Research Associate III Resume Example Leidos Biomedical Inc.

RESEARCH ASSOCIATE III

Professional Overview

Committed and detail-oriented biomedical professional experience in multi-parameter flow cytometry, western blotting, cell culture and leukocyte isolation. Responsible for generating lab reports with data analysis, result summary and graph presentation.

Core Qualifications

Multi-parameter flow cytometry
Western blotting
Cell culture
Leukocyte isolation
ELISA

Microscopic evaluation of leukocyte morphology
Data analysis and result summary
Proficiency in Microsoft Word, Excel and Access
Prepare SOP for laboratory assays

Education

Master of Science : Biotechnology, 2005JOHNS HOPKINS UNIVERSITY － Rockville, MD

Bachelor of Science : Medical and Research Technology, 1999UNIVERSITY OF MARYLAND, BALTIMORE － Baltimore, MDGraduated with High Honor, Excellence in Clinical Chemistry

Experience

Research Associate III
April 2006 to CurrentLeidos Biomedical Inc. － Frederick, MD

Gel electrophoresis and western blot with colormetric and enhanced chemiluminescence methods
Multi-color flow cytometric evaluation of granulocytes hydrogen peroxide production using dihydrorhodamine (DHR) and the upregulation of various cell surface markers in activated neutrophils
Culture and cryopreservation of EBV transformed mononuclear cells
Actin spin-down and polymerization assays to determine the mechanism of cytoskeleton defect in leukocyte
Immunoprecipitation of granulocyte protein using magnetic beads
Quantify superoxide generation by activated neutrophils with spectrophotometer and nitroblue tetrazolium (NBT) staining
Adherence assay to detect neutrophil's ability to adhere to extracellular matrix proteins
Ficoll cell isolation for PBMC and neutrophil

Research Assistant
May 2001 to April 2006SAIC-Frederick － Frederick, MD

Monitored immune functions in patients with HIV infection using multi-parameter flow cytometry
In vitro BrdU labeling to evaluate the effects of HIV infection on T cell turnover
Data analysis and quality control procedures
Staining of leukocytes with fluorochrome-labeled monoclonal antibodies
Performed clinical hematology testing
Training of new employees
Worked in BL-3 laboratory

Medical Technologist
July 1999 to April 2001Richard A. DeSantis MD (Physician office lab) － Bel Air, MD

Performed clinical laboratory testing which included chemistry, special chemistry, hematology and urinalysis; responsible for lab management - evaluated Levy-Jennings charts, prepared procedure manual.

Research Technician
August 1998 to June 1999UNIVERSITY OF MARYLAND BALTIMORE － Baltimore, MD

Worked at the cardiac-renal molecular research laboratory; researched on apoptosis on cardiovascular tissue using ELISA; wrote review paper on Apoptosis in Cardiovascular Diseases; poster presentation at Experimental Biology'99.

Awards

2012 Outstanding Achievement Award for our group contribution in determining the effectiveness of bone marrow transplants in patients with immunodeficiencies
2007 Outstanding Achievement Award for our group contribution in determining the mechanism of IFN-g on superoxide production in patients with chronic granulomatous disease

Publications

Sen SK, Boelte KC, Barb JJ, Joehanes R, Zhao X, Cheng Q, Adams L, Teer JK, Accame DS, Chowdhury S, Singh LN; NISC Comparative Sequencing Program; CHARGE Consortium, Kavousi M, Peyser PA, Quigley L, Priel DL, Lau K, Kuhns DB, Yoshimura T, Johnson AD, Hwang SJ, Chen MY, Arai AE, Green ED, Mullikin JC, Kolodgie FD, O'Donnell CJ, Virmani R, Munson PJ, McVicar DW, Biesecker LG. Integrative DNA, RNA, and Protein Evidence Connects TREML4 to Coronary Artery Calcification. AJHG 95:66–76, July 2014. Selected as 2014 Cotterman Award.
Vinh DC, Schwartz B, Hsu AP, Miranda DJ, Valdez PA, Fink D, Lau KP etc. Interleukin-12 Repceptor β1 Deficiency Predisposing to Disseminated Coccidiodomycosis. Clinical Infectious Diseases 2011 Feb 15; 52(4):e99-e102.
Higgins J., Hill V., Lau K., Simpson V., Roayaei J., Klabansky R., Stevens R.A., Metcalf J. and Baseler B. Evaluation of a Single-Platform Technology for Lymphocyte Immunophenotyping. Clinical and Vaccine Immunology, October 2007, p.1342-1348, Vol. 14, No. 10

Presentations

Long Priel DA, Fink DL, Lau KP, Mendez LM, Riva DK, Kuhns DB. 2014. p40phox Chronic Granulomatous Disease: A Paradox in Diagnosis. Spring Research Festival, Fort Detrick, NCI FNLCR
Lau K., Fink, D., D., Kuhns D. 2013. A Novel Assay to Assess IFN-g Enhanced ROS Production in Vitro in Patients with Chronic Granulomatous Disease. Spring Research Festival, Fort Detrick, NCI FCRDC.
Lau K., Fink, D., Riva, D., Coffin, L., Long Priel D., Kuhns D. 2011. A Heterozygous Mutation in C/EBPε Results in Disrupted Neutrophil Maturation and Specific Granule Deficiency. Spring Research Festival, Fort Detrick, NCI FCRDC.
Long Priel DA, Riva DK, Coffin LM, Lau KP, Fink DL, Kuhns DB. 2010. A Mutation in PU.1 Binding Region of the CYBB Gene Results in Atypical Chronic Granulomatous Disease with Differential Expression of NAPDH Oxidase in Neutrophils and Eosinophils. Spring Research Festival, Fort Detrick, NCI FCRDC.
Lau K., Fink, D., Long Priel D., Kuhns D. 2009. Three Color Staining to Differentiate Monocytes and Neutrophils Reactive Oxygen Species Production. Spring Research Festival, Fort Detrick, NCI FCRDC.
Lau, K., Fink, D., Long Priel D., Gallin J.I. and Kuhns D. 2008. A Pharmacogenomic Study to Predict Effectiveness of IFN-γ Therapy in Chronic Granulomatous Disease. Spring Research Festival, Fort Detrick, NCI FCRDC.
Lau K., Long Priel D., and Kuhns D. 2007. Detection and Quantitation of NADPH Oxidase Subunits in Neutrophils Isolated from Patients with Chronic Granulomatous Disease using Enhanced Chemiluminescence. Spring Research Festival, Fort Detrick, NCI FCRDC.

Memberships/Scholarly Societies

Licensed Medical Technologist MT(ASCP)

Member of American Society of Clinical Pathologist

Skills

Laborotory: Familiar with CLIA, HIPPA/HITECH regulations

Computer software: Windows and Microsoft Office, BD Diva (flow cytometry), Prism (Scientific graphing), Softmax (Spectramax Microplate reader)

Language: Chinese

Resume Overview

Companies Worked For:

Leidos Biomedical Inc.
SAIC-Frederick
Richard A. DeSantis MD (Physician office lab)
UNIVERSITY OF MARYLAND BALTIMORE

School Attended

JOHNS HOPKINS UNIVERSITY
UNIVERSITY OF MARYLAND, BALTIMORE

Job Titles Held:

Research Associate III
Research Assistant
Medical Technologist
Research Technician

Degrees

Master of Science : Biotechnology , 2005
Bachelor of Science : Medical and Research Technology , 1999

Research Associate III Resume Example

Resume Score: 100%

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