 2012 Outstanding Achievement Award for our group contribution in determining the effectiveness of bone marrow transplants in patients with immunodeficiencies
- 2007 Outstanding Achievement Award for our group contribution in determining the mechanism of IFN-g on superoxide production in patients with chronic granulomatous disease
Publications
- Sen SK, Boelte KC, Barb JJ, Joehanes R, Zhao X, Cheng Q, Adams L, Teer JK, Accame DS, Chowdhury S, Singh LN; NISC Comparative Sequencing Program; CHARGE Consortium, Kavousi M, Peyser PA, Quigley L, Priel DL, Lau K, Kuhns DB, Yoshimura T, Johnson AD, Hwang SJ, Chen MY, Arai AE, Green ED, Mullikin JC, Kolodgie FD, O'Donnell CJ, Virmani R, Munson PJ, McVicar DW, Biesecker LG. Integrative DNA, RNA, and Protein Evidence Connects TREML4 to Coronary Artery Calcification. AJHG 95:66–76, July 2014. Selected as 2014 Cotterman Award.
- Vinh DC, Schwartz B, Hsu AP, Miranda DJ, Valdez PA, Fink D, Lau KP etc. Interleukin-12 Repceptor β1 Deficiency Predisposing to Disseminated Coccidiodomycosis. Clinical Infectious Diseases 2011 Feb 15; 52(4):e99-e102.
- Higgins J., Hill V., Lau K., Simpson V., Roayaei J., Klabansky R., Stevens R.A., Metcalf J. and Baseler B. Evaluation of a Single-Platform Technology for Lymphocyte Immunophenotyping. Clinical and Vaccine Immunology, October 2007, p.1342-1348, Vol. 14, No. 10
Presentations
- Long Priel DA, Fink DL, Lau KP, Mendez LM, Riva DK, Kuhns DB. 2014. p40phox Chronic Granulomatous Disease: A Paradox in Diagnosis. Spring Research Festival, Fort Detrick, NCI FNLCR
- Lau K., Fink, D., D., Kuhns D. 2013. A Novel Assay to Assess IFN-g Enhanced ROS Production in Vitro in Patients with Chronic Granulomatous Disease. Spring Research Festival, Fort Detrick, NCI FCRDC.
- Lau K., Fink, D., Riva, D., Coffin, L., Long Priel D., Kuhns D. 2011. A Heterozygous Mutation in C/EBPε Results in Disrupted Neutrophil Maturation and Specific Granule Deficiency. Spring Research Festival, Fort Detrick, NCI FCRDC.
- Long Priel DA, Riva DK, Coffin LM, Lau KP, Fink DL, Kuhns DB. 2010. A Mutation in PU.1 Binding Region of the CYBB Gene Results in Atypical Chronic Granulomatous Disease with Differential Expression of NAPDH Oxidase in Neutrophils and Eosinophils. Spring Research Festival, Fort Detrick, NCI FCRDC.
- Lau K., Fink, D., Long Priel D., Kuhns D. 2009. Three Color Staining to Differentiate Monocytes and Neutrophils Reactive Oxygen Species Production. Spring Research Festival, Fort Detrick, NCI FCRDC.
- Lau, K., Fink, D., Long Priel D., Gallin J.I. and Kuhns D. 2008. A Pharmacogenomic Study to Predict Effectiveness of IFN-γ Therapy in Chronic Granulomatous Disease. Spring Research Festival, Fort Detrick, NCI FCRDC.
- Lau K., Long Priel
D., and Kuhns D. 2007. Detection
and Quantitation of NADPH Oxidase Subunits in Neutrophils Isolated from Patients
with Chronic Granulomatous Disease using Enhanced Chemiluminescence. Spring
Research Festival, Fort Detrick, NCI FCRDC.
Memberships/Scholarly Societies
Licensed Medical Technologist MT(ASCP)
Member of American Society of Clinical Pathologist
Skills
Laborotory: Familiar with CLIA, HIPPA/HITECH regulations
Computer software: Windows and Microsoft Office, BD Diva (flow cytometry), Prism (Scientific graphing), Softmax (Spectramax Microplate reader)
Language: Chinese
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Resume Overview
School Attended
- JOHNS HOPKINS UNIVERSITY
- UNIVERSITY OF MARYLAND, BALTIMORE
Job Titles Held:
- Research Associate III
- Research Assistant
- Medical Technologist
- Research Technician
Degrees
- Master of Science : Biotechnology , 2005
Bachelor of Science : Medical and Research Technology , 1999
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