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Research Associate II Resume Example

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RESEARCH ASSOCIATE II
https://www.linkedin.com/in/hanieh-hossein-nejad-ariani-21477967/
Professional Summary

Dedicated researcher familiar with peptide/Protein protocols. Well-coordinated in handling study documentation and records. Ready to bring advanced knowledge and skills to enhance research accuracy and precision at pharmaceutical field facility. Skilled researcher specializing in analytical biochemistry and well-versed in biochemistry, biophysics and data analysis. Insightful professional ready to apply 9 years of experience to new role with room for growth and advancement.

Skills
  • 1. Solid phase synthesis of peptides manually Chemical peptide synthesis (Fmoc –Boc strategy) by automated synthesizer protein technology Prelude, Tribute and Respep SL intavis. Synthesis of peptide libraries and peptide array.
  • 2. Peptide conjugates.
  • 3. Reversed Phase-High Performance Liquid Chromatography, C18 & C8 analytical & semiprep columns, ÄKTA Purifier, Waters, Knauer and Shimadzu.
  • 4. Laboratory animal handling.
  • 5. Protein production and purification.
  • 6. FPLC, ÄKTA Purifier.
  • 7. Amino acid Analyzer.
  • 8. SDS-PAGE.
  • 9. Minimum inhibitory concentration (micro dilution method).
  • 10. Hemolytic assay.
  • 11. Nanodrop spectrophotometer.
  • 12. UV-visible Spectroscopy.
  • 13. Circular Dichroism Spectroscopy. 14. Fluorescence Spectroscopy.
  • 15. Potentiometer.
  • 16. Q-Tof1 ESI-MS Spectroscopy.
  • 17. MALDI-TOF.
  • 18. Titration studies using pH meter.
  • 19. CD and UV-visible spectroscopy.
  • 20. Scientific visualization, Yasara, MOLMOL.
  • 21. FACS
  • 22. LCMS Shimadzu
  • 23. Thermal shift
  • 24. Crystal screen
  • 25. ATPase assay
  • 26. Microscale Thermophoresis (MST)
  • 27. Surface Plasmon resonance (SPR)
Work History
02/2019 - CurrentResearch Associate II | Cedars-Sinai Medical Center - Los Angeles, CA
  • Protein expression and purification and performing crystal screens.
  • ATP ase assay and Thermal shift assay.
  • Drug discovery and binding assay studies, performing pilot experiments for grant applications.
09/2018 - 12/2018Senior Analytical Chemist | Testing Company - Irvine, CA
  • Research and method development for analysis of macromolecules using HPLC, mass spectrometry and ELSD.
  • Sugar, phospholipids, etc analysis using ELSD.
  • Training junior chemists, writing SOP and report.
03/2016 - 09/2018Postdoctoral Researcher | Chapman University - Irvine, CA
  • In Chapman University School of Pharmacy, CUSP California My position as a postdoctoral researcher (2016-2018) was supported by the Chapman University School of Pharmacy.
  • Since I work in pharmacy school we practice to work in GMP and non-GMP environments.
  • My focus was on chemical synthesis of therapeutic peptides and conjugations which enable us to recognize the cancer cells selectively and efficiently.
  • The peptides I synthesized include cyclic, linear, FITC labeled peptides.
  • In other projects, we optimized diagnostic tests for the detection of prokaryotic and eukaryotic abnormal cells, such as circulating tumor cells by applying potential antibacterial peptides.
  • Also I have extensive experience on analytical instruments like RP-HPLC (analytical, semiprep, preparative), MALDI-MS and peptide synthesizers like Prelude and Tribute form protein Technology and peptide array synthesis.
  • Moreover, I mentored undergraduate and graduate students at lab and have gained valuable management skills.
Education
Tarbiat Modares University, TehranMasters: Title of
Ferdowsi University of MashhadBachelor of Science: Biological Sciences (Animal Biology)Title of B.Sc thesis: The effect of vitreous matrix on wounds of rabbit ear.
02/2014Institute of Biophysics And Biochemistry Ph.D.: Biochemistry And Biophysics

thesis: Structural aspects of Ni (II)-assisted peptide bond hydrolysis.

Accomplishments

Patent:

(WO2019099467) RAPID SELECTIVE DETECTION OF BACTERIA

Publications:

1) Inorganic chemistry(ACS Publications) Effect of D-Amino Acid Substitutions on Ni(II)-Assisted Peptide Bond Hydrolysis .Hanieh H. Ariani, Agnieszka Polkowska-Nowakowska, and Wojciech Bal. 2013.

2) Journal of Inorganic Biochemistry. Sequence-Specific Ni(II)-Dependent Peptide Bond Hydrolysis for Protein Engineering: Active Sequence Optimization. Anna Maria Protas, Hanieh H. Ariani ,Arkadiusz Bonna, Agnieszka Polkowska-Nowakowska, Jarosław Poznański,Wojciech Bal, 2013.

3) Journal of Food Processing and Preservation. Antioxidant and Angiotensin I Converting Enzyme (ACE) Inhibitory Properties of GL-9 Peptide. Hoda Shabestarian, Ahmad Asoodeh, Masoud Homayouni-Tabrizi and Hanieh H.Ariani, 2016.

4) Applied nanomaterial ACS journal Peptide-based biosensor utilizing fluorescent gold nanoclusters for detection of Listeria monocytogenes. Hanieh Hossein-Nejad-Ariani, Tushar Kim, and Kamaljit Kaur, 2018.

5) Scientific Reports Small Peptide Ligands for Targeting EGFR in Triple Negative Breast Cancer Cells. Hanieh Hossein-Nejad-Ariani, Emad Althaghafi, and Kamaljit Kaur, 2019.

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Resume Overview

Companies Worked For:

  • Cedars-Sinai Medical Center
  • Testing Company
  • Chapman University

School Attended

  • Tarbiat Modares University
  • Ferdowsi University of Mashhad
  • Institute of Biophysics And Biochemistry

Job Titles Held:

  • Research Associate II
  • Senior Analytical Chemist
  • Postdoctoral Researcher

Degrees

  • Masters :
    Bachelor of Science : Biological Sciences (Animal Biology)
    Ph.D. : Biochemistry And Biophysics

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